O84649 (O84649_CHLTR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA Topoisomerase I-Fused to SWI Domain EMBL AAC68820.1

Gene names

Name:	topA EMBL AAC68820.1
Ordered Locus Names:	CT_643

Organism

Chlamydia trachomatis

Taxonomic identifier

813 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	857 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	O84649 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 5ABD780B5DBBB562	FASTA	857	96,704
	10 20 30 40 50 60 MKKSLIIVES PAKIKTLRKL LGEGFIFDSS LGHIVDLPAK GFGIDIENGF VPDYQILEGK 70 80 90 100 110 120 KEVIRKICAE AKKCDVVYLA PDPDREGEAI AWHIANQLPK DTKIQRISFN AITKGAVTEA 130 140 150 160 170 180 LKHPREIDMA LVNAQQARRF LDRIVGYKIS PILGRKLQRW SGVSAGRVQS VALKLVVDRE 190 200 210 220 230 240 YAIERFVPVE FWNIRVHLKD PQTQKTFWAH LHSVNGKKWE KEIPEGKTSD EVILIDSKEK 250 260 270 280 290 300 ADEIVALLES ATYVVDRVES KEKKRHAYPP FITSTLQQEA SRHYRFSSSR TMNIAQTLYE 310 320 330 340 350 360 GVDLDSQGAV GLITYMRTDS VRTDPEAVKQ VRKYIEGHFG KEFVPSSPNV YATKKMAQDA 370 380 390 400 410 420 HEAIRPTDVT ITPESIRSKL TEDQYKLYSL IWKRFVASQM ISAIYDTLAI RITTNKGIDL 430 440 450 460 470 480 RATGSCLKFK GFLAVYEEKR DEEGDEEENI HLPKLNERDV LTKEELEAEQ SHTKPLPRFT 490 500 510 520 530 540 EASLVKELEK SGIGRPSTYA TIMNKIQSRE YTLKEGQRLR PTELGKVVCQ FLETNFPRIM 550 560 570 580 590 600 DIGFTAGMED ELELIADNKK PWKQLLQEFC ELFLPFVVTA EKEAFIPRIV TEIDCPKCHK 610 620 630 640 650 660 GKLVKIWAKN RYFFGCSEYP TCDYKTSEEE LTFDKNEYAE DTPWDAPCAL CGGEMKVRHG 670 680 690 700 710 720 KFGSFLGCEN YPKCHYIVNL FKKGEAGAEP EATVHCPAEG CTGHLVKRRS RFNKMFYSCS 730 740 750 760 770 780 EYPACSVIGN SVDAVIEKYA GTPKTPYEKK PKAKKSIAST KGKAAKTVKK SSATTKKRAT 790 800 810 820 830 840 KAYTPSAALA AVIGADPVGR PEATKKLWEY IKEKGLQSPQ NKKIIIPDSK LQGVIGADPI 850 DMFALSKKLS AHLIKEE

References

[1]	"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis." Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W. Science 282:754-759(1998) [PubMed: 9784136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D/UW-3/Cx.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001273 Genomic DNA. Translation: AAC68820.1.
PIR	A71488.
RefSeq	NP_220161.1. NC_000117.1.
3D structure databases
HSSP	HSSP built from PDB template 1ECL based on UniProtKB P06612.
ProteinModelPortal	O84649.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	884426.
GenomeReviews	Gene locus CT_643 in contig AE001273_GR.
KEGG	ctr:CT643.
PATRIC	20380928. VBIChlTra43535_0707.
Phylogenomic databases
HOGENOM	HBG512935.
OMA	EEYWSID.
PhylomeDB	O84649.
ProtClustDB	PRK06319.
Family and domain databases
InterPro	IPR019835. SWIB_domain. IPR003121. SWIB_MDM2_domain. IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF02201. SWIB. 1 hit. PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00151. SWIB. 1 hit. SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF47592. MDM2. 1 hit. SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

O84649_CHLTR

Accession

Primary (citable) accession number: O84649

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1998
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)