O84638 (HEM2_CHLTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Delta-aminolevulinic acid dehydratase
Short name=ALAD
Short name=ALADH
EC=4.2.1.24

Alternative name(s):
Porphobilinogen synthase

Gene names

Name:	hemB
Ordered Locus Names:	CT_633

Organism

Chlamydia trachomatis

Taxonomic identifier

813 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.
Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Delta-aminolevulinic acid dehydratase		PRO_0000140498
Sites
	Active site	199	1	Schiff-base intermediate with substrate By similarity
	Active site	252	1	Schiff-base intermediate with substrate By similarity
	Metal binding	237	1	Magnesium By similarity
	Binding site	209	1	Substrate 1 By similarity
	Binding site	221	1	Substrate 1 By similarity
	Binding site	278	1	Substrate 2 By similarity
	Binding site	317	1	Substrate 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	O84638 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 83E928F68EAA63FB	FASTA	338	37,737
	10 20 30 40 50 60 MIRLPLLKRP RRNRKSAAVR SIIQETQLCS SDLIWPIFLK DGSGIREEIK SMPGVYRWSL 70 80 90 100 110 120 DMVSKELERL CTIGLKAVIL FPVIDANKKE QFGSYASHPY NIVCKGIQAI KKSFPELCVI 130 140 150 160 170 180 SDIALDPFTT SGHDGIFHNN YVINDESVRV YGGIAVMHAE MGADIVAPSD MMDGRVKHIR 190 200 210 220 230 240 EQMDQMGFVN TGILSYSAKY ASALYGPFRD ALSSHLQSGD KRTYQMDPAN VQEALLECQL 250 260 270 280 290 300 DEEEGADMVM IKPAGFYLDV IVKARENTHL PVVAYQVSGE FSMIMAACLH GWLNKESVIK 310 320 330 ESLLAIKRAG ATAIISYATP WVLEWLAKDA LPFERSVL

References

[1]	"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis." Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W. Science 282:754-759(1998) [PubMed: 9784136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D/UW-3/Cx.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001273 Genomic DNA. Translation: AAC68237.1.
PIR	E71489.
RefSeq	NP_220150.1. NC_000117.1.
3D structure databases
ProteinModelPortal	O84638.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	884414.
GenomeReviews	Gene locus CT_633 in contig AE001273_GR.
KEGG	ctr:CT633.
PATRIC	20380898. VBIChlTra43535_0693.
Phylogenomic databases
HOGENOM	HBG285270.
OMA	FTSHGHD.
ProtClustDB	PRK09283.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001731. Porphobilinogen_synth. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01698.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
SMART	SM01004. ALAD. 1 hit. [Graphical view]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM2_CHLTR

Accession

Primary (citable) accession number: O84638

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program