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Protein

Aspartate--tRNA(Asp/Asn) ligase

Gene

aspS

Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).UniRule annotation1 Publication

Catalytic activityi

ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation1 Publication

Kineticsi

  1. KM=0.95 µM for tRNA(Asp)1 Publication
  2. KM=2.76 µM for tRNA(Asn)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei32 – 321Important for tRNA non-discriminationUniRule annotation
    Sitei84 – 841Important for tRNA non-discriminationUniRule annotation
    Binding sitei177 – 1771AspartateUniRule annotation
    Binding sitei223 – 2231AspartateUniRule annotation
    Binding sitei232 – 2321ATPUniRule annotation
    Binding sitei447 – 4471AspartateUniRule annotation
    Binding sitei481 – 4811ATPUniRule annotation
    Binding sitei484 – 4841AspartateUniRule annotation
    Binding sitei488 – 4881AspartateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi223 – 2253ATPUniRule annotation
    Nucleotide bindingi533 – 5364ATPUniRule annotation

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)
    Alternative name(s):
    Aspartyl-tRNA synthetaseUniRule annotation
    Short name:
    AspRSUniRule annotation
    Non-discriminating aspartyl-tRNA synthetaseUniRule annotation
    Short name:
    ND-AspRSUniRule annotation
    Gene namesi
    Name:aspSUniRule annotation
    Ordered Locus Names:CT_542
    OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
    Taxonomic identifieri272561 [NCBI]
    Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
    Proteomesi
    • UP000000431 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 582582Aspartate--tRNA(Asp/Asn) ligasePRO_0000110856Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272561.CT542.

    Structurei

    3D structure databases

    ProteinModelPortaliO84546.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni201 – 2044AspartateUniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105C9M. Bacteria.
    COG0173. LUCA.
    InParanoidiO84546.
    KOiK01876.
    OMAiYQLDVEM.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.1360.30. 1 hit.
    HAMAPiMF_00044. Asp_tRNA_synth. 1 hit.
    InterProiIPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004524. Asp-tRNA-ligase_bac/mit.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR004115. GAD-like.
    IPR029351. GAD_dom.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view]
    PANTHERiPTHR22594. PTHR22594. 2 hits.
    PTHR22594:SF5. PTHR22594:SF5. 2 hits.
    PfamiPF02938. GAD. 1 hit.
    PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view]
    PRINTSiPR01042. TRNASYNTHASP.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF55261. SSF55261. 1 hit.
    TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O84546-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKYRTHKCNE LSLDHVGEHV RLSGWVHRYR NHGGVVFIDL RDCFGITQIV
    60 70 80 90 100
    CRQEENPELH QLMDQVRSEW VLCVEGLVCA RLEGMENPNL VTGSIEVEVS
    110 120 130 140 150
    SLEVLSRAQN LPFSISDEHI NVNEELRLTY RYLDMRRGDI LDRLMCRHKV
    160 170 180 190 200
    MLACRQYLDE QGFTEVVTPI LGKSTPEGAR DYLVPSRIYP GNFYALPQSP
    210 220 230 240 250
    QLFKQILMVG GLDRYFQIAT CFRDEDLRAD RQPEFTQIDM EMSFGGPEDL
    260 270 280 290 300
    FPVVEELVAR LFAVKGIELK APFLRMTYQE AKDSYGTDKP DLRFGLRLKN
    310 320 330 340 350
    CCEYARKFTF SIFLDQLAYG GTVKGFCVPG GADMSRKQLD IYTDFVKRYG
    360 370 380 390 400
    AMGLVWIKKQ DGGVSSNVAK FASEDVFQEM FEAFEAKDQD ILLLIAAPEA
    410 420 430 440 450
    VANQALDHLR RLIARERQLY DSTQYNFVWI TDFPLFAKEE GELCPEHHPF
    460 470 480 490 500
    TAPLDEDISL LDSDPFAVRS SSYDLVLNGY EIASGSQRIH NPDLQNKIFA
    510 520 530 540 550
    LLKLSQESVK EKFGFFIDAL SFGTPPHLGI ALGLDRIMMV LTGAETIREV
    560 570 580
    IAFPKTQKAG DLMMSAPSEI LPIQLKELGL KL
    Length:582
    Mass (Da):66,227
    Last modified:November 1, 1998 - v1
    Checksum:iE8D2EBB2785EFABA
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE001273 Genomic DNA. Translation: AAC68144.1.
    PIRiG71500.
    RefSeqiNP_220057.1. NC_000117.1.
    WP_009871906.1. NC_000117.1.

    Genome annotation databases

    EnsemblBacteriaiAAC68144; AAC68144; CT_542.
    GeneIDi884319.
    KEGGictr:CT_542.
    PATRICi20380681. VBIChlTra43535_0587.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE001273 Genomic DNA. Translation: AAC68144.1.
    PIRiG71500.
    RefSeqiNP_220057.1. NC_000117.1.
    WP_009871906.1. NC_000117.1.

    3D structure databases

    ProteinModelPortaliO84546.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi272561.CT542.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC68144; AAC68144; CT_542.
    GeneIDi884319.
    KEGGictr:CT_542.
    PATRICi20380681. VBIChlTra43535_0587.

    Phylogenomic databases

    eggNOGiENOG4105C9M. Bacteria.
    COG0173. LUCA.
    InParanoidiO84546.
    KOiK01876.
    OMAiYQLDVEM.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.1360.30. 1 hit.
    HAMAPiMF_00044. Asp_tRNA_synth. 1 hit.
    InterProiIPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004524. Asp-tRNA-ligase_bac/mit.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR004115. GAD-like.
    IPR029351. GAD_dom.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view]
    PANTHERiPTHR22594. PTHR22594. 2 hits.
    PTHR22594:SF5. PTHR22594:SF5. 2 hits.
    PfamiPF02938. GAD. 1 hit.
    PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view]
    PRINTSiPR01042. TRNASYNTHASP.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF55261. SSF55261. 1 hit.
    TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiSYDND_CHLTR
    AccessioniPrimary (citable) accession number: O84546
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1998
    Last modified: September 7, 2016
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.