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Protein

Aspartate--tRNA(Asp/Asn) ligase

Gene

aspS

Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).UniRule annotation1 Publication

Catalytic activityi

ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation1 Publication

Kineticsi

  1. KM=0.95 µM for tRNA(Asp)1 Publication
  2. KM=2.76 µM for tRNA(Asn)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei32Important for tRNA non-discriminationUniRule annotation1
    Sitei84Important for tRNA non-discriminationUniRule annotation1
    Binding sitei177AspartateUniRule annotation1
    Binding sitei223AspartateUniRule annotation1
    Binding sitei232ATPUniRule annotation1
    Binding sitei447AspartateUniRule annotation1
    Binding sitei481ATPUniRule annotation1
    Binding sitei488AspartateUniRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi223 – 225ATPUniRule annotation3
    Nucleotide bindingi533 – 536ATPUniRule annotation4

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAminoacyl-tRNA synthetase, Ligase
    Biological processProtein biosynthesis
    LigandATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)
    Alternative name(s):
    Aspartyl-tRNA synthetaseUniRule annotation
    Short name:
    AspRSUniRule annotation
    Non-discriminating aspartyl-tRNA synthetaseUniRule annotation
    Short name:
    ND-AspRSUniRule annotation
    Gene namesi
    Name:aspSUniRule annotation
    Ordered Locus Names:CT_542
    OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
    Taxonomic identifieri272561 [NCBI]
    Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
    Proteomesi
    • UP000000431 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001108561 – 582Aspartate--tRNA(Asp/Asn) ligaseAdd BLAST582

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliO84546.
    SMRiO84546.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni201 – 204AspartateUniRule annotation4

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105C9M. Bacteria.
    COG0173. LUCA.
    InParanoidiO84546.
    KOiK01876.
    OMAiYQLDVEM.

    Family and domain databases

    HAMAPiMF_00044. Asp_tRNA_synth_type1. 1 hit.
    InterProiView protein in InterPro
    IPR004364. aa-tRNA-synt_II.
    IPR006195. aa-tRNA-synth_II.
    IPR004524. Asp-tRNA-ligase_1.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR004115. GAD-like_sf.
    IPR029351. GAD_dom.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    PANTHERiPTHR22594:SF5. PTHR22594:SF5. 1 hit.
    PfamiView protein in Pfam
    PF02938. GAD. 1 hit.
    PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    PRINTSiPR01042. TRNASYNTHASP.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF55261. SSF55261. 1 hit.
    TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
    PROSITEiView protein in PROSITE
    PS50862. AA_TRNA_LIGASE_II. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O84546-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKYRTHKCNE LSLDHVGEHV RLSGWVHRYR NHGGVVFIDL RDCFGITQIV
    60 70 80 90 100
    CRQEENPELH QLMDQVRSEW VLCVEGLVCA RLEGMENPNL VTGSIEVEVS
    110 120 130 140 150
    SLEVLSRAQN LPFSISDEHI NVNEELRLTY RYLDMRRGDI LDRLMCRHKV
    160 170 180 190 200
    MLACRQYLDE QGFTEVVTPI LGKSTPEGAR DYLVPSRIYP GNFYALPQSP
    210 220 230 240 250
    QLFKQILMVG GLDRYFQIAT CFRDEDLRAD RQPEFTQIDM EMSFGGPEDL
    260 270 280 290 300
    FPVVEELVAR LFAVKGIELK APFLRMTYQE AKDSYGTDKP DLRFGLRLKN
    310 320 330 340 350
    CCEYARKFTF SIFLDQLAYG GTVKGFCVPG GADMSRKQLD IYTDFVKRYG
    360 370 380 390 400
    AMGLVWIKKQ DGGVSSNVAK FASEDVFQEM FEAFEAKDQD ILLLIAAPEA
    410 420 430 440 450
    VANQALDHLR RLIARERQLY DSTQYNFVWI TDFPLFAKEE GELCPEHHPF
    460 470 480 490 500
    TAPLDEDISL LDSDPFAVRS SSYDLVLNGY EIASGSQRIH NPDLQNKIFA
    510 520 530 540 550
    LLKLSQESVK EKFGFFIDAL SFGTPPHLGI ALGLDRIMMV LTGAETIREV
    560 570 580
    IAFPKTQKAG DLMMSAPSEI LPIQLKELGL KL
    Length:582
    Mass (Da):66,227
    Last modified:November 1, 1998 - v1
    Checksum:iE8D2EBB2785EFABA
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE001273 Genomic DNA. Translation: AAC68144.1.
    PIRiG71500.
    RefSeqiNP_220057.1. NC_000117.1.
    WP_009871906.1. NC_000117.1.

    Genome annotation databases

    EnsemblBacteriaiAAC68144; AAC68144; CT_542.
    GeneIDi884319.
    KEGGictr:CT_542.
    PATRICifig|272561.5.peg.587.

    Similar proteinsi

    Entry informationi

    Entry nameiSYDND_CHLTR
    AccessioniPrimary (citable) accession number: O84546
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1998
    Last modified: November 22, 2017
    This is version 119 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families