Reviewed, UniProtKB/Swiss-Prot O84375 (ARODE_CHLTR)

Names and origin

Protein names

Recommended name:
    Shikimate biosynthesis protein aroDE
Including the following 2 domains:
    1- Recommended name:
            3-dehydroquinate dehydratase
                Short name=3-dehydroquinase
              EC=4.2.1.10
        Alternative name(s):
            Type I DHQase
    2- Recommended name:
            Shikimate dehydrogenase
              EC=1.1.1.25

Gene names

Name:	aroE
Synonyms:	aroD/E
Ordered Locus Names:	CT_370

Organism

Chlamydia trachomatis [Complete proteome] [HAMAP]

Taxonomic identifier

813 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes two sequential steps of the aromatic amino acids biosynthetic pathway. The first reaction is catalyzed by the 3-dehydroquinate dehydratase, coded by the aroD domain; the second reaction is catalyzed by the shikimate 5-dehydrogenase, coded by the aroE domain. HAMAP MF_00214
Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214 Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 3/7. HAMAP MF_00214 Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 4/7.
Sequence similarities	In the N-terminal section; belongs to the type-I 3-dehydroquinase family. In the C-terminal section; belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP Schiff base
Molecular function	Lyase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: HAMAP NADP or NADPH binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Shikimate biosynthesis protein aroDE HAMAP MF_00214		PRO_0000138824
Regions
	Nucleotide binding	337 – 341	5	NADP By similarity
	Region	1 – 208	208	3-dehydroquinate dehydratase HAMAP MF_00214
	Region	209 – 478	270	Shikimate 5-dehydrogenase HAMAP MF_00214
	Compositional bias	137 – 140	4	Poly-Ser HAMAP MF_00214
Sites
	Active site	110	1	Proton acceptor By similarity
	Active site	133	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O84375-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 200DB65E1B081D53	FASTA	478	53,098
	10 20 30 40 50 60 MLCTTISGPS FLEAKKQILR SLKECHCFEM RVDLLSVSCL ELKKLMELAP ISILAWKKPE 70 80 90 100 110 120 SCSQADWIDK MQSLAELNPN YLDLEKDFPE EDMIRIRQLH PQIKIIRSLH TSEHTDIIQL 130 140 150 160 170 180 YAHMRSSAAD YYKFAVSSSS TTDLLDICHQ KRSLPENTTV VCLGGMGRPS RILSPILQNP 190 200 210 220 230 240 FTYARSTGSS PVAPGQFSLK HHYFYNFASL SAQSPICALI GDTSRSIGHL THNPFFSQLG 250 260 270 280 290 300 VACPYIKLPL TPQELPKFFS TIRTQPFLGV SVTSPLKTAV LPFLDKQAPS VKASGSCNTL 310 320 330 340 350 360 VIRQGEIEGH DTDGEGLFSV LMQHQIPLNN QRVAIIGAGG AAQSIATRLS RANCELLIFN 370 380 390 400 410 420 RTKAHAEDLA SRCQAKAFSL EELPLHRVSL IINCLPPSCT IPKAVAPCVV DINTIPKHST 430 440 450 460 470 FTQYARSQGS SIIYGHEMFT QQALLQFRLW FPTLSFKHLE KTFIRRAAVL ASLFSIAP

References

[1]

"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis." Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W. Science 282:754-759(1998) [PubMed: 9784136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D/UW-3/Cx.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001273 Genomic DNA. Translation: AAC67966.1.
PIR	C71523.
RefSeq	NP_219879.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	884748.
GenomeReviews	Gene locus CT_370 in contig AE001273_GR.
KEGG	ctr:CT370.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O84375.
OMA	O84375. ISHLSHN.
Enzyme and pathway databases
BioCyc	CTRA315277:CT370-MON.
BRENDA	1.1.1.25. 108. 4.2.1.10. 108.
Family and domain databases
HAMAP	MF_00214. Fused. [Tree] MF_00222. Fused. [Tree]
InterPro	IPR018508. 3-dehydroquinate_DH_AS. IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. IPR016040. NAD(P)-bd_dom. IPR011342. Quinate/shikimate_5-DH. IPR013708. Shikimate_DH-bd_N. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01487. DHquinase_I. 1 hit. PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
ProDom	PD005337. DHquinase_I. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00507. aroE. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARODE_CHLTR

Accession

Primary (citable) accession number: O84375

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)