Reviewed, UniProtKB/Swiss-Prot O84340 (PT1_CHLTR)

Names and origin

Protein names

Recommended name:
    Phosphoenolpyruvate-protein phosphotransferase
    EC=2.7.3.9
Alternative name(s):
    Phosphotransferase system, enzyme I

Gene names

Name:	ptsI
Ordered Locus Names:	CT_336

Organism

Chlamydia trachomatis [Complete proteome] [HAMAP]

Taxonomic identifier

813 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr) By similarity.
Catalytic activity	Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine.
Cofactor	Magnesium By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	The N-terminal domain contains the HPr binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site By similarity.
Miscellaneous	The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain By similarity.
Sequence similarities	Belongs to the PEP-utilizing enzyme family.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate-protein phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Phosphoenolpyruvate-protein phosphotransferase		PRO_0000147066
Sites
	Active site	207	1	Tele-phosphohistidine intermediate By similarity
	Active site	506	1	Proton donor By similarity
	Metal binding	435	1	Magnesium By similarity
	Metal binding	459	1	Magnesium By similarity
	Binding site	312	1	Substrate By similarity
	Binding site	348	1	Substrate By similarity
	Binding site	435	1	Substrate By similarity
	Binding site	456	1	Substrate; via carbonyl oxygen By similarity
	Binding site	457	1	Substrate; via amide nitrogen By similarity
	Binding site	458	1	Substrate By similarity
	Binding site	459	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	O84340-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 73EBFEEE7C457067	FASTA	571	63,787
	10 20 30 40 50 60 MSVTGQDNKE LQQEFVIVGE PIVPGIGLGK ALLLGKSSLR IRELTLPQEE VEHEISRYYK 70 80 90 100 110 120 ALKRSRSDLA ALEKEAKGKQ GYQEIASILQ AHLEIIKDPL LTEEVVKTIR KDRKNAEFVF 130 140 150 160 170 180 SSVMGEIEKS LCAVQKTTAT RVDRVQDIHD ISNRVIGHLC CQHKSSLGEF DQNLIVFSEE 190 200 210 220 230 240 LTPSEAANAN PEYIRGFVSL EGAKTSHTAI VSLAKNIPYV ANFTTELWDT IKEFSGTLVL 250 260 270 280 290 300 INGDKGEITF NPQLSTIQTY YRKQASVSVT VPVQVQTGKN LPLISLSAQI VSTEELPMIE 310 320 330 340 350 360 RESPGTSVGL FRSEFMAFSL GRLPCVEEQA DQYAQLVQFQ CSDIHVLRLF DFGEDKECPC 370 380 390 400 410 420 ISSSHRSVRW LLEQEKVLKE QLQAIAIVSR IGRLKVLIPG VIDASEIALV KRLFQEEIRL 430 440 450 460 470 480 LKGISENILW GSMIEIPSAV WMIEEILQES SFVALGTNDL AQYTLGTSRE RSLLGERSRV 490 500 510 520 530 540 PHPSVIRMIH HVVEQAKQKN VPVSVCGEMA GDPALLPMFL GLGVKELSAV IPAINSLKMR 550 560 570 LLDLNSRECS RLTKQLLRAK TYEEVHQLLY V

References

[1]

"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis." Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W. Science 282:754-759(1998) [PubMed: 9784136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D/UW-3/Cx.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001273 Genomic DNA. Translation: AAC67931.1.
PIR	C71528.
RefSeq	NP_219843.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	884783.
GenomeReviews	Gene locus CT_336 in contig AE001273_GR.
KEGG	ctr:CT336.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O84340.
OMA	IFRASHY.
Enzyme and pathway databases
BioCyc	CTRA315277:CT336-MON.
BRENDA	2.7.3.9. 108.
Family and domain databases
InterPro	IPR008279. PEP_mobile. IPR018274. PEP_mobile_CS. IPR006318. PEP_P_trans. IPR000121. PEP_utilizers. IPR008731. PTS_PEP_utilis_N. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.50.30.10. PEP_mobile. 1 hit. G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
Pfam	PF05524. PEP-utilisers_N. 1 hit. PF00391. PEP-utilizers. 1 hit. PF02896. PEP-utilizers_C. 1 hit. [Graphical view]
PRINTS	PR01736. PHPHTRNFRASE.
ProDom	PD000940. PEP_utilizers. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01417. PTS_I_fam. 1 hit.
PROSITE	PS00742. PEP_ENZYMES_2. 1 hit. PS00370. PEP_ENZYMES_PHOS_SITE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PT1_CHLTR

Accession

Primary (citable) accession number: O84340

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)