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Protein

Valine--tRNA ligase

Gene

valS

Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.UniRule annotation

Catalytic activityi

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei566 – 5661ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Valine--tRNA ligaseUniRule annotation (EC:6.1.1.9UniRule annotation)
Alternative name(s):
Valyl-tRNA synthetaseUniRule annotation
Short name:
ValRSUniRule annotation
Gene namesi
Name:valSUniRule annotation
Ordered Locus Names:CT_302
OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
Taxonomic identifieri272561 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000431 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 939939Valine--tRNA ligasePRO_0000106222Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272561.CT302.

Structurei

3D structure databases

ProteinModelPortaliO84304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili874 – 93966UniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionAdd
BLAST
Motifi563 – 5675"KMSKS" region

Domaini

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.UniRule annotation
The C-terminal coiled-coil domain is crucial for aminoacylation activity.UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105CA4. Bacteria.
COG0525. LUCA.
InParanoidiO84304.
KOiK01873.
OMAiHNVVEPY.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERiPTHR11946:SF5. PTHR11946:SF5. 4 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF46589. SSF46589. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O84304-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEDQFPKAY DPKSSETGVY SFWERSGMFV ANASSEKPAY SIVMPPPNVT
60 70 80 90 100
GILHMGHALV NTLQDTLIRY KRMQGFEVCW VPGTDHAGIA TQTVVERHLK
110 120 130 140 150
ASLGKQRTDF SREEFLKHVW DWKEKSQNVI LSQLRQLGCS CDWSRQRFTM
160 170 180 190 200
DPGANRAVKK AFKILFDKGV IYRGYYLVNW DPILQTALAD DEVEYEERDG
210 220 230 240 250
WLYYIRYQVV NSEEFITVAT TRPETLLGDT AIAVSPEDLR YSHLIGAKVV
260 270 280 290 300
VPFVNREIPI IGDFSVDASF GTGAVKITPA HDKDDYKTGM NHQLPMINIL
310 320 330 340 350
TPTGEINENG GIFTGLSREV ARENIITSLE ALGLFVKKEA YSSRVGVSYR
360 370 380 390 400
SGAIIEPYLS KQWFVSVDSF RDSLRGFVNS EEIRIFPPEF VRNYLTWVNN
410 420 430 440 450
LKDWCISRQL WWGHRIPVWH NKHDENVICF DGEGGPEEVM RDPESWYQDP
460 470 480 490 500
DVLDTWFSSG LWPLTCFGWP DENSLDLKKF YPTAVLVTGH DILFFWVTRM
510 520 530 540 550
VLMCSAMVDT EPFSDVFLHG LIFGKSYREY DEKGEWFYVS GERKRDYDKG
560 570 580 590 600
KALPKNVVAK WEKLSKSKGN VIDPIEMIEA YGADAVRLTL CSCANRGEQI
610 620 630 640 650
DLDYHLFEEY KNFINKLWNG ARFIFGHISE LTSRDLEEGV NQDLLGLEDF
660 670 680 690 700
YILDRFNELL DLIDGHYNCY SFDKIASLAY DFFKNDLCST YLEIIKPTLF
710 720 730 740 750
GKQDSDQQRA TKRKLLATLL INILGVLHPI VPYITETLFQ KLKATLGTVE
760 770 780 790 800
NGKGDSVTGH AVSMLRSEAC MVAEYPKPIH VAFPQGLRES FGIAERLVYT
810 820 830 840 850
IRNIRGEMQL DPREPLQAFV ISSEKKELVD VCIPIMCALG GVKTVEQLAE
860 870 880 890 900
APKDSIFSLG VVEGIQVGVI LPPEHLAKER VRLEKEKTRL EKSIDSVSKL
910 920 930
LASEDFRTRA NPSLVQAKKD SLRNSQRELQ SILDKLASL
Length:939
Mass (Da):107,036
Last modified:November 1, 1998 - v1
Checksum:iCEB8449DC7BB9066
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC67895.1.
PIRiH71532.
RefSeqiNP_219807.1. NC_000117.1.
WP_009872283.1. NC_000117.1.

Genome annotation databases

EnsemblBacteriaiAAC67895; AAC67895; CT_302.
GeneIDi884822.
KEGGictr:CT_302.
PATRICi20380129. VBIChlTra43535_0323.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC67895.1.
PIRiH71532.
RefSeqiNP_219807.1. NC_000117.1.
WP_009872283.1. NC_000117.1.

3D structure databases

ProteinModelPortaliO84304.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272561.CT302.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC67895; AAC67895; CT_302.
GeneIDi884822.
KEGGictr:CT_302.
PATRICi20380129. VBIChlTra43535_0323.

Phylogenomic databases

eggNOGiENOG4105CA4. Bacteria.
COG0525. LUCA.
InParanoidiO84304.
KOiK01873.
OMAiHNVVEPY.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERiPTHR11946:SF5. PTHR11946:SF5. 4 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF46589. SSF46589. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYV_CHLTR
AccessioniPrimary (citable) accession number: O84304
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.