O84301 (HEM3_CHLTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable porphobilinogen deaminase
Short name=PBG
EC=2.5.1.61

Alternative name(s):
Hydroxymethylbilane synthase
Short name=HMBS
Pre-uroporphyrinogen synthase

Gene names

Name:	hemC
Ordered Locus Names:	CT_299

Organism

Chlamydia trachomatis

Taxonomic identifier

813 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps By similarity. HAMAP MF_00260
Catalytic activity	4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3. HAMAP MF_00260
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4. HAMAP MF_00260
Sequence similarities	Belongs to the HMBS family.
Caution	This sequence may not function as a hydroxymethylbilane synthase because it lacks the cysteine residue necessary for attachment of the dipyrromethane cofactor.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hydroxymethylbilane synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Probable porphobilinogen deaminase HAMAP MF_00260		PRO_0000143019

Sequences

	Sequence		Length	Mass (Da)
	O84301 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: F89FE00C20BF7044	FASTA	241	26,996
	10 20 30 40 50 60 MLSAYYNDPF LADFCLGNIP LRLASRQSSL AVLQAHECLR KLQIFFPRLW GQIITTTTQG 70 80 90 100 110 120 DLDQETPLCA VENTGFFTDD VDFLVQSGQC DLGIHSAKDL PENPKATVVS ITASIDPRDI 130 140 150 160 170 180 LVFHEKYLSI PLPRRLRIGS SSVRRKELLS LLYPSAIITD IRGTIQTRLK LLEEKNFDAI 190 200 210 220 230 240 VMANAAVSRL GLRLPCTKIL PPPYHPLQGR LAITASRHIR SWRGLFLTCG ITEDVEIMCF S

References

[1]	"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis." Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W. Science 282:754-759(1998) [PubMed: 9784136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D/UW-3/Cx.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001273 Genomic DNA. Translation: AAC67892.1.
PIR	E71532.
RefSeq	NP_219804.1. NC_000117.1.
3D structure databases
ProteinModelPortal	O84301.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	884823.
GenomeReviews	Gene locus CT_299 in contig AE001273_GR.
KEGG	ctr:CT299.
PATRIC	20380125. VBIChlTra43535_0321.
Phylogenomic databases
HOGENOM	HBG414030.
OMA	GQILDIR.
ProtClustDB	PRK01066.
Family and domain databases
HAMAP	MF_00260. Porphobil_deam. Divergent sequence. [Tree]
InterPro	IPR000860. 4pyrrol_synth_OHMeBilane_synth. IPR022417. Porphobilin_deaminase_N. [Graphical view]
KO	K01749.
PANTHER	PTHR11557. Porphobil_deam. 1 hit.
Pfam	PF01379. Porphobil_deam. 1 hit. [Graphical view]
PRINTS	PR00151. PORPHBDMNASE.
PROSITE	PS00533. PORPHOBILINOGEN_DEAM. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM3_CHLTR

Accession

Primary (citable) accession number: O84301

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program