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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi109IronUniRule annotation1
Metal bindingi113IronUniRule annotation1
Binding sitei165SubstrateUniRule annotation1
Binding sitei178Substrate; via amide nitrogenUniRule annotation1
Binding sitei277SubstrateUniRule annotation1
Metal bindingi302IronUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processtRNA processing
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciCTRA272561:G1G18-207-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:CT_197
OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
Taxonomic identifieri272561 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000431 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003033171 – 338tRNA N6-adenosine threonylcarbamoyltransferaseAdd BLAST338

Structurei

3D structure databases

ProteinModelPortaliO84200
SMRiO84200
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni132 – 136Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPM Bacteria
COG0533 LUCA
InParanoidiO84200
KOiK01409
OMAiHLEGHIY

Family and domain databases

HAMAPiMF_01445 TsaD, 1 hit
InterProiView protein in InterPro
IPR000905 Gcp-like_dom
IPR017861 KAE1/TsaD
IPR017860 Peptidase_M22_CS
IPR022450 TsaD
PANTHERiPTHR11735 PTHR11735, 1 hit
PTHR11735:SF6 PTHR11735:SF6, 1 hit
PfamiView protein in Pfam
PF00814 Peptidase_M22, 1 hit
PRINTSiPR00789 OSIALOPTASE
TIGRFAMsiTIGR00329 gcp_kae1, 1 hit
TIGR03723 T6A_TsaD_YgjD, 1 hit
PROSITEiView protein in PROSITE
PS01016 GLYCOPROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

O84200-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTLGLESSC DETSCSLVQN GKILANKIAS QDIHASYGGV IPELASRAHL
60 70 80 90 100
QTFPELLTAA TQSAGVSLED IELISVANTP GLIGALSIGV NFAKGLASGL
110 120 130 140 150
KRPLIGVNHV EAHLYAACME APATQFPALG LAISGAHTSL FLMPDATTFL
160 170 180 190 200
LIGKTRDDAI GETFDKVARF LGLPYPGGQK LEELAREGDA DAFAFSPARV
210 220 230 240 250
SGYDFSFSGL KTAVLYALKG NNSSAKAPFP EVSETQKRNI AASFQKAVFM
260 270 280 290 300
TIAQKLPDIV KTFSCESLIV GGGVANNSYF RRLLNQICSL PIYFPSSQLC
310 320 330
SDNAAMIAGL GERLFCNRTH VSKEVIPCAR YQWESACS
Length:338
Mass (Da):35,922
Last modified:November 1, 1998 - v1
Checksum:i2616704A31570E2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA Translation: AAC67789.1
PIRiA71545
RefSeqiNP_219701.1, NC_000117.1
WP_009871543.1, NC_000117.1

Genome annotation databases

EnsemblBacteriaiAAC67789; AAC67789; CT_197
GeneIDi35556400
884930
KEGGictr:CT_197
PATRICifig|272561.5.peg.211

Similar proteinsi

Entry informationi

Entry nameiTSAD_CHLTR
AccessioniPrimary (citable) accession number: O84200
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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