O84192 (GYRA_CHLTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	CT_189

Organism

Chlamydia trachomatis

Taxonomic identifier

813 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	836 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 836	836	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145230
Sites
	Active site	122	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O84192 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: AA750FA55138B5FC	FASTA	836	94,233
	10 20 30 40 50 60 MLNKEEIIVP KNLEEEMKES YLRYSMSVII SRALPDARDG LKPSQRRILY AMKQLNLTPG 70 80 90 100 110 120 VKHRKCAKIC GDTSGDYHPH GESVIYPTLV RMAQDWAMRY PLVDGQGNFG SIDGDPAAAM 130 140 150 160 170 180 RYTEARLTHS AIFLLEDLDK DTVDMVPNYD ETKYEPVVFP SKFPNLLCNG SSGIAVGMAT 190 200 210 220 230 240 NIPPHNLGEL IEATLLVLAN SQTSIEDILE VMPGPDFPTG GIICGTEGIR STYYTGRGKL 250 260 270 280 290 300 RLRARMHVEE NSDKQRENII LTEMPYNVNK SRLIEQIAEL INEKTLTGIS DVRDESDKDG 310 320 330 340 350 360 IRVVLELKKG ESSEVVINRL YKFTDVQVTF GANMLALDKN LPRTMNIHRM ISAWIRHRMD 370 380 390 400 410 420 VIQRRTRYEL NKAEARAHIL EGFLKALSCM DEVVKTIRES SNKEHAKQQL VELFSFSEAQ 430 440 450 460 470 480 ALAILELRLY QLTGLEADKV QKEYSELLEK ITYYRKVLAE EELVKDIIRE ELQELHKVHK 490 500 510 520 530 540 TPRRTRIEMD AGDVRDIEDI ISDESVIITI SGDDYVKRMP VKVFREQKRG GQGVTGFDMK 550 560 570 580 590 600 KGSDFLKAVY SASTKDYLLI FTNFGQCYWL KVWRLPEGER RAKGKPIINF LEGIRPGEQV 610 620 630 640 650 660 AAVLNVKRFE QGEYLFLATK KGVVKKVSLD AFGSPRKKGI RALEIDDGDE LIAARHIAND 670 680 690 700 710 720 EEKVMLFTRL GMAVRFPHDK VRPMGRAARG VRGVSLKNEQ DFVVSCQVVT EDQSVLVVCD 730 740 750 760 770 780 NGFGKRSLVC DFRETNRGSV GVRSIVINQR NGDVLGAISV TDCDSILLMS AQGQAIRINM 790 800 810 820 830 QDVRVMGRAT QGVRLVNLRE GDTLVAMEKL SINTESVETE ENLAASVQSG QDTIEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis." Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W. Science 282:754-759(1998) [PubMed: 9784136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D/UW-3/Cx.
[2]	"Sequencing of gyrase and topoisomerase IV QRDRs of Chlamydia trachomatis and characterization of quinolone-resistant mutants obtained in vitro." Dessus-Babus S., Bebear C.M., Charron A., Bebear C., de Barbeyrac B. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-179. Strain: L2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001273 Genomic DNA. Translation: AAC67781.1. AF044267 Genomic DNA. Translation: AAC33552.1.
PIR	F71546.
RefSeq	NP_219693.1. NC_000117.1.
3D structure databases
ProteinModelPortal	O84192.
ModBase	Search...
2D gel databases
PHCI-2DPAGE	O84192.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	884941.
GenomeReviews	Gene locus CT_189 in contig AE001273_GR.
KEGG	ctr:CT189.
PATRIC	20379881. VBIChlTra43535_0203.
Phylogenomic databases
HOGENOM	HBG635183.
OMA	TGRGRIY.
ProtClustDB	PRK05560.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_CHLTR

Accession

Primary (citable) accession number: O84192

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program