O84173 (TRPA_CHLTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan synthase alpha chain
EC=4.2.1.20

Gene names

Name:	trpA
Ordered Locus Names:	CT_171

Organism

Chlamydia trachomatis

Taxonomic identifier

813 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. HAMAP MF_00131
Catalytic activity	L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H2O. HAMAP MF_00131
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP MF_00131
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Sequence similarities	Belongs to the TrpA family.
Caution	This TrpA is highly divergent compared to other bacterial TrpA. As C.trachomatis seems to have lost part of the trp biosynthetic operon, it is possible that this protein is not active.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	tryptophan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Tryptophan synthase alpha chain HAMAP MF_00131		PRO_0000098768
Sites
	Active site	44	1	Proton acceptor By similarity
	Active site	55	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	O84173 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: D1A704445C630AEA	FASTA	253	28,056
	10 20 30 40 50 60 MSKLTQVFKQ TKLCIGYLTA GDGGTSYTIE AAKALIQGGV DILELGFPFS DPVADNPEIQ 70 80 90 100 110 120 VSHDRALAEN LTSETLLEIV EGIRAFNQEV PLILYSYYNP LLQRDLDYLR RLKDAGINGV 130 140 150 160 170 180 CVIDLPAPLS HGEKSPFFED LLAVGLDPIL LISAGTTPER MSLIQEYARG FLYYIPCQAT 190 200 210 220 230 240 RDSEVGIKEE FRKVREHFDL PIVDRRDICD KKEAAHVLNY SDGFIVKTAF VHQTTMDSSV 250 ETLTALAQTV IPG

References

[1]	"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis." Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W. Science 282:754-759(1998) [PubMed: 9784136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D/UW-3/Cx.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001273 Genomic DNA. Translation: AAC67762.1.
PIR	B71547.
RefSeq	NP_219674.1. NC_000117.1.
3D structure databases
ProteinModelPortal	O84173.
ModBase	Search...
2D gel databases
PHCI-2DPAGE	O84173.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	884962.
GenomeReviews	Gene locus CT_171 in contig AE001273_GR.
KEGG	ctr:CT171.
PATRIC	20379839. VBIChlTra43535_0184.
Phylogenomic databases
HOGENOM	HBG391690.
OMA	TSEARAD.
ProtClustDB	CLSK871230.
Family and domain databases
HAMAP	MF_00131. Trp_synth_alpha. Divergent sequence. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR011060. RibuloseP-bd_barrel. IPR018204. Trp_synthase_alpha_AS. IPR002028. Trp_synthase_suA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01695.
Pfam	PF00290. Trp_syntA. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00262. TrpA. 1 hit.
PROSITE	PS00167. TRP_SYNTHASE_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPA_CHLTR

Accession

Primary (citable) accession number: O84173

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program