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O84147 (PKN1_CHLTR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase pkn1

EC=2.7.11.1
Gene names
Name:pkn1
Ordered Locus Names:CT_145
OrganismChlamydia trachomatis (strain D/UW-3/Cx) [Reference proteome] [HAMAP]
Taxonomic identifier272561 [NCBI]
Taxonomic lineageBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia

Protein attributes

Sequence length614 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Together with the serine/threonine kinase PknD, may play a role in the specific interactions with host proteins during intracellular growth. Phosphorylates IncG, a inclusion-membrane protein required for the modification of the nascent chlamydial inclusion.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Developmental stage

Expressed at early mid-phase of the developmental cycle.

Post-translational modification

Autophosphorylated on serine and threonine residues. Ref.2

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Caution

Gln-136 is present instead of the conserved Asp which is expected to be an active site residue.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 614614Serine/threonine-protein kinase pkn1
PRO_0000171189

Regions

Domain13 – 276264Protein kinase
Nucleotide binding19 – 279ATP By similarity

Sites

Binding site421ATP By similarity

Experimental info

Mutagenesis421K → A: Loss of activity. Ref.2
Sequence conflict21E → D in AAN71625. Ref.2
Sequence conflict141K → R in AAN71625. Ref.2
Sequence conflict321H → R in AAN71625. Ref.2
Sequence conflict1051G → R in AAN71625. Ref.2
Sequence conflict2541V → A in AAN71625. Ref.2
Sequence conflict3291V → A in AAN71625. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O84147 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 28BEC559CD694001

FASTA61469,694
        10         20         30         40         50         60 
MEERAAVEYW GDYKVIAELG HGLWSRDVLA EHRFIKKRYI LKILPSELSS SENFMRVFQE 

        70         80         90        100        110        120 
VIVQLAAIRH ASLVAIENVS REGDRYFVVT EENGGTISLA QYLSGRKLSE EEVVHLIQQL 

       130        140        150        160        170        180 
CDALELVHSI GLAHGQIHLH SVHVSFFNGI ANIYLPEVGF ASLLRERMFS TIMQSGSARE 

       190        200        210        220        230        240 
SITRIRDLLM FEAPEEQEVF GREADVYSVG VLAYYLLVGS FPWGSFPKPS LCMPDSWYDW 

       250        260        270        280        290        300 
DGFILSCLQQ QREVRPKCLR EALRRKTSGE QLQVTLDSCR EPLREMEIED TPTELGPPSA 

       310        320        330        340        350        360 
LIREGERLCE VKEEQHAFVL VEAKSIDEVM VTTVDSEEEL ESSEGYANPL QSLLAREPVV 

       370        380        390        400        410        420 
SRYVEVEREE IKPQPLLTEM IFIEGGEFSR GSGDGQRDEL PVHNITLPGF FLDIHPVTNE 

       430        440        450        460        470        480 
QFVRFLECVG SEQDEHYNEL IRLKDSRIQR RSGRLIIEPG YAKHPVVGVT WYGASSYACW 

       490        500        510        520        530        540 
IGKRLPSEAE WEVAASGGKL GLRYPTGEEI DKSKANFFSS DTTPVMSYPS SILGLYDMAG 

       550        560        570        580        590        600 
NVYEWCQDWY SYDFYESSAL EPDAPLGPPQ GVYRVLRGGC WKSLKDDLRC AHRHRNNPGA 

       610 
INSTYGFRCA KDVK 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis."
Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.
Science 282:754-759(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: D/UW-3/Cx.
[2]"Identification of two eukaryote-like serine/threonine kinases encoded by Chlamydia trachomatis serovar L2 and characterization of interacting partners of Pkn1."
Verma A., Maurelli A.T.
Infect. Immun. 71:5772-5784(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, INTERACTION WITH PKND, PHOSPHORYLATION OF INCG, MUTAGENESIS OF LYS-42.
Strain: L2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001273 Genomic DNA. Translation: AAC67736.1.
AY148435 Genomic DNA. Translation: AAN71625.1.
PIRB71551.
RefSeqNP_219648.1. NC_000117.1.

3D structure databases

ProteinModelPortalO84147.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272561.CT145.

PTM databases

PhosSiteP0501121.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC67736; AAC67736; CT_145.
GeneID884193.
KEGGctr:CT_145.
PATRIC20379787. VBIChlTra43535_0158.

Phylogenomic databases

eggNOGCOG0515.
OMAFIRYLEC.
OrthoDBEOG622PMB.

Family and domain databases

Gene3D3.90.1580.10. 1 hit.
InterProIPR016187. C-type_lectin_fold.
IPR005532. FGE_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamPF03781. FGE-sulfatase. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
SSF56436. SSF56436. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePKN1_CHLTR
AccessionPrimary (citable) accession number: O84147
Secondary accession number(s): Q8GDH8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: November 1, 1998
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families