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Protein

Serine/threonine-protein kinase pkn1

Gene

pkn1

Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Together with the serine/threonine kinase PknD, may play a role in the specific interactions with host proteins during intracellular growth. Phosphorylates IncG, a inclusion-membrane protein required for the modification of the nascent chlamydial inclusion.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 279ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase pkn1 (EC:2.7.11.1)
Gene namesi
Name:pkn1
Ordered Locus Names:CT_145
OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
Taxonomic identifieri272561 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000431: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi42 – 421K → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 614614Serine/threonine-protein kinase pkn1PRO_0000171189Add
BLAST

Post-translational modificationi

Autophosphorylated on serine and threonine residues.1 Publication

Keywords - PTMi

Phosphoprotein

PTM databases

PhosSiteiP0501121.

Expressioni

Developmental stagei

Expressed at early mid-phase of the developmental cycle.

Interactioni

Protein-protein interaction databases

STRINGi272561.CT145.

Structurei

3D structure databases

ProteinModelPortaliO84147.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 276264Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiO84147.
OMAiFIRYLEC.
OrthoDBiEOG622PMB.

Family and domain databases

Gene3Di3.90.1580.10. 1 hit.
InterProiIPR016187. C-type_lectin_fold.
IPR005532. FGE_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF03781. FGE-sulfatase. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
SSF56436. SSF56436. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O84147-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEERAAVEYW GDYKVIAELG HGLWSRDVLA EHRFIKKRYI LKILPSELSS
60 70 80 90 100
SENFMRVFQE VIVQLAAIRH ASLVAIENVS REGDRYFVVT EENGGTISLA
110 120 130 140 150
QYLSGRKLSE EEVVHLIQQL CDALELVHSI GLAHGQIHLH SVHVSFFNGI
160 170 180 190 200
ANIYLPEVGF ASLLRERMFS TIMQSGSARE SITRIRDLLM FEAPEEQEVF
210 220 230 240 250
GREADVYSVG VLAYYLLVGS FPWGSFPKPS LCMPDSWYDW DGFILSCLQQ
260 270 280 290 300
QREVRPKCLR EALRRKTSGE QLQVTLDSCR EPLREMEIED TPTELGPPSA
310 320 330 340 350
LIREGERLCE VKEEQHAFVL VEAKSIDEVM VTTVDSEEEL ESSEGYANPL
360 370 380 390 400
QSLLAREPVV SRYVEVEREE IKPQPLLTEM IFIEGGEFSR GSGDGQRDEL
410 420 430 440 450
PVHNITLPGF FLDIHPVTNE QFVRFLECVG SEQDEHYNEL IRLKDSRIQR
460 470 480 490 500
RSGRLIIEPG YAKHPVVGVT WYGASSYACW IGKRLPSEAE WEVAASGGKL
510 520 530 540 550
GLRYPTGEEI DKSKANFFSS DTTPVMSYPS SILGLYDMAG NVYEWCQDWY
560 570 580 590 600
SYDFYESSAL EPDAPLGPPQ GVYRVLRGGC WKSLKDDLRC AHRHRNNPGA
610
INSTYGFRCA KDVK
Length:614
Mass (Da):69,694
Last modified:November 1, 1998 - v1
Checksum:i28BEC559CD694001
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21E → D in AAN71625. (PubMed:14500499)Curated
Sequence conflicti14 – 141K → R in AAN71625. (PubMed:14500499)Curated
Sequence conflicti32 – 321H → R in AAN71625. (PubMed:14500499)Curated
Sequence conflicti105 – 1051G → R in AAN71625. (PubMed:14500499)Curated
Sequence conflicti254 – 2541V → A in AAN71625. (PubMed:14500499)Curated
Sequence conflicti329 – 3291V → A in AAN71625. (PubMed:14500499)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC67736.1.
AY148435 Genomic DNA. Translation: AAN71625.1.
PIRiB71551.
RefSeqiNP_219648.1. NC_000117.1.

Genome annotation databases

EnsemblBacteriaiAAC67736; AAC67736; CT_145.
GeneIDi884193.
KEGGictr:CT_145.
PATRICi20379787. VBIChlTra43535_0158.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC67736.1.
AY148435 Genomic DNA. Translation: AAN71625.1.
PIRiB71551.
RefSeqiNP_219648.1. NC_000117.1.

3D structure databases

ProteinModelPortaliO84147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272561.CT145.

PTM databases

PhosSiteiP0501121.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC67736; AAC67736; CT_145.
GeneIDi884193.
KEGGictr:CT_145.
PATRICi20379787. VBIChlTra43535_0158.

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiO84147.
OMAiFIRYLEC.
OrthoDBiEOG622PMB.

Family and domain databases

Gene3Di3.90.1580.10. 1 hit.
InterProiIPR016187. C-type_lectin_fold.
IPR005532. FGE_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF03781. FGE-sulfatase. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
SSF56436. SSF56436. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis."
    Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.
    Science 282:754-759(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: D/UW-3/Cx.
  2. "Identification of two eukaryote-like serine/threonine kinases encoded by Chlamydia trachomatis serovar L2 and characterization of interacting partners of Pkn1."
    Verma A., Maurelli A.T.
    Infect. Immun. 71:5772-5784(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, INTERACTION WITH PKND, PHOSPHORYLATION OF INCG, MUTAGENESIS OF LYS-42.
    Strain: L2.

Entry informationi

Entry nameiPKN1_CHLTR
AccessioniPrimary (citable) accession number: O84147
Secondary accession number(s): Q8GDH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: November 1, 1998
Last modified: January 7, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Gln-136 is present instead of the conserved Asp which is expected to be an active site residue.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.