O84147 (PKN1_CHLTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase pkn1
EC=2.7.11.1

Gene names

Name:	pkn1
Ordered Locus Names:	CT_145

Organism

Chlamydia trachomatis (strain D/UW-3/Cx) [Reference proteome] [HAMAP]

Taxonomic identifier

272561 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chlamydiae/Verrucomicrobia group › Chlamydiae › Chlamydiia › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia › Chlamydia trachomatis

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Together with the serine/threonine kinase PknD, may play a role in the specific interactions with host proteins during intracellular growth. Phosphorylates IncG, a inclusion-membrane protein required for the modification of the nascent chlamydial inclusion.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Developmental stage	Expressed at early mid-phase of the developmental cycle.
Post-translational modification	Autophosphorylated on serine and threonine residues. Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Caution	Gln-136 is present instead of the conserved Asp which is expected to be an active site residue.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 614	614	Serine/threonine-protein kinase pkn1		PRO_0000171189
Regions
	Domain	13 – 276	264	Protein kinase
	Nucleotide binding	19 – 27	9	ATP By similarity
Sites
	Binding site	42	1	ATP By similarity
Experimental info
	Mutagenesis	42	1	K → A: Loss of activity. Ref.2
	Sequence conflict	2	1	E → D in AAN71625. Ref.2
	Sequence conflict	14	1	K → R in AAN71625. Ref.2
	Sequence conflict	32	1	H → R in AAN71625. Ref.2
	Sequence conflict	105	1	G → R in AAN71625. Ref.2
	Sequence conflict	254	1	V → A in AAN71625. Ref.2
	Sequence conflict	329	1	V → A in AAN71625. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O84147 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 28BEC559CD694001	FASTA	614	69,694
	10 20 30 40 50 60 MEERAAVEYW GDYKVIAELG HGLWSRDVLA EHRFIKKRYI LKILPSELSS SENFMRVFQE 70 80 90 100 110 120 VIVQLAAIRH ASLVAIENVS REGDRYFVVT EENGGTISLA QYLSGRKLSE EEVVHLIQQL 130 140 150 160 170 180 CDALELVHSI GLAHGQIHLH SVHVSFFNGI ANIYLPEVGF ASLLRERMFS TIMQSGSARE 190 200 210 220 230 240 SITRIRDLLM FEAPEEQEVF GREADVYSVG VLAYYLLVGS FPWGSFPKPS LCMPDSWYDW 250 260 270 280 290 300 DGFILSCLQQ QREVRPKCLR EALRRKTSGE QLQVTLDSCR EPLREMEIED TPTELGPPSA 310 320 330 340 350 360 LIREGERLCE VKEEQHAFVL VEAKSIDEVM VTTVDSEEEL ESSEGYANPL QSLLAREPVV 370 380 390 400 410 420 SRYVEVEREE IKPQPLLTEM IFIEGGEFSR GSGDGQRDEL PVHNITLPGF FLDIHPVTNE 430 440 450 460 470 480 QFVRFLECVG SEQDEHYNEL IRLKDSRIQR RSGRLIIEPG YAKHPVVGVT WYGASSYACW 490 500 510 520 530 540 IGKRLPSEAE WEVAASGGKL GLRYPTGEEI DKSKANFFSS DTTPVMSYPS SILGLYDMAG 550 560 570 580 590 600 NVYEWCQDWY SYDFYESSAL EPDAPLGPPQ GVYRVLRGGC WKSLKDDLRC AHRHRNNPGA 610 INSTYGFRCA KDVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis." Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W. Science 282:754-759(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D/UW-3/Cx.
[2]	"Identification of two eukaryote-like serine/threonine kinases encoded by Chlamydia trachomatis serovar L2 and characterization of interacting partners of Pkn1." Verma A., Maurelli A.T. Infect. Immun. 71:5772-5784(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, INTERACTION WITH PKND, PHOSPHORYLATION OF INCG, MUTAGENESIS OF LYS-42. Strain: L2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001273 Genomic DNA. Translation: AAC67736.1. AY148435 Genomic DNA. Translation: AAN71625.1.
PIR	B71551.
RefSeq	NP_219648.1. NC_000117.1.
3D structure databases
ProteinModelPortal	O84147.
ModBase	Search...
Protein-protein interaction databases
STRING	272561.CT145.
PTM databases
PhosSite	P0501121.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC67736; AAC67736; CT_145.
GeneID	884193.
KEGG	ctr:CT145.
PATRIC	20379787. VBIChlTra43535_0158.
Phylogenomic databases
eggNOG	COG0515.
OMA	EGYVNPL.
ProtClustDB	CLSK871217.
Family and domain databases
Gene3D	3.90.1580.10. 1 hit.
InterPro	IPR016187. C-type_lectin_fold. IPR005532. FGE_dom. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. [Graphical view]
Pfam	PF03781. FGE-sulfatase. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS50011. PROTEIN_KINASE_DOM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PKN1_CHLTR

Accession

Primary (citable) accession number: O84147
Secondary accession number(s): Q8GDH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program