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Protein

Chaperone protein ClpB

Gene

clpB

Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi205 – 2128ATP 1By similarity
Nucleotide bindingi605 – 6128ATP 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB
Gene namesi
Name:clpB
Ordered Locus Names:CT_113
OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
Taxonomic identifieri272561 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000431 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 867867Chaperone protein ClpBPRO_0000191109Add
BLAST

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

Protein-protein interaction databases

STRINGi272561.CT113.

Structurei

3D structure databases

ProteinModelPortaliO84115.
SMRiO84115. Positions 156-350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 142142N-terminalBy similarityAdd
BLAST
Regioni158 – 339182NBD1By similarityAdd
BLAST
Regioni340 – 545206LinkerBy similarityAdd
BLAST
Regioni555 – 769215NBD2By similarityAdd
BLAST
Regioni770 – 86798C-terminalBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili390 – 524135By similarityAdd
BLAST

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
InParanoidiO84115.
KOiK03695.
OMAiDMGIELS.
OrthoDBiEOG65F8SM.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O84115-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKFSDAVSE ALEKAFELAK NSKHSYVTEN HLLKSLLQNP GSLFCLVIKD
60 70 80 90 100
VHGNLGLLTS AVDDALRREP TVVEGTAVAS PSPSLQQLLL NAHQEARSMG
110 120 130 140 150
DEYLSGDHLL LAFWRSTKEP FASWRKTVKT TSEALKELIT KLRQGSRMDS
160 170 180 190 200
PSAEENLKGL EKYCKNLTVL AREGKLDPVI GRDEEIRRTI QVLSRRTKNN
210 220 230 240 250
PMLIGEPGVG KTAIAEGLAL RIVQGDVPES LKEKHLYVLD MGALIAGAKY
260 270 280 290 300
RGEFEERLKS VLKGVEASEG ECILFIDEVH TLVGAGATDG AMDAANLLKP
310 320 330 340 350
ALARGTLHCI GATTLNEYQK YIEKDAALER RFQPIFVTEP SLEDAVFILR
360 370 380 390 400
GLREKYEIFH GVRITEGALN AAVVLSYRYI TDRFLPDKAI DLIDEAASLI
410 420 430 440 450
RMQIGSLPLP IDEKERELSA LIVKQEAIKR EQAPAYQEEA EDMQKAIDRV
460 470 480 490 500
KEELAALRLR WDEEKGLITG LKEKKNALEN LKFAEEEAER TADYNRVAEL
510 520 530 540 550
RYSLIPSLEE EIHLAEEALN QRDGRLLQEE VDERLIAQVV ANWTGIPVQK
560 570 580 590 600
MLEGESEKLL VLEESLEERV VGQPFAIAAV SDSIRAARVG LSDPQRPLGV
610 620 630 640 650
FLFLGPTGVG KTELAKALAE LLFNKEEAMI RFDMTEYMEK HSVSKLIGSP
660 670 680 690 700
PGYVGYEEGG SLSEALRRRP YSVVLFDEIE KADKEVFNIL LQIFDDGILT
710 720 730 740 750
DSKKRKVNCK NALFIMTSNI GSQELADYCT KKGTIVDKEA VLSVVAPALK
760 770 780 790 800
NYFSPEFINR IDDILPFVPL TTEDIVKIVG IQMNRVALRL LERKISLTWD
810 820 830 840 850
DSLVLFLSEQ GYDSAFGARP LKRLIQQKVV TMLSKALLKG DIKPGMAVEL
860
TMAKDVVVFK IKTNPAV
Length:867
Mass (Da):96,630
Last modified:November 1, 1998 - v1
Checksum:i4B5A98F587BA7BAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC67704.1.
PIRiD71555.
RefSeqiNP_219616.1. NC_000117.1.
WP_010725067.1. NC_000117.1.

Genome annotation databases

EnsemblBacteriaiAAC67704; AAC67704; CT_113.
GeneIDi884031.
KEGGictr:CT_113.
PATRICi20379715. VBIChlTra43535_0124.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC67704.1.
PIRiD71555.
RefSeqiNP_219616.1. NC_000117.1.
WP_010725067.1. NC_000117.1.

3D structure databases

ProteinModelPortaliO84115.
SMRiO84115. Positions 156-350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272561.CT113.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC67704; AAC67704; CT_113.
GeneIDi884031.
KEGGictr:CT_113.
PATRICi20379715. VBIChlTra43535_0124.

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
InParanoidiO84115.
KOiK03695.
OMAiDMGIELS.
OrthoDBiEOG65F8SM.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis."
    Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.
    Science 282:754-759(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: D/UW-3/Cx.

Entry informationi

Entry nameiCLPB_CHLTR
AccessioniPrimary (citable) accession number: O84115
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: November 1, 1998
Last modified: January 20, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.