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Protein

Elongation factor 4

Gene

lepA

Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 246GTPUniRule annotation
Nucleotide bindingi135 – 1384GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:CT_064
OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
Taxonomic identifieri272561 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000431 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 602602Elongation factor 4PRO_0000176259Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272561.CT064.

Structurei

3D structure databases

ProteinModelPortaliO84067.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 188182tr-type GAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4S. Bacteria.
COG0481. LUCA.
InParanoidiO84067.
KOiK03596.
OMAiKPMVFCG.
OrthoDBiEOG6ZKXQ4.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O84067-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPYKIENIR NFSIIAHIDH GKSTIADRLL ESTSTIEQRE MREQLLDSMD
60 70 80 90 100
LERERGITIK AHPVTMTYEY EGETYELNLI DTPGHVDFSY EVSRSLAACE
110 120 130 140 150
GALLIVDAAQ GVQAQSLANV YLALERDLEI IPVLNKIDLP AAQPEAIKKQ
160 170 180 190 200
IEEFIGLDTS NTIACSAKTG QGIPEILESI IRLVPPPKPP QETELKALIF
210 220 230 240 250
DSHYDPYVGI MVYVRVISGE IKKGDRITFM ATKGSSFEVL GIGAFLPEAT
260 270 280 290 300
LMEGSLRAGQ VGYFIANLKK VKDVKIGDTV TTVKHPAKEP LEGFKEIKPV
310 320 330 340 350
VFAGIYPIDS SDFDTLKDAL GRLQLNDSAL TIEQENSHSL GFGFRCGFLG
360 370 380 390 400
LLHLEIIFER ISREFDLDII ATAPSVIYKV VLKNGKTLFI DNPTAYPDPA
410 420 430 440 450
LIEHMEEPWV HVNIITPQEY LSNIMSLCMD KRGICLKTDM LDQHRLVLSY
460 470 480 490 500
ELPLNEIVSD FNDKLKSVTK GYGSFDYRLG DYKKGAIIKL EILINDEAVD
510 520 530 540 550
AFSCLVHRDK AESKGRSICE KLVDVIPPQL FKIPIQAAIN KKIIARETIR
560 570 580 590 600
ALAKNVTAKC YGGDITRKRK LWDKQKKGKK RMKEFGKVSI PNTAFVEVLK

ME
Length:602
Mass (Da):67,435
Last modified:November 1, 1998 - v1
Checksum:i98F67C990196EEC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC67655.1.
PIRiB71561.
RefSeqiNP_219567.1. NC_000117.1.
WP_009872356.1. NC_000117.1.

Genome annotation databases

EnsemblBacteriaiAAC67655; AAC67655; CT_064.
GeneIDi884077.
KEGGictr:CT_064.
PATRICi20379613. VBIChlTra43535_0073.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC67655.1.
PIRiB71561.
RefSeqiNP_219567.1. NC_000117.1.
WP_009872356.1. NC_000117.1.

3D structure databases

ProteinModelPortaliO84067.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272561.CT064.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC67655; AAC67655; CT_064.
GeneIDi884077.
KEGGictr:CT_064.
PATRICi20379613. VBIChlTra43535_0073.

Phylogenomic databases

eggNOGiENOG4105C4S. Bacteria.
COG0481. LUCA.
InParanoidiO84067.
KOiK03596.
OMAiKPMVFCG.
OrthoDBiEOG6ZKXQ4.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis."
    Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.
    Science 282:754-759(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: D/UW-3/Cx.

Entry informationi

Entry nameiLEPA_CHLTR
AccessioniPrimary (citable) accession number: O84067
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.