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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.By similarity

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi145 – 1451ZincBy similarity
Metal bindingi148 – 1481ZincBy similarity
Metal bindingi158 – 1581ZincBy similarity
Metal bindingi161 – 1611ZincBy similarity
Binding sitei334 – 3341ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:metG
Ordered Locus Names:CT_032
OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
Taxonomic identifieri272561 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000431 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 550550Methionine--tRNA ligasePRO_0000139123Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi272561.CT032.

Structurei

3D structure databases

ProteinModelPortaliO84035.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi13 – 2311"HIGH" regionAdd
BLAST
Motifi331 – 3355"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0143. LUCA.
InParanoidiO84035.
KOiK01874.
OMAiCHEAARG.
OrthoDBiEOG6CVV9B.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1.
InterProiIPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.

Sequencei

Sequence statusi: Complete.

O84035-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESSRILITS ALPYANGPLH FGHITGAYLP ADVYARFQRL QGKEVLYICG
60 70 80 90 100
SDEYGIAITL NAELAGMGYQ EYVDMYHKLH KDTFKKLGIS VDFFSRTTNT
110 120 130 140 150
YHPAIVQDFY RNLQERGLVE NQVTEQLYSE EEGKFLADRY VVGTCPKCGF
160 170 180 190 200
DRARGDECQQ CGADYEARDL KEPRSKLTGA ALSLRDTEHA YLHLERMKED
210 220 230 240 250
LLAFVQGIYL RPHMRNFVTD YIEHLRPRAV TRDLSWGIPV PDLENKVFYV
260 270 280 290 300
WFDAPIGYIS GTMDWAASIG DPEAWKKFWL DDTVTYAQFI GKDNTSFHAA
310 320 330 340 350
IFPAMEIGQS LPYKKVDALV TSEFLLLEGF QFSKSDGNFI DMDAFLETYS
360 370 380 390 400
LDKLRYVLAA IAPETSDSEF SFQEFKTRCN SELVGKYGNF VNRVLAFAVK
410 420 430 440 450
NGCTELSSPQ LEQKDLDFIS KSQKLAKDAA EHYAQYSLRK ACSTIMELAA
460 470 480 490 500
LGNGYFNDEA PWKLAKEGNW NRVRAILFCA CYCQKLLALI SYPIMPETAL
510 520 530 540 550
KILEMIAPHS LDLGSQDPDR LQSLWTDSFF DYSEEKFSLK EPELLFTMVE
Length:550
Mass (Da):62,716
Last modified:November 1, 1998 - v1
Checksum:iB829F13C23D5E9C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC67622.1.
PIRiC71567.
RefSeqiNP_219534.1. NC_000117.1.
WP_010724990.1. NC_000117.1.

Genome annotation databases

EnsemblBacteriaiAAC67622; AAC67622; CT_032.
GeneIDi884148.
KEGGictr:CT_032.
PATRICi20379537. VBIChlTra43535_0037.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC67622.1.
PIRiC71567.
RefSeqiNP_219534.1. NC_000117.1.
WP_010724990.1. NC_000117.1.

3D structure databases

ProteinModelPortaliO84035.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272561.CT032.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC67622; AAC67622; CT_032.
GeneIDi884148.
KEGGictr:CT_032.
PATRICi20379537. VBIChlTra43535_0037.

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0143. LUCA.
InParanoidiO84035.
KOiK01874.
OMAiCHEAARG.
OrthoDBiEOG6CVV9B.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1.
InterProiIPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis."
    Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.
    Science 282:754-759(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: D/UW-3/Cx.

Entry informationi

Entry nameiSYM_CHLTR
AccessioniPrimary (citable) accession number: O84035
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: July 6, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.