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O84022

- SYI_CHLTR

UniProt

O84022 - SYI_CHLTR

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei592 – 5921ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:CT_019
    OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
    Taxonomic identifieri272561 [NCBI]
    Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
    ProteomesiUP000000431: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10361036Isoleucine--tRNA ligasePRO_0000098529Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272561.CT019.

    Structurei

    3D structure databases

    ProteinModelPortaliO84022.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi46 – 5611"HIGH" regionAdd
    BLAST
    Motifi589 – 5935"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiENRDWFE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O84022-1 [UniParc]FASTAAdd to Basket

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    MDNEDKISIS AKEEKILSFW KEQDIFQKTL DNREGCPTFS FYDGPPFATG     50
    LPHYGHLLAG TIKDVVCRYA SMDGHYVPRR FGWDCHGVPV EYEVEKSLGL 100
    TEPGAIERFG VANFNEECRK IVFRYADEWK YFVDRIGRWV DFSATWRTMD 150
    LSFMESVWWV FRSLYDQGLV YEGTKVVPFS TKLGTPLSNF EAGQNYKEVD 200
    DPSVVVKFAL QDNQGFLLAW TTTPWTLVSN MALAVHPELT YVRIKDKESG 250
    DEYILGQESL PRWFPDRESY EWIGQLSGKS LVGQSYEPLF PYFQDKKELE 300
    AFRILPADFI EESEGTGIVH MAPAFGEADF FACQEHNVPL VCPVDNQGCY 350
    TAEVKDFVGE YIKSADKGIA RRLKNENKLF YQGTVRHRYP FCWRTDSPLI 400
    YKAVNSWFVA VEKVKSKMLK ANESIHWTPE HIKQGRFGKW LEGARDWAIS 450
    RNRYWGTPIP IWRSDDGELL VIGSIQELEA LSGQKIVDLH RHFIDEIEIN 500
    QNGKSFRRIP YVFDCWFDSG AMPYAQNHYP FERAEETEAC FPADFIAEGL 550
    DQTRGWFYTL TVIAAALFDQ PAFKNVIVNG IILAEDGNKM SKRLNNYPSP 600
    KMIMDAYGAD ALRLYLLNSV VVKAEDLRFS DKGVESVLKQ VLLPLSNALA 650
    FYKTYAELYG FDPKETDNIE LAEIDRWILS SLYSLVGKTR ESMSQYDLHA 700
    AVNPFVDFIE DLTNWYIRRS RRRFWDAEDS ADRRAAFSTL YEVLVVFSKV 750
    IAPFIPFIAE DMYQQLRGET DPESVHLCDF PHVVLEKILP DLERKMQDIR 800
    EIVALGHSLR KEHKLKVRQP LQNVYIVGSK ERKEALAQVG SLIGEELNVK 850
    DVHFCSETPE YVTTLIKPNF RTLGKKVGNR LPEIQRALAG LPQEQIQAFM 900
    HKGQMVVSLG EETISLDKED ITVSWASAEG FVARSSASFV AVLDCQLTEP 950
    LIMEGIAREL VNKINTMRRN RKLHVSDRIA IRLHAPVIVQ EAFALHKEYI 1000
    CEETLTTSVS VIDYKEGEEW DINGHAVSFV LERVER 1036
    Length:1,036
    Mass (Da):118,985
    Last modified:November 1, 1998 - v1
    Checksum:iDC4420FD37343EFD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE001273 Genomic DNA. Translation: AAC67609.1.
    PIRiF71565.
    RefSeqiNP_219521.1. NC_000117.1.

    Genome annotation databases

    EnsemblBacteriaiAAC67609; AAC67609; CT_019.
    GeneIDi884097.
    KEGGictr:CT_019.
    PATRICi20379511. VBIChlTra43535_0024.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE001273 Genomic DNA. Translation: AAC67609.1 .
    PIRi F71565.
    RefSeqi NP_219521.1. NC_000117.1.

    3D structure databases

    ProteinModelPortali O84022.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272561.CT019.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC67609 ; AAC67609 ; CT_019 .
    GeneIDi 884097.
    KEGGi ctr:CT_019.
    PATRICi 20379511. VBIChlTra43535_0024.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi ENRDWFE.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis."
      Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.
      Science 282:754-759(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: D/UW-3/Cx.

    Entry informationi

    Entry nameiSYI_CHLTR
    AccessioniPrimary (citable) accession number: O84022
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3