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O84022

- SYI_CHLTR

UniProt

O84022 - SYI_CHLTR

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Protein
Isoleucine--tRNA ligase
Gene
ileS, CT_019
Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei592 – 5921ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:CT_019
OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
Taxonomic identifieri272561 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000431: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098529Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272561.CT019.

Structurei

3D structure databases

ProteinModelPortaliO84022.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 5611"HIGH" regionUniRule annotation
Add
BLAST
Motifi589 – 5935"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O84022-1 [UniParc]FASTAAdd to Basket

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MDNEDKISIS AKEEKILSFW KEQDIFQKTL DNREGCPTFS FYDGPPFATG     50
LPHYGHLLAG TIKDVVCRYA SMDGHYVPRR FGWDCHGVPV EYEVEKSLGL 100
TEPGAIERFG VANFNEECRK IVFRYADEWK YFVDRIGRWV DFSATWRTMD 150
LSFMESVWWV FRSLYDQGLV YEGTKVVPFS TKLGTPLSNF EAGQNYKEVD 200
DPSVVVKFAL QDNQGFLLAW TTTPWTLVSN MALAVHPELT YVRIKDKESG 250
DEYILGQESL PRWFPDRESY EWIGQLSGKS LVGQSYEPLF PYFQDKKELE 300
AFRILPADFI EESEGTGIVH MAPAFGEADF FACQEHNVPL VCPVDNQGCY 350
TAEVKDFVGE YIKSADKGIA RRLKNENKLF YQGTVRHRYP FCWRTDSPLI 400
YKAVNSWFVA VEKVKSKMLK ANESIHWTPE HIKQGRFGKW LEGARDWAIS 450
RNRYWGTPIP IWRSDDGELL VIGSIQELEA LSGQKIVDLH RHFIDEIEIN 500
QNGKSFRRIP YVFDCWFDSG AMPYAQNHYP FERAEETEAC FPADFIAEGL 550
DQTRGWFYTL TVIAAALFDQ PAFKNVIVNG IILAEDGNKM SKRLNNYPSP 600
KMIMDAYGAD ALRLYLLNSV VVKAEDLRFS DKGVESVLKQ VLLPLSNALA 650
FYKTYAELYG FDPKETDNIE LAEIDRWILS SLYSLVGKTR ESMSQYDLHA 700
AVNPFVDFIE DLTNWYIRRS RRRFWDAEDS ADRRAAFSTL YEVLVVFSKV 750
IAPFIPFIAE DMYQQLRGET DPESVHLCDF PHVVLEKILP DLERKMQDIR 800
EIVALGHSLR KEHKLKVRQP LQNVYIVGSK ERKEALAQVG SLIGEELNVK 850
DVHFCSETPE YVTTLIKPNF RTLGKKVGNR LPEIQRALAG LPQEQIQAFM 900
HKGQMVVSLG EETISLDKED ITVSWASAEG FVARSSASFV AVLDCQLTEP 950
LIMEGIAREL VNKINTMRRN RKLHVSDRIA IRLHAPVIVQ EAFALHKEYI 1000
CEETLTTSVS VIDYKEGEEW DINGHAVSFV LERVER 1036
Length:1,036
Mass (Da):118,985
Last modified:November 1, 1998 - v1
Checksum:iDC4420FD37343EFD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001273 Genomic DNA. Translation: AAC67609.1.
PIRiF71565.
RefSeqiNP_219521.1. NC_000117.1.

Genome annotation databases

EnsemblBacteriaiAAC67609; AAC67609; CT_019.
GeneIDi884097.
KEGGictr:CT_019.
PATRICi20379511. VBIChlTra43535_0024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001273 Genomic DNA. Translation: AAC67609.1 .
PIRi F71565.
RefSeqi NP_219521.1. NC_000117.1.

3D structure databases

ProteinModelPortali O84022.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272561.CT019.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC67609 ; AAC67609 ; CT_019 .
GeneIDi 884097.
KEGGi ctr:CT_019.
PATRICi 20379511. VBIChlTra43535_0024.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis."
    Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.
    Science 282:754-759(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: D/UW-3/Cx.

Entry informationi

Entry nameiSYI_CHLTR
AccessioniPrimary (citable) accession number: O84022
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: May 14, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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