O84007 (GATB_CHLTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-

Gene names

Name:	gatB
Ordered Locus Names:	CT_004

Organism

Chlamydia trachomatis

Taxonomic identifier

813 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP MF_00121
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP MF_00121 ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP MF_00121
Sequence similarities	Belongs to the GatB/GatE family. GatB subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-nitrogen ligase activity, with glutamine as amido-N-donor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP MF_00121		PRO_0000148779

Sequences

	Sequence		Length	Mass (Da)
	O84007 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: A6F9D586042DA112	FASTA	488	55,018
	10 20 30 40 50 60 MGIAHTEWES VIGLEVHVEL NTESKLFSPA RNHFGDEPNT NISPVCTGMP GSLPVLNKDA 70 80 90 100 110 120 VRKAVLFGCA VEGDVALFSR FDRKSYFYPD SPRNFQITQY EHPIVRGGCI RAVVEGEEKT 130 140 150 160 170 180 FELAQTHLED DAGMLKHFGD FAGVDYNRAG VPLIEIVSKP CMFSAEDAVA YANALVSILG 190 200 210 220 230 240 YIGISDCNME EGSIRFDVNI SVRPRGSREL RNKVEIKNMN SFTFMAQALE AEKRRQIEEY 250 260 270 280 290 300 LSYPNEDPKK VVPAATYRWD PEKKKTVLMR LKERAEDYMY FVEPDLPVLQ ITETYIDEVR 310 320 330 340 350 360 QTLPELPHSK YMRYITDFDI AEDLAMILVG DRHTAHFFET ATMSCKNYRA LSNWITVEFA 370 380 390 400 410 420 GRCKARGKTL PFTGILPEWV AQLVNFIDRG VITGKIAKEI ADRMVSSFGE SPEDILRRHP 430 440 450 460 470 480 SLLPMTDDHA LRAIVKEVVA QNTASVADYK NGKAKALGFL VGQIMKRTEG KAPPKRVNEL LLAAMRDM

References

[1]

"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis." Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W. Science 282:754-759(1998) [PubMed: 9784136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D/UW-3/Cx.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001273 Genomic DNA. Translation: AAC67594.1.
PIR	G71568.
RefSeq	NP_219506.1. NC_000117.1.
3D structure databases
ProteinModelPortal	O84007.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	884084.
GenomeReviews	Gene locus CT_004 in contig AE001273_GR.
KEGG	ctr:CT004.
PATRIC	20379473. VBIChlTra43535_0005.
Phylogenomic databases
HOGENOM	HBG395951.
OMA	KNYFYAD.
ProtClustDB	PRK05477.
Family and domain databases
HAMAP	MF_00121. GatB. [Tree]
InterPro	IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR023168. GatB_Yqey_C. IPR004413. Gln-tRNA_amidoTrfase_bsu. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
Gene3D	G3DSA:1.10.10.410. GatB_Yqey_C. 1 hit.
KO	K02434.
PANTHER	PTHR11659. GatB. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. GatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATB_CHLTR

Accession

Primary (citable) accession number: O84007

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program