O84006 (GATA_CHLTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A
Short name=Glu-ADT subunit A
EC=6.3.5.-

Gene names

Name:	gatA
Ordered Locus Names:	CT_003

Organism

Chlamydia trachomatis

Taxonomic identifier

813 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP MF_00120
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP MF_00120
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP MF_00120
Sequence similarities	Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-nitrogen ligase activity, with glutamine as amido-N-donor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP MF_00120		PRO_0000105153
Sites
	Active site	77	1	Charge relay system By similarity
	Active site	152	1	Charge relay system By similarity
	Active site	176	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O84006 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 00773EB3B3F6BDF2	FASTA	491	53,588
	10 20 30 40 50 60 MYRKSALELR DAVVNRELSV TAITEYFYHR IESHDEQIGA FLSLCKERAL LRASRIDDKL 70 80 90 100 110 120 AKGDPIGLLA GIPIGVKDNI HITGVKTTCA SKMLENFVAP FDSTVVRRIE MEDGILLGKL 130 140 150 160 170 180 NMDEFAMGST TRYSAFHPTN NPWDLERVPG GSSGGSAAAV SARFCPIALG SDTGGSIRQP 190 200 210 220 230 240 AAFCGVVGFK PSYGAVSRYG LVAFGSSLDQ IGPLTTVVED VALAMDAFAG RDPKDSTTRD 250 260 270 280 290 300 FFKGTFSQAL SLEVPKLIGV PRGFLDGLQE DCKENFFEAL AVMEREGSRI IDVDLSVLKH 310 320 330 340 350 360 AVPVYYIVAS AEAATNLARF DGVRYGHRCA QADNMHEMYA RSRKEGFGKE VTRRILLGNY 370 380 390 400 410 420 VLSAERQNIF YKKGMAVRAR LIDAFQAAFE RCDVIAMPVC ATPAIRDQDV LDPVSLYLQD 430 440 450 460 470 480 VYTVAVNLAY LPAISVPSGL SKEGLPLGVQ FIGERGSDQQ ICQVGYSFQE HSQIKQLYPK 490 AVNGLFDGGI E

References

[1]

"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis." Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W. Science 282:754-759(1998) [PubMed: 9784136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D/UW-3/Cx.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001273 Genomic DNA. Translation: AAC67593.1.
PIR	F71568.
RefSeq	NP_219505.1. NC_000117.1.
3D structure databases
ProteinModelPortal	O84006.
ModBase	Search...
2D gel databases
PHCI-2DPAGE	O84006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	884087.
GenomeReviews	Gene locus CT_003 in contig AE001273_GR.
KEGG	ctr:CT003.
PATRIC	20379471. VBIChlTra43535_0004.
Phylogenomic databases
HOGENOM	HBG481888.
OMA	FDEETLF.
PhylomeDB	O84006.
ProtClustDB	PRK00012.
Family and domain databases
HAMAP	MF_00120. GatA. [Tree]
InterPro	IPR000120. Amidase. IPR020556. Amidase_CS. IPR023631. Amidase_dom. IPR004412. GatA. [Graphical view]
Gene3D	G3DSA:3.90.1300.10. Amidase_sig_enz. 1 hit.
KO	K02433.
PANTHER	PTHR11895. Amidase. 1 hit.
Pfam	PF01425. Amidase. 1 hit. [Graphical view]
SUPFAM	SSF75304. Amidase_sig_enz. 1 hit.
TIGRFAMs	TIGR00132. GatA. 1 hit.
PROSITE	PS00571. AMIDASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATA_CHLTR

Accession

Primary (citable) accession number: O84006

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program