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O83998 (SYV_TREPA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Valine--tRNA ligase
EC=6.1.1.9
Alternative name(s):
Valyl-tRNA synthetase
Short name=ValRS
Gene names
Name:valS
Ordered Locus Names:TP_1035
OrganismTreponema pallidum (strain Nichols) [Complete proteome] [HAMAP]
Taxonomic identifier243276 [NCBI]
Taxonomic lineageBacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Treponema ›

Protein attributes

Sequence length956 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity. HAMAP-Rule MF_02004

Catalytic activity

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP-Rule MF_02004

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02004

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02004.

Domain

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. HAMAP-Rule MF_02004

The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity. HAMAP-Rule MF_02004

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 956956Valine--tRNA ligase HAMAP-Rule MF_02004
PRO_0000106241

Regions

Coiled coil885 – 91127 Potential
Motif69 – 7911"HIGH" region HAMAP-Rule MF_02004
Motif566 – 5705"KMSKS" region HAMAP-Rule MF_02004

Sites

Binding site5691ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
O83998 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 7D50583C7C6BA35A

FASTA956107,363
        10         20         30         40         50         60 
MTQKLQKIVL PPVYGPADFE ARVYACWEQR QAFSPRARGS GTSDSEGCDG HSRQIEGGAR 

        70         80         90        100        110        120 
TFVIAIPPPN ITGVLHMGHC LNTVLQDIVI RYQRMAGACT LWIPGTDHAG IATQHVVERA 

       130        140        150        160        170        180 
LRKEGIHKRE VTREQFVART QQIKDSHQDT IRMQLRKMGA SCDWTCERFT LDAGMSASVR 

       190        200        210        220        230        240 
EAFVTLYERG LLYRSMYLVN WCPRCGTALS DDEVFHQEKD GALYYVRYPL LPRTEEEGNG 

       250        260        270        280        290        300 
VPPPLGTAQV GETIIIATTR PETILADVAV AVHPDDARYQ SLIGRKVCVP MVNRIVPIIA 

       310        320        330        340        350        360 
DSYVAQDFGT GMVKITPAHD PNDWDIGTRH SLEAINMLNP DGSLNDQVPA AYRGLSCAQA 

       370        380        390        400        410        420 
RIQIVADLQA HGLLSREERI VHSVGVCYRC EAVIEPYLSL QWFVKMKPLA SQALAAWKRA 

       430        440        450        460        470        480 
DVQFHPKKWE NTYVRWLEHI RDWCISRQLW WGHRIPVWYC AQCAQQTVSR VDVQRCAHCG 

       490        500        510        520        530        540 
SADITQDPDV LDTWFSSWLW PFSTLGWPQE TQKLRAFYPT SAVITAYDII FFWVARMIMA 

       550        560        570        580        590        600 
GLEFTQTVPF RDVYLHGLVR DKQGRKMSKS LNNGVDPLHI IRTYGADALR FTLAFMCAQG 

       610        620        630        640        650        660 
QDVLIEMDSF KMGSRFANKV WNASRYILGN LEGRRVYAIA HVSLTELDRW IFHTFNETVQ 

       670        680        690        700        710        720 
QVRTALEAYR FNDAAQAVYE FFWNSFCDWY VEASKCSFQK PDEQEKDRAA SVLCTLLEET 

       730        740        750        760        770        780 
LRLLHPFLPF VTEEIYRSLS PSVHDTTQAI PSGAHALLMC APYPVYVPSR VDARACAHIG 

       790        800        810        820        830        840 
AVQEIVRAVR TLRAACGIDP QKAVSVRLRP SSPAQDANAA AQVSCVHDPG AVARTYEELI 

       850        860        870        880        890        900 
CVLAGISSLV YLESDAPKPQ VAVATAGTGF ELFLVTTEGI DRTMLCARLQ KAWQKARQKV 

       910        920        930        940        950 
QQVERKLADA QFCTHAPEEV VTAERKKLAE ARATCHTLAG YLADMNGKPG PLSDSD

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000520 Genomic DNA. Translation: AAC26589.1.
PIRB71250.
RefSeqNP_219472.1. NC_000919.1.

3D structure databases

ProteinModelPortalO83998.
ModBaseSearch...

Protein-protein interaction databases

STRING243276.TP1035.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC26589; AAC26589; TP_1035.
GeneID2610924.
KEGGtpa:TP1035.
PATRIC20532201. VBITrePal57110_1093.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0525.
KOK01873.
OMAIMDALIR.
ProtClustDBPRK05729.

Enzyme and pathway databases

BioCycTPAL243276:GC1H-1090-MONOMER.

Family and domain databases

Gene3D1.10.287.380. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 3 hits.
HAMAPMF_02004. Val_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERPTHR11946:SF5. PTHR11946:SF5. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSPR00986. TRNASYNTHVAL.
SUPFAMSSF46589. tRNA_binding_arm. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00422. valS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYV_TREPA
AccessionPrimary (citable) accession number: O83998
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1998
Last modified: May 29, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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