O83984 (GATB_TREPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-

Gene names

Name:	gatB
Ordered Locus Names:	TP_1021

Organism

Treponema pallidum (strain Nichols) [Complete proteome] [HAMAP]

Taxonomic identifier

243276 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Treponema

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP MF_00121
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP MF_00121 ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP MF_00121
Sequence similarities	Belongs to the GatB/GatE family. GatB subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-nitrogen ligase activity, with glutamine as amido-N-donor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP MF_00121		PRO_0000148861

Sequences

	Sequence		Length	Mass (Da)
	O83984 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: C28B90122236BF52	FASTA	509	56,865
	10 20 30 40 50 60 MSDLQTGTVP SIAGATDDTH AAPFFYEVII GCEIHCQLLT KTKAFCACAN RSGGMPNSRV 70 80 90 100 110 120 CPVCLGLPGA LPVVSEEYVR LGVRAGLALG CTIQLWSAFD RKHYFYPDLP KGYQITQYDA 130 140 150 160 170 180 PLCTDGAVDV QGVDMPVQRR VRIERIHLEE DAGKSLHAAD AYSYIDFNRC GVPLIEIVSR 190 200 210 220 230 240 PDLRSAEEAA CFMQTIREIL TFIEVTDGNL EEGALRCDAN VNVRILYKGQ EHHTPISEIK 250 260 270 280 290 300 NMNSYRMVRD ACTYEVQRQL QEFWQKGPAS KEEMQRKRTM GWDPVEGVTL LQRTKHSLRD 310 320 330 340 350 360 YRFMRDPDLP DLHLTPAYVQ HLSYTVGELP AARRARFKLD LGLSAFAAQT LTGSRMLADW 370 380 390 400 410 420 FEKAAHASKN ARRVANWILS EVLAVVNEKN ICIAELNLSP EAIAELMDAV EDQRITGKQA 430 440 450 460 470 480 KDIFAQMLAT GARAQDIISA QGLAQLSDEE EIATLVQTVF QEHPKALRDW QHGKTNVAAW 490 500 LMGQVMKRSR GRAHPARVAT LVHQALSQL

References

[1]

"Complete genome sequence of Treponema pallidum, the syphilis spirochete." Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G., Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A., Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D., Khalak H.G., Richardson D.L., Howell J.K. , Chidambaram M., Utterback T.R., McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A., Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O., Venter J.C. Science 281:375-388(1998) [PubMed: 9665876] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nichols.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000520 Genomic DNA. Translation: AAC65971.1.
PIR	A71254.
RefSeq	NP_219458.1. NC_000919.1.
3D structure databases
ProteinModelPortal	O83984.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2610877.
GenomeReviews	Gene locus TP_1021 in contig AE000520_GR.
KEGG	tpa:TP1021.
NMPDR	fig|243276.1.peg.1016.
PATRIC	20532169. VBITrePal57110_1077.
TIGR	TP_1021.
Phylogenomic databases
HOGENOM	HBG395951.
OMA	KNYFYAD.
ProtClustDB	PRK05477.
Enzyme and pathway databases
BioCyc	TPAL243276:TP_1021-MONOMER.
Family and domain databases
HAMAP	MF_00121. GatB. [Tree]
InterPro	IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR004413. Gln-tRNA_amidoTrfase_bsu. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
KO	K02434.
PANTHER	PTHR11659. GatB. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. GatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATB_TREPA

Accession

Primary (citable) accession number: O83984

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program