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O83856 (PNP_TREPA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Polyribonucleotide nucleotidyltransferase
EC=2.7.7.8
Alternative name(s):
Polynucleotide phosphorylase
Short name=PNPase
Gene names
Name:pnp
Ordered Locus Names:TP_0886
OrganismTreponema pallidum (strain Nichols) [Complete proteome] [HAMAP]
Taxonomic identifier243276 [NCBI]
Taxonomic lineageBacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Treponema

Protein attributes

Sequence length702 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity. HAMAP MF_01595

Catalytic activity

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595

Subunit structure

Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_01595.

Sequence similarities

Belongs to the polyribonucleotide nucleotidyltransferase family.

Contains 1 KH domain.

Contains 1 S1 motif domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandRNA-binding
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processRNA processing

Inferred from electronic annotation. Source: InterPro

mRNA catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function3'-5'-exoribonuclease activity

Inferred from electronic annotation. Source: InterPro

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

polyribonucleotide nucleotidyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 702702Polyribonucleotide nucleotidyltransferase HAMAP MF_01595
PRO_0000329921

Regions

Domain551 – 61060KH
Domain620 – 68869S1 motif

Sequences

Sequence LengthMass (Da)Tools
O83856 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: BBE97F7E822B2960

FASTA70276,332
        10         20         30         40         50         60 
MKHSITGTIG DHPLLLESGY LARQANGSVY LQCEGTAILA TVCSSAQRQE GLDYVPLTVD 

        70         80         90        100        110        120 
FNEKYYAVGK MPGGFIKREG RPKDREILIS RLIDRPMRPL FEKEFGRDIH VVPTCISSDM 

       130        140        150        160        170        180 
VHPHDVLAIV ASSAAVTLSD IPFHGPVAAV RVAYLNGSYV INPTFSQIDA ASMEVVVAGT 

       190        200        210        220        230        240 
RQGITMVEGG AREVSEDLML GALEQAQEHI KALCDMQERL RGLCGKEKQT VIPSSAQLVG 

       250        260        270        280        290        300 
RDRIYELAYP RLAQALYAQG KGERRSACDA VKRDVAQQYA AQLENDVQRR LFDALFHEME 

       310        320        330        340        350        360 
YEILRLNILD RGLRIDGRAI DAIRPIACEV GVLPRPHGSA VFTRGETQSL AVVTLGAMSD 

       370        380        390        400        410        420 
GQVYDDIEGD RRENFILHYN FPPFSVGEIG RMGVGRREIG HGCLAHRSLS AVIPDPEQFP 

       430        440        450        460        470        480 
YTVRVVSEIL ESNGSSSMAT VCSGTLSLLH AGVPIKKPVA GIAMGLITDG VRYAILSDIL 

       490        500        510        520        530        540 
GEEDHLGDMD FKVAGTCDGI TGFQMDVKVE AVSASLMKEA LQQARVGRLH ILSVMNQTIS 

       550        560        570        580        590        600 
APSVHISRYA PHIESFKIAV EKIGALIGPG GKTVKSLSDQ YRVTINTDSD GTVTVSGRDA 

       610        620        630        640        650        660 
QSVFDAKVAV VGLTEDPRVG RVYQGVVKRI VEFGAFVEIF PGKEGLCHVS KLSRSRVSKV 

       670        680        690        700 
SDVLQEGQRI CVKLIDIDRM GRLNLSYIDA LEGKSGGLDT TK

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000520 Genomic DNA. Translation: AAC26576.1.
PIRC71269.
RefSeqNP_219321.1. NC_000919.1.

3D structure databases

HSSPHSSP built from PDB template 1SRO based on UniProtKB P05055.
ProteinModelPortalO83856.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2611780.
GenomeReviewsGene locus TP_0886 in contig AE000520_GR.
KEGGtpa:TP0886.
NMPDRfig|243276.1.peg.880.
PATRIC20531881. VBITrePal57110_0938.
TIGRTP_0886.

Phylogenomic databases

HOGENOMHBG382411.
OMAYGETVVL.
ProtClustDBPRK11824.

Enzyme and pathway databases

BioCycTPAL243276:TP_0886-MONOMER.

Family and domain databases

HAMAPMF_01595. PNPase.
[Tree]
InterProIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH.
IPR004088. KH_type_1.
IPR018111. KH_type_1_subgr.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR012162. PNPase.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
KOK00962.
PANTHERPTHR11252. PNPase. 1 hit.
PfamPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFPIRSF005499. PNPase. 1 hit.
SMARTSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMSSF46915. 3_ExoRNase. 1 hit.
SSF55666. 3_ExoRNase. 2 hits.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF54211. Ribosomal_S5_D2-typ_fold. 2 hits.
TIGRFAMsTIGR03591. Polynuc_phos. 1 hit.
PROSITEPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePNP_TREPA
AccessionPrimary (citable) accession number: O83856
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: November 1, 1998
Last modified: January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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