Reviewed, UniProtKB/Swiss-Prot O83814 (AMPM_TREPA)

Names and origin

Protein names

Recommended name:
    Methionine aminopeptidase
      Short name=MAP
    EC=3.4.11.18
Alternative name(s):
    Peptidase M

Gene names

Name:	map
Ordered Locus Names:	TP_0842

Organism

Treponema pallidum [Complete proteome] [HAMAP]

Taxonomic identifier

160 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Treponema

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Methionine aminopeptidase		PRO_0000148966
Sites
	Metal binding	93	1	Cobalt 1 By similarity
	Metal binding	104	1	Cobalt 1 By similarity
	Metal binding	104	1	Cobalt 2 By similarity
	Metal binding	167	1	Cobalt 2 By similarity
	Metal binding	201	1	Cobalt 2 By similarity
	Metal binding	232	1	Cobalt 1 By similarity
	Metal binding	232	1	Cobalt 2 By similarity
	Binding site	76	1	Substrate By similarity
	Binding site	174	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O83814-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 4EDE582C67F3526A	FASTA	255	27,316
	10 20 30 40 50 60 MIRIKTPEQI DGIRASCKAL ARLFDVLIPL VKPGVQTQEL DAFCQRFIRS VGGVPAWFSE 70 80 90 100 110 120 GFPAAACISI NEEVIHGLPS ARVIQDGDLV SLDVGINLNG YISDACRTVP VGGVAHERLE 130 140 150 160 170 180 LLRVTTECLR AGIKACRAGA RVRAVSRAVY AVAARHRFGV VYEYCGHGVG LAVHEEPNIP 190 200 210 220 230 240 NVPGLEGPNP RFLPGMVVAI EPMLTLGTDE VRTSADGWTV VTADGSCACH VEHTVAVFAD 250 HTEVLTEPTE VERTG

References

[1]

"Complete genome sequence of Treponema pallidum, the syphilis spirochete." Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G., Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A., Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D., Khalak H.G., Richardson D.L., Howell J.K. , Chidambaram M., Utterback T.R., McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A., Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O., Venter J.C. Science 281:375-388(1998) [PubMed: 9665876] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nichols.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000520 Genomic DNA. Translation: AAC65810.1.
PIR	B71273.
RefSeq	NP_219278.1.
3D structure databases
SMR	O83814. Positions 2-252.
ModBase	Search...
Protein-protein interaction databases
IntAct	O83814. 7 interactions.
Genome annotation databases
GeneID	2611002.
GenomeReviews	Gene locus TP_0842 in contig AE000520_GR.
KEGG	tpa:TP0842.
NMPDR	fig|243276.1.peg.837.
TIGR	TP_0842.
Phylogenomic databases
HOGENOM	HBG299384.
OMA	REVETLD.
Enzyme and pathway databases
BioCyc	TPAL243276:TP_0842-MONOMER.
BRENDA	3.4.11.18. 142600.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHER	PTHR10804:SF13. Pept_M24A_MAP1. 1 hit. PTHR10804. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
TIGRFAMs	TIGR00500. met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_TREPA

Accession

Primary (citable) accession number: O83814

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1998
Last modified:	February 9, 2010
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)