Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O83774

- APBE_TREPA

UniProt

O83774 - APBE_TREPA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

FAD:protein FMN transferase

Gene

apbE

Organism
Treponema pallidum (strain Nichols)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Flavin transferase that catalyzes the transfer of the FMN moiety of FAD and its covalent binding to the hydroxyl group of a threonine residue in a target flavoprotein (By similarity). Displays FAD pyrophosphatase activity in vitro, hydrolyzing FAD into FMN and AMP (PubMed:23447540).By similarity1 Publication

Catalytic activityi

FAD + [protein]-L-threonine = [protein]-FMN-L-threonine + AMP.By similarity

Cofactori

Magnesium. Can also use manganese.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei181 – 1811FAD1 Publication
Metal bindingi184 – 1841Magnesium; via carbonyl oxygen1 Publication
Binding sitei187 – 1871FAD1 Publication
Metal bindingi306 – 3061Magnesium1 Publication
Metal bindingi310 – 3101Magnesium1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi118 – 1203FAD1 Publication
Nucleotide bindingi278 – 2803FAD1 Publication

GO - Biological processi

  1. protein flavinylation Source: InterPro
  2. thiamine biosynthetic process Source: UniProtKB-KW
  3. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

FAD, Flavoprotein, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciTPAL243276:GC1H-844-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
FAD:protein FMN transferaseBy similarity (EC:2.7.1.180By similarity)
Alternative name(s):
Flavin transferaseBy similarity
Gene namesi
Name:apbE
Ordered Locus Names:TP_0796
OrganismiTreponema pallidum (strain Nichols)
Taxonomic identifieri243276 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
ProteomesiUP000000811: Chromosome

Subcellular locationi

Cell inner membrane By similarityPROSITE-ProRule annotation; Lipid-anchor By similarityPROSITE-ProRule annotation; Periplasmic side By similarity

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222PROSITE-ProRule annotationAdd
BLAST
Chaini23 – 362340FAD:protein FMN transferasePRO_0000001751Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi23 – 231N-palmitoyl cysteinePROSITE-ProRule annotation
Lipidationi23 – 231S-diacylglycerol cysteinePROSITE-ProRule annotation

Keywords - PTMi

Lipoprotein, Palmitate

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi243276.TP0796.

Structurei

Secondary structure

1
362
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi30 – 389
Beta strandi41 – 499
Helixi53 – 7422
Beta strandi79 – 813
Helixi82 – 887
Turni89 – 913
Beta strandi95 – 973
Helixi99 – 11416
Turni115 – 1173
Helixi124 – 13512
Helixi142 – 1498
Helixi154 – 1563
Beta strandi157 – 1615
Beta strandi169 – 1735
Turni183 – 1853
Helixi186 – 20015
Beta strandi206 – 2105
Beta strandi213 – 2186
Beta strandi234 – 2385
Beta strandi245 – 2473
Beta strandi249 – 26315
Beta strandi266 – 2716
Beta strandi274 – 2785
Turni282 – 2843
Beta strandi285 – 2873
Beta strandi293 – 3008
Helixi302 – 31514
Helixi317 – 3248
Beta strandi331 – 3355
Beta strandi339 – 3435
Turni345 – 3473
Helixi348 – 3503
Beta strandi351 – 3533
Beta strandi358 – 3603

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IFUX-ray1.83A23-362[»]
4IFWX-ray2.30A23-362[»]
4IFXX-ray1.45A23-362[»]
4IFZX-ray1.90A23-362[»]
4IG1X-ray1.43A23-362[»]
ProteinModelPortaliO83774.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ApbE family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1477.
KOiK03734.
OMAiELMAINH.
OrthoDBiEOG6CGCF7.

Family and domain databases

InterProiIPR024932. ApbE.
IPR003374. ApbE-like.
[Graphical view]
PANTHERiPTHR30040:SF2. PTHR30040:SF2. 1 hit.
PfamiPF02424. ApbE. 1 hit.
[Graphical view]
PIRSFiPIRSF006268. ApbE. 1 hit.
SUPFAMiSSF143631. SSF143631. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O83774-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSSCVYWRI GVLVCILCGV GSCGGRARVR EYSRAELVIG TLCRVRVYSK
60 70 80 90 100
RPAAEVHAAL EEVFTLLQQQ EMVLSANRDD SALAALNAQA GSAPVVVDRS
110 120 130 140 150
LYALLERALF FAEKSGGAFN PALGAXVKLW NIGFDRAAVP DPDALKEALT
160 170 180 190 200
RCDFRQVHLR AGVSVGAPHT VQLAQAGMQL DLGAIAKGFL ADKIVQLLTA
210 220 230 240 250
HALDSALVDL GGNIFALGLK YGDVRSAAAQ RLEWNVGIRD PHGTGQKPAL
260 270 280 290 300
VVSVRDCSVV TSGAYERFFE RDGVRYHHII DPVTGFPAHT DVDSVSIFAP
310 320 330 340 350
RSTDADALAT ACFVLGYEKS CALLREFPGV DALFIFPDKR VRASAGIVDR
360
VRVLDARFVL ER
Length:362
Mass (Da):39,064
Last modified:November 1, 1998 - v1
Checksum:iE64E2FAFD1A7041C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000520 Genomic DNA. Translation: AAC65759.1.
PIRiC71281.
RefSeqiNP_219233.1. NC_000919.1.

Genome annotation databases

EnsemblBacteriaiAAC65759; AAC65759; TP_0796.
GeneIDi2610802.
KEGGitpa:TP0796.
PATRICi20531687. VBITrePal57110_0841.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000520 Genomic DNA. Translation: AAC65759.1 .
PIRi C71281.
RefSeqi NP_219233.1. NC_000919.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4IFU X-ray 1.83 A 23-362 [» ]
4IFW X-ray 2.30 A 23-362 [» ]
4IFX X-ray 1.45 A 23-362 [» ]
4IFZ X-ray 1.90 A 23-362 [» ]
4IG1 X-ray 1.43 A 23-362 [» ]
ProteinModelPortali O83774.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243276.TP0796.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC65759 ; AAC65759 ; TP_0796 .
GeneIDi 2610802.
KEGGi tpa:TP0796.
PATRICi 20531687. VBITrePal57110_0841.

Phylogenomic databases

eggNOGi COG1477.
KOi K03734.
OMAi ELMAINH.
OrthoDBi EOG6CGCF7.

Enzyme and pathway databases

BioCyci TPAL243276:GC1H-844-MONOMER.

Family and domain databases

InterProi IPR024932. ApbE.
IPR003374. ApbE-like.
[Graphical view ]
PANTHERi PTHR30040:SF2. PTHR30040:SF2. 1 hit.
Pfami PF02424. ApbE. 1 hit.
[Graphical view ]
PIRSFi PIRSF006268. ApbE. 1 hit.
SUPFAMi SSF143631. SSF143631. 1 hit.
PROSITEi PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nichols.
  2. "The TP0796 lipoprotein of Treponema pallidum is a bimetal-dependent FAD pyrophosphatase with a potential role in flavin homeostasis."
    Deka R.K., Brautigam C.A., Liu W.Z., Tomchick D.R., Norgard M.V.
    J. Biol. Chem. 288:11106-11121(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.43 ANGSTROMS) OF 23-362 IN COMPLEXES WITH ADP; AMP; FAD AND MAGNESIUM, FUNCTION, COFACTOR, SUBUNIT.

Entry informationi

Entry nameiAPBE_TREPA
AccessioniPrimary (citable) accession number: O83774
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3