O83687 (RLME_TREPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase E
EC=2.1.1.166

Alternative name(s):
23S rRNA Um2552 methyltransferase
rRNA (uridine-2'-O-)-methyltransferase

Gene names

Name:	rlmE
Synonyms:	ftsJ, rrmJ
Ordered Locus Names:	TP_0682

Organism

Treponema pallidum (strain Nichols) [Complete proteome] [HAMAP]

Taxonomic identifier

243276 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Treponema

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit By similarity. HAMAP MF_01547
Catalytic activity	S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2552) in 23S rRNA. HAMAP MF_01547
Subcellular location	Cytoplasm By similarity HAMAP MF_01547.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmE family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA methylation Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Ribosomal RNA large subunit methyltransferase E HAMAP MF_01547		PRO_0000155545
Sites
	Active site	151	1	Proton acceptor By similarity
	Binding site	51	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	53	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	71	1	S-adenosyl-L-methionine By similarity
	Binding site	90	1	S-adenosyl-L-methionine By similarity
	Binding site	112	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O83687 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: BE3F15687CD02B5A	FASTA	200	21,318
	10 20 30 40 50 60 MNVYKRADFW AKKAAAAGYR ARSVYKLAAL DKKYSLLSRA SRVLDLGAAP GSWTQYVLGT 70 80 90 100 110 120 AAACTAVCAV DVQPIASDIQ DARLQRVQGD LCAADTRARV ACNAPFDLIL SDAAPRTTGN 130 140 150 160 170 180 RTVDTSASAC LAAGVCAYVN FLSSDGGLVF KVFQGSEHLA ILTHLRAHFG AVCSFKPPAS 190 200 RPRSCELYVV ARFFRGTCGK

References

[1]

"Complete genome sequence of Treponema pallidum, the syphilis spirochete." Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G., Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A., Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D., Khalak H.G., Richardson D.L., Howell J.K. , Chidambaram M., Utterback T.R., McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A., Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O., Venter J.C. Science 281:375-388(1998) [PubMed: 9665876] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nichols.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000520 Genomic DNA. Translation: AAC65645.1.
PIR	B71295.
RefSeq	NP_219119.1. NC_000919.1.
3D structure databases
ProteinModelPortal	O83687.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2611272.
GenomeReviews	Gene locus TP_0682 in contig AE000520_GR.
KEGG	tpa:TP0682.
NMPDR	fig|243276.1.peg.678.
PATRIC	20531443. VBITrePal57110_0723.
TIGR	TP_0682.
Phylogenomic databases
HOGENOM	HBG398270.
OMA	WSQIAAK.
ProtClustDB	CLSK2300268.
Enzyme and pathway databases
BioCyc	TPAL243276:TP_0682-MONOMER.
Family and domain databases
HAMAP	MF_01547. 23SrRNA_methyltr_E. [Tree]
InterPro	IPR015507. rRNA-MeTfrase_E. IPR002877. rRNA_MeTrfase_RrmJ/FtsJ. [Graphical view]
KO	K02427.
PANTHER	PTHR10920. rRNA-MeTfrase_J. 1 hit.
Pfam	PF01728. FtsJ. 1 hit. [Graphical view]
PIRSF	PIRSF005461. 23S_rRNA_mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLME_TREPA

Accession

Primary (citable) accession number: O83687

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program