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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Treponema pallidum (strain Nichols)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi111IronUniRule annotation1
Metal bindingi115IronUniRule annotation1
Binding sitei166SubstrateUniRule annotation1
Binding sitei179Substrate; via amide nitrogenUniRule annotation1
Binding sitei275SubstrateUniRule annotation1
Metal bindingi300IronUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:TP_0680
OrganismiTreponema pallidum (strain Nichols)
Taxonomic identifieri243276 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
Proteomesi
  • UP000000811 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000969751 – 352tRNA N6-adenosine threonylcarbamoyltransferaseAdd BLAST352

Interactioni

Protein-protein interaction databases

STRINGi243276.TP0680.

Structurei

3D structure databases

ProteinModelPortaliO83686.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni133 – 137Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
KOiK01409.
OMAiICIDHIL.

Family and domain databases

HAMAPiMF_01445. TsaD. 1 hit.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O83686-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVLGIETSC DETAVAIVKD GTHVCSNVVA TQIPFHAPYR GIVPELASRK
60 70 80 90 100
HIEWILPTVK EALARAQLTL ADIDGIAVTH APGLTGSLLV GLTFAKTLAW
110 120 130 140 150
SMHLPFIAVN HLHAHFCAAH VEHDLAYPYV GLLASGGHAL VCVVHDFDQV
160 170 180 190 200
EALGATIDDA PGEAFDKVAA FYGFGYPGGK VIETLAEQGD ARAARFPLPH
210 220 230 240 250
FHGKGHRYDV SYSGLKTAVI HQLDHFWNKE YERTAQNIAA AFQACAINIL
260 270 280 290 300
LRPLARALQD TGLPTAVVCG GVAANSLLRK SVADWKHARC VFPSREYCTD
310 320 330 340 350
NAVMVAALGY RYLIRGDRSF YGVTERSRIA HFSKRGGDRL AAQRSAASQP

LF
Length:352
Mass (Da):38,132
Last modified:November 1, 1998 - v1
Checksum:i7597553A6AAD8C2F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000520 Genomic DNA. Translation: AAC65643.1.
PIRiH71294.
RefSeqiWP_010882125.1. NC_021490.2.

Genome annotation databases

EnsemblBacteriaiAAC65643; AAC65643; TP_0680.
GeneIDi2611265.
KEGGitpa:TP_0680.
PATRICi20531439. VBITrePal57110_0721.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000520 Genomic DNA. Translation: AAC65643.1.
PIRiH71294.
RefSeqiWP_010882125.1. NC_021490.2.

3D structure databases

ProteinModelPortaliO83686.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243276.TP0680.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC65643; AAC65643; TP_0680.
GeneIDi2611265.
KEGGitpa:TP_0680.
PATRICi20531439. VBITrePal57110_0721.

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
KOiK01409.
OMAiICIDHIL.

Family and domain databases

HAMAPiMF_01445. TsaD. 1 hit.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSAD_TREPA
AccessioniPrimary (citable) accession number: O83686
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.