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O83675 (DPO3A_TREPA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA polymerase III subunit alpha
EC=2.7.7.7
Gene names
Name:dnaE
Ordered Locus Names:TP_0669
OrganismTreponema pallidum (strain Nichols) [Complete proteome] [HAMAP]
Taxonomic identifier243276 [NCBI]
Taxonomic lineageBacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Treponema

Protein attributes

Sequence length1170 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase By similarity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Subunit structure

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the DNA polymerase type-C family. DnaE subfamily.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentCytoplasm
   Molecular functionDNA-directed DNA polymerase
Nucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function3'-5' exonuclease activity

Inferred from electronic annotation. Source: InterPro

DNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed DNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11701170DNA polymerase III subunit alpha
PRO_0000103356

Sequences

Sequence LengthMass (Da)Tools
O83675 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 431FC4629FDE09F1

FASTA1,170132,313
        10         20         30         40         50         60 
MARMSFVHLH VHSNYSLLDG ASSLQRLVRT AKSLGQEALA LTDHGNMFGA LHFQKVCSAE 

        70         80         90        100        110        120 
GIKAIIGCEL YVAPESRFDR SEHTIGRRYY HLIVLAKNET GYRNLMVLSS KAYIEGMYYK 

       130        140        150        160        170        180 
PRVDDELLAQ HAEGLICLSS CLAGQLPYLL LQGRKREAEE HARKYRALFG VDNYFIEVQD 

       190        200        210        220        230        240 
HGLDEEKKVA PLLIELACRL GIPLVVTNDV HYAEQEDSVA QDILLCIGTK KNRSDPNRLK 

       250        260        270        280        290        300 
FKTDEFYLKS SEKMAQLFPH YPEMVLNTVR IAQRCNVRIP QPGPLLPLYQ IPHEFSSKEH 

       310        320        330        340        350        360 
YIRHLVHRGL YDRYAVVSEE IKARADYELD VIVRMDFVGY FLIVWDFITW AKEHDIPVGP 

       370        380        390        400        410        420 
GRGSGASSIV AYALKITDID PLRYKLLFER FMNPERISMP DFDIDFCFER RQEVIEYVRA 

       430        440        450        460        470        480 
RYGNDNVGQI ITFGTLKPKA AIRDVGRVLD IPLSEVLMIT KLMPDDPKLT FKKAYESEQL 

       490        500        510        520        530        540 
AQMKQEPRYA ELFQIAEKLE DTNRNTSLHA AGIVIGKTAL TDYVPLYKDS KTGKISTQFG 

       550        560        570        580        590        600 
MDLIEDCGLV KMDFLGLKTL TLIQRTQNLV RRKGGKYTTF SISDISDQDP TTFSMLAEGK 

       610        620        630        640        650        660 
SAAVFQFESR GMQGILKRAK PSKMEDLIAL NALYRPGPMA FIDQYIESKR DPGKIKYPDP 

       670        680        690        700        710        720 
CLEDILSETY GVIVYQEQVM QVAQRIAGFS LGEADILRRA MGKKKLAVMQ EKKKEFAERA 

       730        740        750        760        770        780 
EKQGFDKKHA ENIFEILIPF AGYGFNKSHA TAYSVVAYQT AFLKANFPAE FMAANLSNEI 

       790        800        810        820        830        840 
NSAEKLPLYM AEAEKMGLSI QKPDVNASEP YFSVCEGCIV YGLLGIKGLG EQVAFDVFDE 

       850        860        870        880        890        900 
RIRNGPYTSF VEVLDRVPAT SLNKKNAEIM IKAGCFDRFG VTRASLTAHL DDAMKYVARK 

       910        920        930        940        950        960 
KAVTSSRQAS LFDETDLGEC SEYTFPVMEE WSQRERLRIE KELMGYYISG HPLDEYRSVI 

       970        980        990       1000       1010       1020 
GEKATLDLGH IENARSENKY LIVGVLNAIH PYTTKSGKNM AFGSFEDLHG SVDIVVFPVL 

      1030       1040       1050       1060       1070       1080 
WEEHRAQFLP ETIMGLVGTV DFSKETPAFL VDSVIDLEQL RFAQVKTILA GSEHRRVSSG 

      1090       1100       1110       1120       1130       1140 
EKTPLQKRGV SQEVHIEVSS HVRAHAQFKS LYEILSAHTG GSGEVFLHMH VDDRTYVVYV 

      1150       1160       1170 
PSCKVSATEV FAQQLKGNES FVQILKECVQ

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000520 Genomic DNA. Translation: AAC26567.1.
PIRH71295.
RefSeqNP_219106.1. NC_000919.1.

3D structure databases

ProteinModelPortalO83675.
ModBaseSearch...

Protein-protein interaction databases

IntActO83675. 2 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2611537.
GenomeReviewsGene locus TP_0669 in contig AE000520_GR.
KEGGtpa:TP0669.
NMPDRfig|243276.1.peg.665.
PATRIC20531413. VBITrePal57110_0710.
TIGRTP_0669.

Phylogenomic databases

HOGENOMHBG734490.
OMARVLQMPY.
ProtClustDBPRK05673.

Enzyme and pathway databases

BioCycTPAL243276:TP_0669-MONOMER.

Family and domain databases

InterProIPR011708. DNA_pol3_alpha.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
KOK02337.
PfamPF07733. DNA_pol3_alpha. 1 hit.
PF02811. PHP. 1 hit.
[Graphical view]
SMARTSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMSSF89550. PHP-like. 1 hit.
TIGRFAMsTIGR00594. Polc. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDPO3A_TREPA
AccessionPrimary (citable) accession number: O83675
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1998
Last modified: January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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