O83578 (ALKH_TREPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative KHG/KDPG aldolase

Including the following 2 domains:

1. 4-hydroxy-2-oxoglutarate aldolase
   EC=4.1.3.16
   Alternative name(s):
   2-keto-4-hydroxyglutarate aldolase
   Short name=KHG-aldolase
2. 2-dehydro-3-deoxy-phosphogluconate aldolase
   EC=4.1.2.14
   Alternative name(s):
   2-keto-3-deoxy-6-phosphogluconate aldolase
   Short name=KDPG-aldolase
   Phospho-2-dehydro-3-deoxygluconate aldolase
   Phospho-2-keto-3-deoxygluconate aldolase

Gene names

Name:	eda
Ordered Locus Names:	TP_0568

Organism

Treponema pallidum (strain Nichols) [Complete proteome] [HAMAP]

Taxonomic identifier

243276 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Spirochaetes › Spirochaetia › Spirochaetales › Spirochaetaceae › Treponema › Treponema pallidum › Treponema pallidum subsp. pallidum

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate. 2-dehydro-3-deoxy-D-gluconate 6-phosphate = pyruvate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate: step 2/2. Carbohydrate metabolism; glyoxylate and dicarboxylate metabolism.
Subunit structure	Homotrimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the KHG/KDPG aldolase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2-dehydro-3-deoxy-phosphogluconate aldolase activity Inferred from electronic annotation. Source: EC 4-hydroxy-2-oxoglutarate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Putative KHG/KDPG aldolase		PRO_0000201043
Sites
	Active site	41	1	By similarity
	Active site	45	1	By similarity
	Active site	129	1	Schiff-base intermediate with KHG or pyruvate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O83578 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 7DC3408B01C244E7	FASTA	210	22,074
	10 20 30 40 50 60 MITIFEALER VRVIPVVTLE RVEDAVPLAR ALITGGIRCM EVTFRTLVAA EAIAAIRQEC 70 80 90 100 110 120 ADVLLGAGTV LTVEQAQQAQ AAGAQFVVSP GFNPRVVAHC LGHGVPIIPG IASATEIERA 130 140 150 160 170 180 LEFGISVVKF FPAELLGGTA MMSALARPYT AVRFVPTGGI HLNNLAEYVA HPRVLACGGS 190 200 210 WMVPAQSIAA GDFSQVTALS QQTLQIVGVM

References

[1]

"Complete genome sequence of Treponema pallidum, the syphilis spirochete." Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G., Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A., Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D., Khalak H.G., Richardson D.L., Howell J.K. , Chidambaram M., Utterback T.R., McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A., Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O., Venter J.C. Science 281:375-388(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nichols.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000520 Genomic DNA. Translation: AAC65542.1.
PIR	F71308.
RefSeq	NP_219007.1. NC_000919.1.
3D structure databases
ProteinModelPortal	O83578.
ModBase	Search...
Protein-protein interaction databases
STRING	243276.TP0568.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC65542; AAC65542; TP_0568.
GeneID	2611476.
KEGG	tpa:TP0568.
PATRIC	20531189. VBITrePal57110_0607.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0800.
KO	K01625.
OMA	AQFAVSP.
ProtClustDB	CLSK2300246.
Enzyme and pathway databases
UniPathway	UPA00227. UPA00856; UER00829.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR000887. Aldlse_KDPG_KHG. IPR013785. Aldolase_TIM. [Graphical view]
Pfam	PF01081. Aldolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01182. eda. 1 hit.
PROSITE	PS00159. ALDOLASE_KDPG_KHG_1. 1 hit. PS00160. ALDOLASE_KDPG_KHG_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALKH_TREPA

Accession

Primary (citable) accession number: O83578

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program