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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Treponema pallidum (strain Nichols)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86S-adenosyl-L-methionineUniRule annotation1
Binding sitei111S-adenosyl-L-methionineUniRule annotation1
Binding sitei138S-adenosyl-L-methionineUniRule annotation1
Active sitei161By similarity1
Binding sitei161S-adenosyl-L-methionineUniRule annotation1
Binding sitei165SubstrateUniRule annotation1
Binding sitei197SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:TP_0464
OrganismiTreponema pallidum (strain Nichols)
Taxonomic identifieri243276 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
Proteomesi
  • UP000000811 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001714161 – 250tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST250

Interactioni

Protein-protein interaction databases

IntActiO83477. 4 interactors.
MINTiMINT-6479630.
STRINGi243276.TP0464.

Structurei

3D structure databases

ProteinModelPortaliO83477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni229 – 232Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107W7Y. Bacteria.
COG0220. LUCA.
KOiK03439.
OMAiIKLGHGV.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O83477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNDSARMRK VLTFTRRSNR MTACQKRDYQ HLASRWIIPY QNTVFDYAAV
60 70 80 90 100
FCSPAAPSAP AGAFPAPQGK TDAVAPACAP APLVVEIGFG MGSATAAIAA
110 120 130 140 150
RNPHLSYLGI EVYRAGIGRL LRKIEAERLH NLRIIEHDAL DVLRTMIAPQ
160 170 180 190 200
TLAGLHIFFP DPWPKTRHHK RRLLYRPRTD LLARALAPGG YLYAVTDWAE
210 220 230 240 250
YARRAQEELA RTPSLTWAPQ GARPWRPATE FERKAQTQGR AIHELFFIKA
Length:250
Mass (Da):28,068
Last modified:November 1, 1998 - v1
Checksum:iC5A227F4E4D15CC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000520 Genomic DNA. Translation: AAC65448.1.
PIRiB71321.
RefSeqiWP_010881913.1. NC_021490.2.

Genome annotation databases

EnsemblBacteriaiAAC65448; AAC65448; TP_0464.
GeneIDi2611075.
KEGGitpa:TP_0464.
PATRICi20530953. VBITrePal57110_0496.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000520 Genomic DNA. Translation: AAC65448.1.
PIRiB71321.
RefSeqiWP_010881913.1. NC_021490.2.

3D structure databases

ProteinModelPortaliO83477.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO83477. 4 interactors.
MINTiMINT-6479630.
STRINGi243276.TP0464.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC65448; AAC65448; TP_0464.
GeneIDi2611075.
KEGGitpa:TP_0464.
PATRICi20530953. VBITrePal57110_0496.

Phylogenomic databases

eggNOGiENOG4107W7Y. Bacteria.
COG0220. LUCA.
KOiK03439.
OMAiIKLGHGV.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_TREPA
AccessioniPrimary (citable) accession number: O83477
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.