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O83477 (TRMB_TREPA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:TP_0464
OrganismTreponema pallidum (strain Nichols) [Complete proteome] [HAMAP]
Taxonomic identifier243276 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 250250tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171416

Regions

Region229 – 2324Substrate binding By similarity

Sites

Active site1611 By similarity
Binding site861S-adenosyl-L-methionine By similarity
Binding site1111S-adenosyl-L-methionine By similarity
Binding site1381S-adenosyl-L-methionine By similarity
Binding site1611S-adenosyl-L-methionine By similarity
Binding site1651Substrate By similarity
Binding site1971Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O83477 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: C5A227F4E4D15CC0

FASTA25028,068
        10         20         30         40         50         60 
MTNDSARMRK VLTFTRRSNR MTACQKRDYQ HLASRWIIPY QNTVFDYAAV FCSPAAPSAP 

        70         80         90        100        110        120 
AGAFPAPQGK TDAVAPACAP APLVVEIGFG MGSATAAIAA RNPHLSYLGI EVYRAGIGRL 

       130        140        150        160        170        180 
LRKIEAERLH NLRIIEHDAL DVLRTMIAPQ TLAGLHIFFP DPWPKTRHHK RRLLYRPRTD 

       190        200        210        220        230        240 
LLARALAPGG YLYAVTDWAE YARRAQEELA RTPSLTWAPQ GARPWRPATE FERKAQTQGR 

       250 
AIHELFFIKA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000520 Genomic DNA. Translation: AAC65448.1.
PIRB71321.
RefSeqNP_218905.1. NC_000919.1.
YP_008091312.1. NC_021490.2.

3D structure databases

ProteinModelPortalO83477.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO83477. 4 interactions.
MINTMINT-6479630.
STRING243276.TP0464.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC65448; AAC65448; TP_0464.
GeneID15851752.
2611075.
KEGGtpa:TP0464.
tpw:TPANIC_0464.
PATRIC20530953. VBITrePal57110_0496.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMAMSTENEN.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycTPAL243276:GC1H-491-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_TREPA
AccessionPrimary (citable) accession number: O83477
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways