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O83466

- SYI_TREPA

UniProt

O83466 - SYI_TREPA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Treponema pallidum (strain Nichols)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei628 – 6281ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTPAL243276:GC1H-479-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:TP_0452
OrganismiTreponema pallidum (strain Nichols)
Taxonomic identifieri243276 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
ProteomesiUP000000811: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10911091Isoleucine--tRNA ligasePRO_0000098569Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

IntActiO83466. 3 interactions.
STRINGi243276.TP0452.

Structurei

3D structure databases

ProteinModelPortaliO83466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi625 – 6295"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

O83466-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYTPVDPKVD FVAQERRILA FWRERRVFEQ SVAQRAQGKS YVFFDGPPFA
60 70 80 90 100
TGLPHFGHFV PSTIKDIIPR YQTMRGAYVP RRFGWDCHGL PIEHLIEQEL
110 120 130 140 150
NLNSKSDVES YGVSAFNAAC RSSVLRYVKE WQRTLTRLGR WVDFDNDYKT
160 170 180 190 200
MDVCYMESVW WVVAQLWQRK LLYEGYKILP YCPRCATALS NHELNLGGYQ
210 220 230 240 250
DVSDPAITVR FECTSVVPGS PAAREFCAAA SWGSASLPAH TCFLAWTTTP
260 270 280 290 300
WTLPCNAALA LGPQILYVLI EANDEHYILA RSRLEFYYPD SSAYRVVWEK
310 320 330 340 350
RGEHLAGIRY RPLFSYPVFG QGPDPSVQGD SEEGLFCTRV ADFVSTEDGT
360 370 380 390 400
GVVHVAPAFG EDDYEVFKDA GISIQCPLDA ECRFTAEVAD YQGLFVKAAD
410 420 430 440 450
KAIIARVQKQ GALFRREQIS HAYPHCWRCA SPLIYRAVHS WFVAVEKIKD
460 470 480 490 500
KMLAANASIC WQPSHIRDGR FGKWLVCARD WAISRDRYWG NPLPIWRCVH
510 520 530 540 550
CGATDCIGSR TQLYERSGML LEDLHKHVVD MVTIPCACGS VMRRVPEVLD
560 570 580 590 600
CWFESGAMPY AQQHYPFEHA TDFERYFPAH FISEGLDQTR GWFYTLTILA
610 620 630 640 650
VALFERPAFE NCIVTGLVLA SDGKKMSKAL RNYADPNEVM DRYGADALRL
660 670 680 690 700
FLVRSAVVRA DDLKYSDEGV KDILKTVIIP LWNSYSFYVT YANIDGIDPP
710 720 730 740 750
VCAKVDGMGQ AVTRLATHLN NPLDRWILSL TEKLVQDIAC ALDAYDVSKV
760 770 780 790 800
ADPIVSYVDQ LNNWYIRRSR RRFWKSINDE DKRCAYNTLY CVLKRCVLAI
810 820 830 840 850
APVVPFITES IWQNIRAADD VQSVHLADYP VCTPMVRDDA LEFKMETVQR
860 870 880 890 900
VVSMARAIRA QCNLKVRQPL KAMQVITRNP MERSALLEME EDVLDELNVK
910 920 930 940 950
ELVFHEKEDE IVEYRAKANF RVLGKELGSK TKRAALSIER LSSAEIQEIL
960 970 980 990 1000
EGTTLYLDVD GDRLELTEEK ILVQRIERES LKAINEGTLT VALDTTLTED
1010 1020 1030 1040 1050
LLLEGAIRDL VRGVQNLRKE RGFSLVDRIC LRVFSSDQDI VCARKAYDLH
1060 1070 1080 1090
RSYIVGETLA AHVQWARVRD GASAVYVKSD AVLWEVSIDK A
Length:1,091
Mass (Da):124,242
Last modified:November 1, 1998 - v1
Checksum:i30FCAE18C6F301A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000520 Genomic DNA. Translation: AAC65439.1.
PIRiE71322.
RefSeqiNP_218893.1. NC_000919.1.
YP_008091301.1. NC_021490.2.

Genome annotation databases

EnsemblBacteriaiAAC65439; AAC65439; TP_0452.
GeneIDi15851741.
2611024.
KEGGitpa:TP0452.
tpw:TPANIC_0452.
PATRICi20530923. VBITrePal57110_0481.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000520 Genomic DNA. Translation: AAC65439.1 .
PIRi E71322.
RefSeqi NP_218893.1. NC_000919.1.
YP_008091301.1. NC_021490.2.

3D structure databases

ProteinModelPortali O83466.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O83466. 3 interactions.
STRINGi 243276.TP0452.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC65439 ; AAC65439 ; TP_0452 .
GeneIDi 15851741.
2611024.
KEGGi tpa:TP0452.
tpw:TPANIC_0452.
PATRICi 20530923. VBITrePal57110_0481.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TPAL243276:GC1H-479-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nichols.

Entry informationi

Entry nameiSYI_TREPA
AccessioniPrimary (citable) accession number: O83466
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1998
Last modified: November 26, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3