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O83466 (SYI_TREPA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:TP_0452
OrganismTreponema pallidum (strain Nichols) [Complete proteome] [HAMAP]
Taxonomic identifier243276 [NCBI]
Taxonomic lineageBacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Treponema ›

Protein attributes

Sequence length1091 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003

Cofactor

Zinc By similarity.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10911091Isoleucine--tRNA ligase HAMAP-Rule MF_02003
PRO_0000098569

Regions

Motif48 – 5811"HIGH" region HAMAP-Rule MF_02003
Motif625 – 6295"KMSKS" region HAMAP-Rule MF_02003

Sites

Binding site6281ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
O83466 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 30FCAE18C6F301A2

FASTA1,091124,242
        10         20         30         40         50         60 
MYTPVDPKVD FVAQERRILA FWRERRVFEQ SVAQRAQGKS YVFFDGPPFA TGLPHFGHFV 

        70         80         90        100        110        120 
PSTIKDIIPR YQTMRGAYVP RRFGWDCHGL PIEHLIEQEL NLNSKSDVES YGVSAFNAAC 

       130        140        150        160        170        180 
RSSVLRYVKE WQRTLTRLGR WVDFDNDYKT MDVCYMESVW WVVAQLWQRK LLYEGYKILP 

       190        200        210        220        230        240 
YCPRCATALS NHELNLGGYQ DVSDPAITVR FECTSVVPGS PAAREFCAAA SWGSASLPAH 

       250        260        270        280        290        300 
TCFLAWTTTP WTLPCNAALA LGPQILYVLI EANDEHYILA RSRLEFYYPD SSAYRVVWEK 

       310        320        330        340        350        360 
RGEHLAGIRY RPLFSYPVFG QGPDPSVQGD SEEGLFCTRV ADFVSTEDGT GVVHVAPAFG 

       370        380        390        400        410        420 
EDDYEVFKDA GISIQCPLDA ECRFTAEVAD YQGLFVKAAD KAIIARVQKQ GALFRREQIS 

       430        440        450        460        470        480 
HAYPHCWRCA SPLIYRAVHS WFVAVEKIKD KMLAANASIC WQPSHIRDGR FGKWLVCARD 

       490        500        510        520        530        540 
WAISRDRYWG NPLPIWRCVH CGATDCIGSR TQLYERSGML LEDLHKHVVD MVTIPCACGS 

       550        560        570        580        590        600 
VMRRVPEVLD CWFESGAMPY AQQHYPFEHA TDFERYFPAH FISEGLDQTR GWFYTLTILA 

       610        620        630        640        650        660 
VALFERPAFE NCIVTGLVLA SDGKKMSKAL RNYADPNEVM DRYGADALRL FLVRSAVVRA 

       670        680        690        700        710        720 
DDLKYSDEGV KDILKTVIIP LWNSYSFYVT YANIDGIDPP VCAKVDGMGQ AVTRLATHLN 

       730        740        750        760        770        780 
NPLDRWILSL TEKLVQDIAC ALDAYDVSKV ADPIVSYVDQ LNNWYIRRSR RRFWKSINDE 

       790        800        810        820        830        840 
DKRCAYNTLY CVLKRCVLAI APVVPFITES IWQNIRAADD VQSVHLADYP VCTPMVRDDA 

       850        860        870        880        890        900 
LEFKMETVQR VVSMARAIRA QCNLKVRQPL KAMQVITRNP MERSALLEME EDVLDELNVK 

       910        920        930        940        950        960 
ELVFHEKEDE IVEYRAKANF RVLGKELGSK TKRAALSIER LSSAEIQEIL EGTTLYLDVD 

       970        980        990       1000       1010       1020 
GDRLELTEEK ILVQRIERES LKAINEGTLT VALDTTLTED LLLEGAIRDL VRGVQNLRKE 

      1030       1040       1050       1060       1070       1080 
RGFSLVDRIC LRVFSSDQDI VCARKAYDLH RSYIVGETLA AHVQWARVRD GASAVYVKSD 

      1090 
AVLWEVSIDK A

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000520 Genomic DNA. Translation: AAC65439.1.
PIRE71322.
RefSeqNP_218893.1. NC_000919.1.

3D structure databases

ProteinModelPortalO83466.
ModBaseSearch...

Protein-protein interaction databases

IntActO83466. 3 interactions.
STRING243276.TP0452.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC65439; AAC65439; TP_0452.
GeneID2611024.
KEGGtpa:TP0452.
PATRIC20530923. VBITrePal57110_0481.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
KOK01870.
OMASRSRYWG.
ProtClustDBPRK06039.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02003. Ile_tRNA_synth_type2.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_TREPA
AccessionPrimary (citable) accession number: O83466
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1998
Last modified: May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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