Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O83466

- SYI_TREPA

UniProt

O83466 - SYI_TREPA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Treponema pallidum (strain Nichols)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei628 – 6281ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciTPAL243276:GC1H-479-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:TP_0452
    OrganismiTreponema pallidum (strain Nichols)
    Taxonomic identifieri243276 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
    ProteomesiUP000000811: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10911091Isoleucine--tRNA ligasePRO_0000098569Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    IntActiO83466. 3 interactions.
    STRINGi243276.TP0452.

    Structurei

    3D structure databases

    ProteinModelPortaliO83466.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi625 – 6295"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O83466-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYTPVDPKVD FVAQERRILA FWRERRVFEQ SVAQRAQGKS YVFFDGPPFA     50
    TGLPHFGHFV PSTIKDIIPR YQTMRGAYVP RRFGWDCHGL PIEHLIEQEL 100
    NLNSKSDVES YGVSAFNAAC RSSVLRYVKE WQRTLTRLGR WVDFDNDYKT 150
    MDVCYMESVW WVVAQLWQRK LLYEGYKILP YCPRCATALS NHELNLGGYQ 200
    DVSDPAITVR FECTSVVPGS PAAREFCAAA SWGSASLPAH TCFLAWTTTP 250
    WTLPCNAALA LGPQILYVLI EANDEHYILA RSRLEFYYPD SSAYRVVWEK 300
    RGEHLAGIRY RPLFSYPVFG QGPDPSVQGD SEEGLFCTRV ADFVSTEDGT 350
    GVVHVAPAFG EDDYEVFKDA GISIQCPLDA ECRFTAEVAD YQGLFVKAAD 400
    KAIIARVQKQ GALFRREQIS HAYPHCWRCA SPLIYRAVHS WFVAVEKIKD 450
    KMLAANASIC WQPSHIRDGR FGKWLVCARD WAISRDRYWG NPLPIWRCVH 500
    CGATDCIGSR TQLYERSGML LEDLHKHVVD MVTIPCACGS VMRRVPEVLD 550
    CWFESGAMPY AQQHYPFEHA TDFERYFPAH FISEGLDQTR GWFYTLTILA 600
    VALFERPAFE NCIVTGLVLA SDGKKMSKAL RNYADPNEVM DRYGADALRL 650
    FLVRSAVVRA DDLKYSDEGV KDILKTVIIP LWNSYSFYVT YANIDGIDPP 700
    VCAKVDGMGQ AVTRLATHLN NPLDRWILSL TEKLVQDIAC ALDAYDVSKV 750
    ADPIVSYVDQ LNNWYIRRSR RRFWKSINDE DKRCAYNTLY CVLKRCVLAI 800
    APVVPFITES IWQNIRAADD VQSVHLADYP VCTPMVRDDA LEFKMETVQR 850
    VVSMARAIRA QCNLKVRQPL KAMQVITRNP MERSALLEME EDVLDELNVK 900
    ELVFHEKEDE IVEYRAKANF RVLGKELGSK TKRAALSIER LSSAEIQEIL 950
    EGTTLYLDVD GDRLELTEEK ILVQRIERES LKAINEGTLT VALDTTLTED 1000
    LLLEGAIRDL VRGVQNLRKE RGFSLVDRIC LRVFSSDQDI VCARKAYDLH 1050
    RSYIVGETLA AHVQWARVRD GASAVYVKSD AVLWEVSIDK A 1091
    Length:1,091
    Mass (Da):124,242
    Last modified:November 1, 1998 - v1
    Checksum:i30FCAE18C6F301A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000520 Genomic DNA. Translation: AAC65439.1.
    PIRiE71322.
    RefSeqiNP_218893.1. NC_000919.1.
    YP_008091301.1. NC_021490.2.

    Genome annotation databases

    EnsemblBacteriaiAAC65439; AAC65439; TP_0452.
    GeneIDi15851741.
    2611024.
    KEGGitpa:TP0452.
    tpw:TPANIC_0452.
    PATRICi20530923. VBITrePal57110_0481.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000520 Genomic DNA. Translation: AAC65439.1 .
    PIRi E71322.
    RefSeqi NP_218893.1. NC_000919.1.
    YP_008091301.1. NC_021490.2.

    3D structure databases

    ProteinModelPortali O83466.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O83466. 3 interactions.
    STRINGi 243276.TP0452.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC65439 ; AAC65439 ; TP_0452 .
    GeneIDi 15851741.
    2611024.
    KEGGi tpa:TP0452.
    tpw:TPANIC_0452.
    PATRICi 20530923. VBITrePal57110_0481.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci TPAL243276:GC1H-479-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Nichols.

    Entry informationi

    Entry nameiSYI_TREPA
    AccessioniPrimary (citable) accession number: O83466
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3