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O83409 (TOP1_TREPA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:TP_0394
OrganismTreponema pallidum (strain Nichols) [Complete proteome] [HAMAP]
Taxonomic identifier243276 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema

Protein attributes

Sequence length731 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 731731DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145170

Regions

Domain17 – 130114Toprim
Zinc finger591 – 61727C4-type 1 HAMAP-Rule MF_00952
Zinc finger628 – 65730C4-type 2 HAMAP-Rule MF_00952
Zinc finger670 – 69627C4-type 3 HAMAP-Rule MF_00952
Region178 – 1836Interaction with DNA By similarity

Sites

Active site3121O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding231Magnesium 1; catalytic By similarity
Metal binding961Magnesium 1; catalytic By similarity
Metal binding961Magnesium 2 By similarity
Metal binding981Magnesium 2 By similarity
Site471Interaction with DNA By similarity
Site1541Interaction with DNA By similarity
Site1551Interaction with DNA By similarity
Site1581Interaction with DNA By similarity
Site1631Interaction with DNA By similarity
Site1701Interaction with DNA By similarity
Site3141Interaction with DNA By similarity
Site5011Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
O83409 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: C0F0C6C3DDD16F29

FASTA73182,441
        10         20         30         40         50         60 
MEVRGLQPKR QKTFARKHLV IVESPAKAQT IEKYLGTQYV VRASMGHVID LPKSRLAIDI 

        70         80         90        100        110        120 
EHDFQPEYIT VRGRAQCLKE LRTLSKQSLQ VFLASDRDRE GEAIAYHLAQ SIQAYCDTPI 

       130        140        150        160        170        180 
KRIVFNEITP HAIRAAIGHP VPIDTAKVNA QKARRVLDRL VGYHLCPLLW HKVKNGLSAG 

       190        200        210        220        230        240 
RVQSVALRLI CEREVEVKRF VPEEYWTVEG TFEKDKKSFS ALLILIQGKK AVFKSKQEAT 

       250        260        270        280        290        300 
SAIGLFSQSE ARVSQIRSFE KNVRPKQPFT TSTLQQCAAN RLGFTSRKTM QVAQQLYEGV 

       310        320        330        340        350        360 
SLGTHRVGLI TYMRTDSVRV SEAAVKEVRA WIATHFSDAL PGTPNRYAAK GKSQDAHEAI 

       370        380        390        400        410        420 
RPTYVAHTPE RIKAHLTRDQ IRLYTLIWER FVASQMTDAR VRSLTFEITA GPAVFSATET 

       430        440        450        460        470        480 
QVIEQGFYRV LKMLSPKDLS KAVLPPTKEG EVVALHNVQS VQHFTQGPVR YTDASIVKML 

       490        500        510        520        530        540 
EEKGIGRPST YAPTISVLLD RYYVTRIQKQ LMPTPLGKVI SDLLTTYFHD VVDVSFTARM 

       550        560        570        580        590        600 
ESKLDEVEED KIKWNCVIAD FYPAFSEKVS TVMKDLNSMR GVFDEKTDVV CSQCGDTMVK 

       610        620        630        640        650        660 
KLGRFGFFLA CGKFPECRNT QPVPLAKCPR PACDGNIVGK KTRGRKEFYG CTRFPVCDFV 

       670        680        690        700        710        720 
THFKPTFAVC PQCRCFLVEK SNRRVGTYTA CVNPECRYVS PTRKNLSLGK EAVLDGSHRG 

       730 
AQDKGAVQHY E 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000520 Genomic DNA. Translation: AAC65378.1.
PIRD71332.
RefSeqNP_218834.1. NC_000919.1.
YP_008091243.1. NC_021490.2.

3D structure databases

ProteinModelPortalO83409.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO83409. 2 interactions.
STRING243276.TP0394.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC65378; AAC65378; TP_0394.
GeneID15851683.
2610767.
KEGGtpa:TP0394.
tpw:TPANIC_0394.
PATRIC20530797. VBITrePal57110_0418.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0551.
KOK03168.
OMAMVYKMGR.
OrthoDBEOG6S7XQ9.
ProtClustDBPRK05582.

Enzyme and pathway databases

BioCycTPAL243276:GC1H-420-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_TREPA
AccessionPrimary (citable) accession number: O83409
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1998
Last modified: February 19, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families