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O83409

- TOP1_TREPA

UniProt

O83409 - TOP1_TREPA

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Protein
DNA topoisomerase 1
Gene
topA, TP_0394
Organism
Treponema pallidum (strain Nichols)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi23 – 231Magnesium 1; catalytic By similarity
Sitei47 – 471Interaction with DNA By similarity
Metal bindingi96 – 961Magnesium 1; catalytic By similarity
Metal bindingi96 – 961Magnesium 2 By similarity
Metal bindingi98 – 981Magnesium 2 By similarity
Sitei154 – 1541Interaction with DNA By similarity
Sitei155 – 1551Interaction with DNA By similarity
Sitei158 – 1581Interaction with DNA By similarity
Sitei163 – 1631Interaction with DNA By similarity
Sitei170 – 1701Interaction with DNA By similarity
Active sitei312 – 3121O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei314 – 3141Interaction with DNA By similarity
Sitei501 – 5011Interaction with DNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri591 – 61727C4-type 1UniRule annotation
Add
BLAST
Zinc fingeri628 – 65730C4-type 2UniRule annotation
Add
BLAST
Zinc fingeri670 – 69627C4-type 3UniRule annotation
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciTPAL243276:GC1H-420-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:TP_0394
OrganismiTreponema pallidum (strain Nichols)
Taxonomic identifieri243276 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
ProteomesiUP000000811: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 731731DNA topoisomerase 1UniRule annotation
PRO_0000145170Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

IntActiO83409. 2 interactions.
STRINGi243276.TP0394.

Structurei

3D structure databases

ProteinModelPortaliO83409.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 130114Toprim
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni178 – 1836Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
KOiK03168.
OMAiKEYNNWQ.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O83409-1 [UniParc]FASTAAdd to Basket

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MEVRGLQPKR QKTFARKHLV IVESPAKAQT IEKYLGTQYV VRASMGHVID    50
LPKSRLAIDI EHDFQPEYIT VRGRAQCLKE LRTLSKQSLQ VFLASDRDRE 100
GEAIAYHLAQ SIQAYCDTPI KRIVFNEITP HAIRAAIGHP VPIDTAKVNA 150
QKARRVLDRL VGYHLCPLLW HKVKNGLSAG RVQSVALRLI CEREVEVKRF 200
VPEEYWTVEG TFEKDKKSFS ALLILIQGKK AVFKSKQEAT SAIGLFSQSE 250
ARVSQIRSFE KNVRPKQPFT TSTLQQCAAN RLGFTSRKTM QVAQQLYEGV 300
SLGTHRVGLI TYMRTDSVRV SEAAVKEVRA WIATHFSDAL PGTPNRYAAK 350
GKSQDAHEAI RPTYVAHTPE RIKAHLTRDQ IRLYTLIWER FVASQMTDAR 400
VRSLTFEITA GPAVFSATET QVIEQGFYRV LKMLSPKDLS KAVLPPTKEG 450
EVVALHNVQS VQHFTQGPVR YTDASIVKML EEKGIGRPST YAPTISVLLD 500
RYYVTRIQKQ LMPTPLGKVI SDLLTTYFHD VVDVSFTARM ESKLDEVEED 550
KIKWNCVIAD FYPAFSEKVS TVMKDLNSMR GVFDEKTDVV CSQCGDTMVK 600
KLGRFGFFLA CGKFPECRNT QPVPLAKCPR PACDGNIVGK KTRGRKEFYG 650
CTRFPVCDFV THFKPTFAVC PQCRCFLVEK SNRRVGTYTA CVNPECRYVS 700
PTRKNLSLGK EAVLDGSHRG AQDKGAVQHY E 731
Length:731
Mass (Da):82,441
Last modified:November 1, 1998 - v1
Checksum:iC0F0C6C3DDD16F29
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000520 Genomic DNA. Translation: AAC65378.1.
PIRiD71332.
RefSeqiNP_218834.1. NC_000919.1.
YP_008091243.1. NC_021490.2.

Genome annotation databases

EnsemblBacteriaiAAC65378; AAC65378; TP_0394.
GeneIDi15851683.
2610767.
KEGGitpa:TP0394.
tpw:TPANIC_0394.
PATRICi20530797. VBITrePal57110_0418.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000520 Genomic DNA. Translation: AAC65378.1 .
PIRi D71332.
RefSeqi NP_218834.1. NC_000919.1.
YP_008091243.1. NC_021490.2.

3D structure databases

ProteinModelPortali O83409.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O83409. 2 interactions.
STRINGi 243276.TP0394.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC65378 ; AAC65378 ; TP_0394 .
GeneIDi 15851683.
2610767.
KEGGi tpa:TP0394.
tpw:TPANIC_0394.
PATRICi 20530797. VBITrePal57110_0418.

Phylogenomic databases

eggNOGi COG0551.
KOi K03168.
OMAi KEYNNWQ.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci TPAL243276:GC1H-420-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nichols.

Entry informationi

Entry nameiTOP1_TREPA
AccessioniPrimary (citable) accession number: O83409
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1998
Last modified: September 3, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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