ID   MURF_TREPA              Reviewed;         459 AA.
AC   O83401;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   16-JUN-2009, entry version 65.
DE   RecName: Full=UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase;
DE            EC=6.3.2.10;
DE   AltName: Full=UDP-MurNAc-pentapeptide synthetase;
DE   AltName: Full=D-alanyl-D-alanine-adding enzyme;
GN   Name=murF; OrderedLocusNames=TP_0386;
OS   Treponema pallidum.
OC   Bacteria; Spirochaetes; Spirochaetales; Spirochaetaceae; Treponema.
OX   NCBI_TaxID=160;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Nichols;
RX   MEDLINE=98332770; PubMed=9665876; DOI=10.1126/science.281.5375.375;
RA   Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G.,
RA   Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A.,
RA   Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D.,
RA   Khalak H.G., Richardson D.L., Howell J.K., Chidambaram M.,
RA   Utterback T.R., McDonald L.A., Artiach P., Bowman C., Cotton M.D.,
RA   Fujii C., Garland S.A., Hatch B., Horst K., Roberts K.M., Sandusky M.,
RA   Weidman J.F., Smith H.O., Venter J.C.;
RT   "Complete genome sequence of Treponema pallidum, the syphilis
RT   spirochete.";
RL   Science 281:375-388(1998).
CC   -!- FUNCTION: Involved in cell wall formation. Catalyzes the final
CC       step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the
CC       precursor of murein (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-
CC       glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-
CC       acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-
CC       alanine.
CC   -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the murCDEF family.
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DR   EMBL; AE000520; AAC65370.1; -; Genomic_DNA.
DR   PIR; D71331; D71331.
DR   RefSeq; NP_218826.1; -.
DR   HSSP; P11880; 1GG4.
DR   IntAct; O83401; 1.
DR   GeneID; 2611288; -.
DR   GenomeReviews; AE000520_GR; TP_0386.
DR   KEGG; tpa:TP0386; -.
DR   NMPDR; fig|243276.1.peg.385; -.
DR   TIGR; TP_0386; -.
DR   HOGENOM; O83401; -.
DR   OMA; O83401; LDDTYNA.
DR   BioCyc; TPAL243276:TP_0386-MON; -.
DR   BRENDA; 6.3.2.10; 142600.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0047480; F:UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-...; IEA:EC.
DR   GO; GO:0008766; F:UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-d...; IEA:InterPro.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007047; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   InterPro; IPR004101; Mur_ligase_C.
DR   InterPro; IPR013221; Mur_ligase_cen.
DR   InterPro; IPR000713; Mur_ligase_N.
DR   InterPro; IPR005863; UDP-N-AcMur-3pep_AlaAla_ligase.
DR   Gene3D; G3DSA:3.90.190.20; Mur_ligase_C; 1.
DR   Gene3D; G3DSA:3.40.1190.10; Mur_ligase_cen; 1.
DR   PANTHER; PTHR23135:SF3; MurF; 1.
DR   Pfam; PF01225; Mur_ligase; 1.
DR   Pfam; PF02875; Mur_ligase_C; 1.
DR   Pfam; PF08245; Mur_ligase_M; 1.
DR   TIGRFAMs; TIGR01143; murF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell cycle; Cell division; Cell shape;
KW   Cell wall biogenesis/degradation; Complete proteome; Cytoplasm;
KW   Ligase; Nucleotide-binding; Peptidoglycan synthesis.
FT   CHAIN         1    459       UDP-N-acetylmuramoyl-tripeptide--D-
FT                                alanyl-D-alanine ligase.
FT                                /FTId=PRO_0000101707.
FT   NP_BIND     121    127       ATP (Potential).
SQ   SEQUENCE   459 AA;  50235 MW;  23EA5571ED95F332 CRC64;
     MLLSFDEVCA AVQGARVCDA RGARGFDGVS FDSRAVVPRD LFIPLRGAHV DGHTFVEEAL
     QKGAVATLID QRYPHAGEYV AWCTRFGAAC IAVHDTLRAL QDLASFYCKK FPALIRIGIT
     GSSGKTTVKE MARAVFSERY RVVATPGNLN SEIGLPQSLF FVRAEHEVGI FELGMNRRGE
     MRTLAQILVP HYAIITNVGC AHVGILGTQQ AIAEEKKEIF SQFTEHSVGF VPDDAYRVFL
     SNIPYGRVVV YDQGGRGLAT EVIDEGLRGS RVLYQGRWIR VPLPGVHNAK NALAVIALAA
     QVGLPAEEIQ RGMERVKPPF GRSHVVCASL TFLLDCYNAN PDSMAAALHL CAHISAVSKV
     YVLGDMGELG VTAAEAHYRV CVLAAASDAR AVYVFGPEFC RAVRKVSWGR KRVYAFALEE
     LSALQETLDA QLRRGDFVLV KGSRSVALER LEPLLRKER
//