O83401 (MURF_TREPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
EC=6.3.2.10

Alternative name(s):
D-alanyl-D-alanine-adding enzyme
UDP-MurNAc-pentapeptide synthetase

Gene names

Name:	murF
Ordered Locus Names:	TP_0386

Organism

Treponema pallidum (strain Nichols) [Complete proteome] [HAMAP]

Taxonomic identifier

243276 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Treponema

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: EC UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase		PRO_0000101707
Regions
	Nucleotide binding	121 – 127	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O83401 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 23EA5571ED95F332	FASTA	459	50,235
	10 20 30 40 50 60 MLLSFDEVCA AVQGARVCDA RGARGFDGVS FDSRAVVPRD LFIPLRGAHV DGHTFVEEAL 70 80 90 100 110 120 QKGAVATLID QRYPHAGEYV AWCTRFGAAC IAVHDTLRAL QDLASFYCKK FPALIRIGIT 130 140 150 160 170 180 GSSGKTTVKE MARAVFSERY RVVATPGNLN SEIGLPQSLF FVRAEHEVGI FELGMNRRGE 190 200 210 220 230 240 MRTLAQILVP HYAIITNVGC AHVGILGTQQ AIAEEKKEIF SQFTEHSVGF VPDDAYRVFL 250 260 270 280 290 300 SNIPYGRVVV YDQGGRGLAT EVIDEGLRGS RVLYQGRWIR VPLPGVHNAK NALAVIALAA 310 320 330 340 350 360 QVGLPAEEIQ RGMERVKPPF GRSHVVCASL TFLLDCYNAN PDSMAAALHL CAHISAVSKV 370 380 390 400 410 420 YVLGDMGELG VTAAEAHYRV CVLAAASDAR AVYVFGPEFC RAVRKVSWGR KRVYAFALEE 430 440 450 LSALQETLDA QLRRGDFVLV KGSRSVALER LEPLLRKER

References

[1]

"Complete genome sequence of Treponema pallidum, the syphilis spirochete." Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G., Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A., Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D., Khalak H.G., Richardson D.L., Howell J.K. , Chidambaram M., Utterback T.R., McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A., Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O., Venter J.C. Science 281:375-388(1998) [PubMed: 9665876] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nichols.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000520 Genomic DNA. Translation: AAC65370.1.
PIR	D71331.
RefSeq	NP_218826.1. NC_000919.1.
3D structure databases
ProteinModelPortal	O83401.
ModBase	Search...
Protein-protein interaction databases
IntAct	O83401. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2611288.
GenomeReviews	Gene locus TP_0386 in contig AE000520_GR.
KEGG	tpa:TP0386.
NMPDR	fig|243276.1.peg.385.
PATRIC	20530781. VBITrePal57110_0410.
TIGR	TP_0386.
Phylogenomic databases
HOGENOM	HBG617121.
OMA	YAVIEMG.
ProtClustDB	CLSK218674.
Enzyme and pathway databases
BioCyc	TPAL243276:TP_0386-MONOMER.
Family and domain databases
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005863. UDP-N-AcMur-pentapeptide_synth. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
KO	K01929.
PANTHER	PTHR23135:SF3. MurF. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01143. MurF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURF_TREPA

Accession

Primary (citable) accession number: O83401

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program