O83386 (ISPE_TREPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	TP_0371

Organism

Treponema pallidum (strain Nichols) [Complete proteome] [HAMAP]

Taxonomic identifier

243276 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Treponema

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000189282
Regions
	Nucleotide binding	94 – 104	11	ATP Potential
Sites
	Active site	11	1	By similarity
	Active site	136	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O83386 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 410EC57910C3B81C	FASTA	291	31,592
	10 20 30 40 50 60 MQSLSLRAHA KVNMHLWVGA RRADGLHSIE SVMQRITLAD SLSLSRLDIP GRCEVCSPYM 70 80 90 100 110 120 ALPRENTLTR AYARFCQVTG VHDGVRVRVV KRIPAGSGLG GGSADAAALL CGLDTLFGTT 130 140 150 160 170 180 LSARVLREVA YSVGSDVPFF LASQAACVLG GGEQLVPLVP KTGYLGLLVW PGLHSGSAQA 190 200 210 220 230 240 YEDLDRLRAC GVHAADGEQY SLRGATALSA HYAQDCARWR FFNSLDAPVQ RRYPVVALAR 250 260 270 280 290 WDLARAGACF TAMSGSGSXV FGLYRDEEEL RRAHKLLAKR WCWCVRVRLC G

References

[1]

"Complete genome sequence of Treponema pallidum, the syphilis spirochete." Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G., Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A., Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D., Khalak H.G., Richardson D.L., Howell J.K. , Chidambaram M., Utterback T.R., McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A., Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O., Venter J.C. Science 281:375-388(1998) [PubMed: 9665876] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nichols.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000520 Genomic DNA. Translation: AAC65356.1.
PIR	B71333.
RefSeq	NP_218811.1. NC_000919.1.
3D structure databases
ProteinModelPortal	O83386.
ModBase	Search...
Protein-protein interaction databases
IntAct	O83386. 3 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2611620.
GenomeReviews	Gene locus TP_0371 in contig AE000520_GR.
KEGG	tpa:TP0371.
NMPDR	fig|243276.1.peg.370.
PATRIC	20530745. VBITrePal57110_0393.
TIGR	TP_0371.
Phylogenomic databases
HOGENOM	HBG734593.
OMA	GPFAKGL.
ProtClustDB	CLSK218665.
Enzyme and pathway databases
BioCyc	TPAL243276:TP_0371-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_TREPA

Accession

Primary (citable) accession number: O83386

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program