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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Treponema pallidum (strain Nichols)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi), Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei90ATP; via amide nitrogenUniRule annotation1
Metal bindingi183Magnesium; catalyticUniRule annotation1
Sitei184Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei213Proton acceptorUniRule annotation1
Binding sitei312SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi156 – 157ATPUniRule annotation2
Nucleotide bindingi182 – 185ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Synonyms:pfk
Ordered Locus Names:TP_0108
ORF Names:TPANIC_0108
OrganismiTreponema pallidum (strain Nichols)
Taxonomic identifieri243276 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
Proteomesi
  • UP000000811 Componenti: Chromosome
  • UP000014259 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004297131 – 461ATP-dependent 6-phosphofructokinaseAdd BLAST461

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243276.TP0108.

Structurei

3D structure databases

ProteinModelPortaliO83146.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni211 – 213Substrate bindingUniRule annotation3
Regioni256 – 258Substrate bindingUniRule annotation3
Regioni380 – 383Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
KOiK00850.
OMAiHSGFIAC.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

O83146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHLPKEYDF SIESLGESKI PSPIYLSHTL GDFIPNYVSD NEYISHELSA
60 70 80 90 100
RLGETVGPFT HKNLMERAGP RQKIFFNPHH VHAGIVTCGG LCPGLNDVIR
110 120 130 140 150
AIVRCLWGRY GVKRISGIRF GYKGLLPDYN FDILPLTPEV IDNCHKTGGS
160 170 180 190 200
LLGTSRGGGN RVVDIVDGIE RLNLHILFII GGDGSQKGAK EIADEIKHRN
210 220 230 240 250
LKISIIGIPK TVDNDISFVQ KSFGFDTAIV KATEAVAAAH MEARSQINGI
260 270 280 290 300
GLVKLMGRES GFIATYTAIA SHETNFVLIP EVSFDLDGPN GLLAHLEKRI
310 320 330 340 350
ALRKHAVLVV AEGAGQDLMV NADGVPSGDS QGGSLRVSSG TDASGNKRLA
360 370 380 390 400
DIGLFLKEKI GVYFKEKRIH INLKYIDPSY LIRSAVAAPI DSIYCERLGN
410 420 430 440 450
NAVHAAMCGK TKMIIGLVHN KFVHLPIDVV VCQRKHVNPE GSLWRDALDA
460
TGQPIVMKNI I
Length:461
Mass (Da):50,162
Last modified:November 1, 1998 - v1
Checksum:i6A902B8678CC8A54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000520 Genomic DNA. Translation: AAC26556.1.
CP004010 Genomic DNA. Translation: AGN75327.1.
PIRiA71366.
RefSeqiWP_010881557.1. NC_021490.2.

Genome annotation databases

EnsemblBacteriaiAAC26556; AAC26556; TP_0108.
AGN75327; AGN75327; TPANIC_0108.
GeneIDi2610848.
KEGGitpa:TP_0108.
tpw:TPANIC_0108.
PATRICi20530165. VBITrePal57110_0119.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000520 Genomic DNA. Translation: AAC26556.1.
CP004010 Genomic DNA. Translation: AGN75327.1.
PIRiA71366.
RefSeqiWP_010881557.1. NC_021490.2.

3D structure databases

ProteinModelPortaliO83146.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243276.TP0108.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC26556; AAC26556; TP_0108.
AGN75327; AGN75327; TPANIC_0108.
GeneIDi2610848.
KEGGitpa:TP_0108.
tpw:TPANIC_0108.
PATRICi20530165. VBITrePal57110_0119.

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
KOiK00850.
OMAiHSGFIAC.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA_TREPA
AccessioniPrimary (citable) accession number: O83146
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.