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Protein

Chaperone protein ClpB

Gene

clpB

Organism
Treponema pallidum (strain Nichols)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi206 – 213ATP 1By similarity8
Nucleotide bindingi609 – 616ATP 2By similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB
Gene namesi
Name:clpB
Ordered Locus Names:TP_0071
OrganismiTreponema pallidum (strain Nichols)
Taxonomic identifieri243276 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
Proteomesi
  • UP000000811 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001911971 – 878Chaperone protein ClpBAdd BLAST878

Proteomic databases

PRIDEiO83110.

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

Protein-protein interaction databases

IntActiO83110. 5 interactors.
STRINGi243276.TP0071.

Structurei

3D structure databases

ProteinModelPortaliO83110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 143N-terminalBy similarityAdd BLAST143
Regioni159 – 340NBD1By similarityAdd BLAST182
Regioni341 – 549LinkerBy similarityAdd BLAST209
Regioni559 – 783NBD2By similarityAdd BLAST225
Regioni784 – 878C-terminalBy similarityAdd BLAST95

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili391 – 525By similarityAdd BLAST135

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
KOiK03695.
OMAiPVERILM.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR017730. Chaperonin_ClpB.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR03346. chaperone_ClpB. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O83110-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTDRYTVKA SEALNDAISL AEAENHGQVE EEHLLHALLS QKDGIISPLI
60 70 80 90 100
EKIGAKPDFL YDELLQCLRR KPRVTGPAAQ TRCAPTLSKA CARAERLALK
110 120 130 140 150
NQDEYVSCEH LLLAISETDS NTARLLHSQG ITSKTISAAL KDIRGSKRVT
160 170 180 190 200
SQDPESTFQC LEKYCRDLTT LAREEKIDPV IGRDEEIRRV MQVLSRRTKN
210 220 230 240 250
NPVLIGEPGV GKTAIVEGLA RRIVSGDVPE SLKGKRLLSL DLGALVAGAK
260 270 280 290 300
FRGEFEERLK AVIEAVQKSD GGVILFIDEL HTLVGAGASE GSMDASNLLK
310 320 330 340 350
PALARGELRS IGATTLNEYR KYIEKDAALE RRFQQVYCVQ PTVEDTIAIL
360 370 380 390 400
RGLQEKYEVH HGVRIKDEAL VAATVLSDRY ITNRFLPDKA IDLVDEAASR
410 420 430 440 450
LKMEIESQPV ELDQVERKIL QLNIEKASLL KESDPASKER LEKLEKELAG
460 470 480 490 500
FLERRAAMQV QWQNEKGRIE ESRRYKEELE RLRIEETMFS REGDLNKAAE
510 520 530 540 550
LRYGKIPELE KKIMLLTAEV EKKSGLEGQL LREEVCEEDI AKIISMWTGI
560 570 580 590 600
PVSKMMASEQ QKYLQLESVL MQRVVGQDEA VRVISDAIRR NKAGLSDTRR
610 620 630 640 650
PLGSFLCVGP TGVGKTELAR TLADFLFNDE RALTRIDMSE YMEKHAISRL
660 670 680 690 700
IGAPPGYVGY DEGGQLTEAV RRRPYSVLLF DEVEKAHQDV FNIFLQILDD
710 720 730 740 750
GRLTDGQGRV VDFRNTIIIM TSNIGSEHIL SARESRTHTS DLPVPETQST
760 770 780 790 800
EEQTLPEQIR GLLHTYFRPE FLNRIDEVLI FKRLTRKHIR LITDIQLQMV
810 820 830 840 850
VERLESRHIK LRVRDAAKAY LAERGYDDTF GARPLKRAIQ TELENALARE
860 870
ILSGRFRGGS TIVVDMCKDA LCFTEQTS
Length:878
Mass (Da):98,981
Last modified:November 1, 1998 - v1
Checksum:i7D9E7419E42A2202
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000520 Genomic DNA. Translation: AAC65062.1.
PIRiG71371.
RefSeqiWP_010881520.1. NC_021490.2.

Genome annotation databases

EnsemblBacteriaiAAC65062; AAC65062; TP_0071.
GeneIDi2611222.
KEGGitpa:TP_0071.
PATRICi20530079. VBITrePal57110_0076.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000520 Genomic DNA. Translation: AAC65062.1.
PIRiG71371.
RefSeqiWP_010881520.1. NC_021490.2.

3D structure databases

ProteinModelPortaliO83110.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO83110. 5 interactors.
STRINGi243276.TP0071.

Proteomic databases

PRIDEiO83110.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC65062; AAC65062; TP_0071.
GeneIDi2611222.
KEGGitpa:TP_0071.
PATRICi20530079. VBITrePal57110_0076.

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
KOiK03695.
OMAiPVERILM.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR017730. Chaperonin_ClpB.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR03346. chaperone_ClpB. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLPB_TREPA
AccessioniPrimary (citable) accession number: O83110
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.