Reviewed, UniProtKB/Swiss-Prot O83051 (GYRA_TREPA)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	TP_0005

Organism

Treponema pallidum [Complete proteome] [HAMAP]

Taxonomic identifier

160 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Treponema

Protein attributes

Sequence length	813 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	O83925	2	EBI-1198241,EBI-1198939
	O83927	2	EBI-1198241,EBI-1198947
flaB3	P21992	2	EBI-1198241,EBI-1198854
flgD	O07893	2	EBI-1198241,EBI-1198815
fliE	O83413	2	EBI-1198241,EBI-1198218

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 813	813	DNA gyrase subunit A		PRO_0000145271
Sites
	Active site	126	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O83051-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 1F6A3AE08C43ECC4	FASTA	813	89,926
	10 20 30 40 50 60 MEEISTPEGG VLVPISIETE VKRAYIDYSM SVIVSRALPD VRDGLKPVHR RILYAMEEKG 70 80 90 100 110 120 LRFSGPTRKC AKIVGDVLGS FHPHGDASVY DALVRLGQDF SLRYPVIHPQ GNFGTIGGDP 130 140 150 160 170 180 PAAYRYTEAK MARIAESMVE DIKKETVSFV PNFDDSDVEP TVLPGRFPFL LANGSSGIAV 190 200 210 220 230 240 GMTTNMPPHN LREIAAAISA YIENPNLSIQ ELCDCINGPD FPTGGIIFGK NGIRQSYETG 250 260 270 280 290 300 RGKIVVRARF TIETDSKGRD TIIFTEVPYQ VNTTMLVMRI GELARAKVIE GIANVNDETS 310 320 330 340 350 360 DRTGLRIVVE LKKGTPAQVV LNHLFAKTPL QSSFNVINLA LVEGRPRMLT LKDLVRYFVE 370 380 390 400 410 420 HRVDVVTRRA HFELRKAQER IHLVRALIRA LDAIDKIITL IRHSQNTELA KQRLREQFDF 430 440 450 460 470 480 DNVQAQAIVD MQMKRLTGLE VESLRTELKD LTELISSLEE LLTSPQKVLG VVKKETRDIA 490 500 510 520 530 540 DMFGDDRRTD IVSNEIEYLD VEDFIQKEEM VILISHLGYI KRVPVSAYRN QNRGGKGSSS 550 560 570 580 590 600 ANLAAHDFIS QIFTASTHDY VMFVTSRGRA YWLKVYGIPE SGRANRGSHI KSLLMVATDE 610 620 630 640 650 660 EITAIVSLRE FSNKSYVFMA TARGVVKKVT TDNFVNAKTR GIIALKLSGG DTLVSAVLVQ 670 680 690 700 710 720 DEDEVMLITR QGKALRMSGR EVREMGRNSS GVIGIKLTSE DLVAGVLRVS EQRKVLIMTE 730 740 750 760 770 780 NGYGKRVSFS EFSVHGRGTA GQKIYTQTDR KGAIIGALAV LDTDECMCIT GQGKTIRVDV 790 800 810 CAISVLGRGA QGVRVLDIEP SDLVVGLSCV MQG

References

[1]

"Complete genome sequence of Treponema pallidum, the syphilis spirochete." Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G., Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A., Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D., Khalak H.G., Richardson D.L., Howell J.K. , Chidambaram M., Utterback T.R., McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A., Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O., Venter J.C. Science 281:375-388(1998) [PubMed: 9665876] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nichols.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000520 Genomic DNA. Translation: AAC64998.1.
PIR	D71378.
RefSeq	NP_218446.1.
3D structure databases
HSSP	HSSP built from PDB template 1AB4 based on UniProtKB P09097.
ModBase	Search...
Protein-protein interaction databases
IntAct	O83051. 9 interactions.
Genome annotation databases
GeneID	2611298.
GenomeReviews	Gene locus TP_0005 in contig AE000520_GR.
KEGG	tpa:TP0005.
NMPDR	fig|243276.1.peg.5.
TIGR	TP_0005.
Phylogenomic databases
HOGENOM	O83051.
OMA	ELGNDWN.
Enzyme and pathway databases
BioCyc	TPAL243276:TP_0005-MON.
BRENDA	5.99.1.3. 142600.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_TREPA

Accession

Primary (citable) accession number: O83051

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)