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Protein

Reaction center protein L chain

Gene

pufL

Organism
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi154 – 1541Magnesium (bacteriochlorophyll b axial ligand)By similarity
Metal bindingi174 – 1741Magnesium (bacteriochlorophyll b axial ligand)By similarity
Metal bindingi191 – 1911IronBy similarity
Binding sitei217 – 2171Quinone BBy similarity
Metal bindingi231 – 2311IronBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Bacteriochlorophyll, Chlorophyll, Chromophore, Iron, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Reaction center protein L chain
Alternative name(s):
Photosynthetic reaction center L subunit
Gene namesi
Name:pufL
Ordered Locus Names:RPA1527
OrganismiRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Taxonomic identifieri258594 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
Proteomesi
  • UP000001426 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei30 – 5223HelicalSequence analysisAdd
BLAST
Transmembranei84 – 10623HelicalSequence analysisAdd
BLAST
Transmembranei113 – 13523HelicalSequence analysisAdd
BLAST
Transmembranei173 – 19523HelicalSequence analysisAdd
BLAST
Transmembranei233 – 25523HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Reaction center

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 277276Reaction center protein L chainPRO_0000090405Add
BLAST

Interactioni

Subunit structurei

Reaction center is composed of four bacteriochlorophylls, two bacteriopheophytins, two ubiquinones, one iron, and three highly hydrophobic polypeptide chains (designated L, M, and H).

Protein-protein interaction databases

STRINGi258594.RPA1527.

Structurei

3D structure databases

ProteinModelPortaliO83005.
SMRiO83005. Positions 2-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105VGZ. Bacteria.
ENOG410XVH6. LUCA.
HOGENOMiHOG000230362.
KOiK08928.
OMAiNLWQISI.
OrthoDBiEOG6KDKNT.
PhylomeDBiO83005.

Family and domain databases

Gene3Di1.20.85.10. 2 hits.
InterProiIPR005871. Photo_RC_L.
IPR000484. Photo_RC_L/M.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01157. pufL. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O83005-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMLSFEKKY RVRGGTLIGG DLFDFWVGPF YVGIFGVMTV FFALIGIALI
60 70 80 90 100
AWNTALGPTW NLWQISVNPP DAKYGLGFAP LAEGGIWQWV SICATGAFVT
110 120 130 140 150
WALREVEICR KLGIGFHVPF AFSFAIFAYV TLVVIRPVLM GSWSYGFPYG
160 170 180 190 200
IFTHLDWVSN TGYSYGQFHY NPAHMIAITF FFTTCLALAL HGGLVLSALN
210 220 230 240 250
PDRGEPVKSP EHENTVFRDL VGYSIGTIGI HRLGLFLALS AVFFSAVCMI
260 270
ISGPVLAEGG SWPDWWNWWR NLPIWNP
Length:277
Mass (Da):30,837
Last modified:January 23, 2007 - v4
Checksum:i956B6710FC595013
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 341I → F in BAA33001 (Ref. 1) Curated
Sequence conflicti38 – 392MT → TA in BAA33001 (Ref. 1) Curated
Sequence conflicti42 – 421F → C in BAA33001 (Ref. 1) Curated
Sequence conflicti45 – 473IGI → MGT in BAA33001 (Ref. 1) Curated
Sequence conflicti51 – 511A → I in BAA33001 (Ref. 1) Curated
Sequence conflicti91 – 933SIC → TIF in BAA33001 (Ref. 1) Curated
Sequence conflicti99 – 1002VT → CS in BAA33001 (Ref. 1) Curated
Sequence conflicti116 – 1161F → Y in BAA33001 (Ref. 1) Curated
Sequence conflicti130 – 1301V → L in BAA33001 (Ref. 1) Curated
Sequence conflicti164 – 1641S → Q in BAA33001 (Ref. 1) Curated
Sequence conflicti170 – 1701Y → W in BAA33001 (Ref. 1) Curated
Sequence conflicti173 – 1731A → G in BAA33001 (Ref. 1) Curated
Sequence conflicti199 – 1991L → I in BAA33001 (Ref. 1) Curated
Sequence conflicti233 – 2331L → V in BAA33001 (Ref. 1) Curated
Sequence conflicti244 – 2441F → W in BAA33001 (Ref. 1) Curated
Sequence conflicti250 – 2501I → L in BAA33001 (Ref. 1) Curated
Sequence conflicti257 – 2571A → P in BAA33001 (Ref. 1) Curated
Sequence conflicti264 – 2641D → E in BAA33001 (Ref. 1) Curated
Sequence conflicti267 – 2671N → E in BAA33001 (Ref. 1) Curated
Sequence conflicti271 – 2722NL → RI in BAA33001 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015977 mRNA. Translation: BAA33001.1.
BX572597 Genomic DNA. Translation: CAE26969.1.
RefSeqiWP_011157088.1. NC_005296.1.

Genome annotation databases

EnsemblBacteriaiCAE26969; CAE26969; RPA1527.
KEGGirpa:RPA1527.
PATRICi23287297. VBIRhoPal84835_1588.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015977 mRNA. Translation: BAA33001.1.
BX572597 Genomic DNA. Translation: CAE26969.1.
RefSeqiWP_011157088.1. NC_005296.1.

3D structure databases

ProteinModelPortaliO83005.
SMRiO83005. Positions 2-275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi258594.RPA1527.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE26969; CAE26969; RPA1527.
KEGGirpa:RPA1527.
PATRICi23287297. VBIRhoPal84835_1588.

Phylogenomic databases

eggNOGiENOG4105VGZ. Bacteria.
ENOG410XVH6. LUCA.
HOGENOMiHOG000230362.
KOiK08928.
OMAiNLWQISI.
OrthoDBiEOG6KDKNT.
PhylomeDBiO83005.

Family and domain databases

Gene3Di1.20.85.10. 2 hits.
InterProiIPR005871. Photo_RC_L.
IPR000484. Photo_RC_L/M.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01157. pufL. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Use of gyrB gene, pufL and pufM genes and 16S rRNA sequence analysis to investigate phylogeny of photosynthetic bacteria."
    Hamada T.
    Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HMD002.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-98 / CGA009.

Entry informationi

Entry nameiRCEL_RHOPA
AccessioniPrimary (citable) accession number: O83005
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: November 11, 2015
This is version 109 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.