Reviewed, UniProtKB/Swiss-Prot O82859 (BCSA2_ACEXY)

Names and origin

Protein names

Recommended name:
    Cellulose synthase catalytic subunit [UDP-forming]
    EC=2.4.1.12

Gene names

Name:

bcsA

Organism

Acetobacter xylinus (Gluconacetobacter xylinus)

Taxonomic identifier

28448 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconacetobacter

Protein attributes

Sequence length	756 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of cellulose synthase. It polymerizes uridine 5'-diphosphate glucose to cellulose. The thick cellulosic mats generated by this enzyme probably provide a specialized protective environment to the bacterium By similarity.
Catalytic activity	UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by bis-(3'-5') cyclic diguanylic acid (c-di-GMP).
Pathway	Glycan metabolism; bacterial cellulose biosynthesis.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Domain	There are two conserved domains in the globular part of the catalytic subunit: the N-terminal domain (domain A) contains the conserved DXD motif and is possibly involved in catalysis and substrate binding. The C-terminal domain (domain B) contains the QXXRW motif and is present only in processive glycosyl transferases. It could be involved in the processivity function of the enzyme, possibly required for holding the growing glycan chain in the active site.
Sequence similarities	Belongs to the glycosyltransferase 2 family.

Ontologies

Keywords
Biological process	Cellulose biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	c-di-GMP
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	UDP-glucose metabolic process Inferred from electronic annotation. Source: InterPro cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to plasma membrane Inferred from electronic annotation. Source: InterPro
Molecular function	cellulose synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 756	756	Cellulose synthase catalytic subunit [UDP-forming]		PRO_0000059263
Regions
	Transmembrane	27 – 47	21	Potential
	Transmembrane	49 – 69	21	Potential
	Transmembrane	106 – 126	21	Potential
	Transmembrane	167 – 187	21	Potential
	Transmembrane	409 – 429	21	Potential
	Transmembrane	432 – 452	21	Potential
	Transmembrane	470 – 490	21	Potential
	Transmembrane	517 – 537	21	Potential
	Transmembrane	551 – 571	21	Potential
	Region	147 – 242	96	Catalytic subdomain A
	Region	319 – 379	61	Catalytic subdomain B
Sites
	Active site	189	1	Potential
	Active site	335	1	Potential
	Binding site	238	1	Substrate Potential
	Binding site	240	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	O82859-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 6954F39A25E73B0A	FASTA	756	84,562
	10 20 30 40 50 60 MSEVQSPVPT ESRLGRISNK ILSLRGASYI VGALGLCALI AATTVTLNNN EQLIVAAVCV 70 80 90 100 110 120 VIFFVVGRGK SRRTQIFLEV LSALVSLRYL TWRLTETLDF NTWIQGILGV ILLMAELYAL 130 140 150 160 170 180 YMLFLSYFQT IQPLHRAPLP LPDNVDDWPT VDIFIPTYDE QLSIVRLTVL GALGIDWPPD 190 200 210 220 230 240 KVNVYILDDG VRPEFEQFAK DCGALYIGRV DVDSAHAKAG NLNHAIKRTS GDYILILDCD 250 260 270 280 290 300 HIPTRAFLQI AMGWMVADRK IALMQTPHHF YSPDPFQRNL AVGYRTPPEG NLFYGVIQDG 310 320 330 340 350 360 NDFWDATFFC GSCAILRREA IESIGGFAVE TVTEDAHTAL RMQRRGWSTA YLRIPVASGL 370 380 390 400 410 420 ATERLTTHIG QRMRWARGMI QIFRVDNPML GRGLKLGQRL CYLSAMTSFF FAIPRVIFLA 430 440 450 460 470 480 SPLAFLFAGQ NIIAAAPLAV AAYALPHMFH SIATAAKVNK GWRYSFWSEV YETTMALFLV 490 500 510 520 530 540 RVTIVTLLFP SKGKFNVTEK GGVLEEEEFD LGATYPNIIF ATIMMGGLLI GLFELIVRFN 550 560 570 580 590 600 QLDVIARNAY LLNCAWALIS LIILFAAIAV GRETKQVRYN HRVEAHIPVT VYDAPAEGQP 610 620 630 640 650 660 HTYYNATHGM TQDVSMGGVA VHIPLPDVTT GPVKKRIHAV LDGEEIDIPA TMLRCTNGKA 670 680 690 700 710 720 VFTWDNNDLD TERDIVRFVF GRADAWLQWN NYEDDRPLRS LWSLLLSIKA LFRKKGKIMA 730 740 750 NSRPKKKPLA LPVERREPTT IHSGQTQEGK ISRAAS

References

[1]

"Control of expression by the cellulose synthase (bcsA) promoter region from Acetobacter xylinum BPR 2001." Nakai T., Moriya A., Tonouchi N., Tsuchida T., Yoshinaga F., Horinouchi S., Sone Y., Mori H., Sakai F., Hayashi T. Gene 213:93-100(1998) [PubMed: 9630539] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 700178 / BPR 2001 / JCM 9730 / LMG 18788.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB010645 Genomic DNA. Translation: BAA31463.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Family and domain databases
InterPro	IPR003919. Cell_synth_A. IPR017480. Cellulose_synth_catalytic. IPR001173. Glyco_trans_2. IPR009875. PilZ. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. PF07238. PilZ. 1 hit. [Graphical view]
PRINTS	PR01439. CELLSNTHASEA.
TIGRFAMs	TIGR03030. CelA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BCSA2_ACEXY

Accession

Primary (citable) accession number: O82859

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)