O82839 (O82839_BACSP) Unreviewed, UniProtKB/TrEMBL
Last modified
July 27, 2011.
Version 53.
History...
Names·Attributes·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
Names·Attributes·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize orderNames and origin
| Protein names | Submitted name: Amylase EMBL BAA32431.1 |
| Organism | Bacillus sp. EMBL BAA32431.1 |
| Taxonomic identifier | 1409 [NCBI] |
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 516 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
Ontologies
| Keywords | |
|---|---|
| Ligand | Calcium PDB 1W9X PDB 2DIE Metal-binding PDB 1W9X PDB 2DIE |
| Technical term | 3D-structure PDB 1W9X PDB 2DIE |
| Gene Ontology (GO) | |
| Biological process | carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compoundsInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Sites | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Metal binding | 137 | 1 | Calcium 1 PDB 1W9X PDB 2DIE | ||||||
| Metal binding | 194 | 1 | Calcium 2 PDB 1W9X PDB 2DIE | ||||||
| Metal binding | 217 | 1 | Calcium 2; via carbonyl oxygen PDB 1W9X PDB 2DIE | ||||||
| Metal binding | 219 | 1 | Calcium 2 PDB 1W9X PDB 2DIE | ||||||
| Metal binding | 230 | 1 | Calcium 1 PDB 1W9X PDB 2DIE | ||||||
| Metal binding | 236 | 1 | Calcium 1 PDB 1W9X PDB 2DIE | ||||||
| Metal binding | 238 | 1 | Calcium 2 PDB 1W9X PDB 2DIE | ||||||
| Metal binding | 240 | 1 | Calcium 2 PDB 1W9X | ||||||
| Metal binding | 271 | 1 | Calcium 1; via carbonyl oxygen PDB 1W9X PDB 2DIE | ||||||
| Metal binding | 336 | 1 | Calcium 3; via carbonyl oxygen PDB 1W9X PDB 2DIE | ||||||
| Metal binding | 338 | 1 | Calcium 3; via carbonyl oxygen PDB 2DIE | ||||||
| Metal binding | 439 | 1 | Calcium 3; via carbonyl oxygen PDB 1W9X PDB 2DIE | ||||||
| Metal binding | 440 | 1 | Calcium 3 PDB 2DIE | ||||||
| Metal binding | 463 | 1 | Calcium 3 PDB 1W9X PDB 2DIE | ||||||
| Binding site | 197 | 1 | Glucose PDB 1W9X | ||||||
| Binding site | 225 | 1 | Glucose PDB 1W9X | ||||||
| Binding site | 234 | 1 | Glucose PDB 1W9X | ||||||
| Binding site | 270 | 1 | Glucose PDB 1W9X | ||||||
Sequences
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References
| [1] | "Improved thermostability of a Bacillus alpha-amylase by deletion of an arginine-glycine residue is caused by enhanced calcium binding." Igarashi K., Hatada Y., Ikawa K., Araki H., Ozawa T., Kobayashi T., Ozaki K., Ito S. Biochem. Biophys. Res. Commun. 248:372-377(1998) [PubMed: 9675143] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: KSM-1378 EMBL BAA32431.1. |
| [2] | "Ancestral sequence evolutionary trace and crystal structure analyses of alkaline alpha-amylase from Bacillus sp. KSM-1378 to clarify the alkaline adaptation process of proteins." Shirai T., Igarashi K., Ozawa T., Hagihara H., Kobayashi T., Ozaki K., Ito S. Proteins 66:600-610(2007) [PubMed: 17154418] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 32-516 IN COMPLEX WITH CALCIUM. |
| [3] | "Structure of a Bacillus halmapalus family 13 alpha-amylase, BHA, in complex with an acarbose-derived nonasaccharide at 2.1 A resolution." Davies G.J., Brzozowski A.M., Dauter Z., Rasmussen M.D., Borchert T.V., Wilson K.S. Acta Crystallogr. D Biol. Crystallogr. 61:190-193(2005) [PubMed: 15681870] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 36-515 IN COMPLEX WITH CALCIUM AND GLUCOSE. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | AB008763 Genomic DNA. Translation: BAA32431.1. | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | O82839. | ||||||||||||||||||
| SMR | O82839. Positions 36-516. | ||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||
Protein family/group databases | |||||||||||||||||||
| CAZy | GH13. Glycoside Hydrolase Family 13. | ||||||||||||||||||
Protocols and materials databases | |||||||||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR013776. A-amylase_thermo. IPR015237. Alpha-amylase_C_pro. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view] | ||||||||||||||||||
| Gene3D | G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. | ||||||||||||||||||
| PANTHER | PTHR10357. Alpha_amylase. 1 hit. | ||||||||||||||||||
| Pfam | PF00128. Alpha-amylase. 1 hit. PF09154. DUF1939. 1 hit. [Graphical view] | ||||||||||||||||||
| PIRSF | PIRSF001021. Alph-amls_thrmst. 1 hit. | ||||||||||||||||||
| SMART | SM00642. Aamy. 1 hit. [Graphical view] | ||||||||||||||||||
| SUPFAM | SSF51445. Glyco_hydro_cat. 1 hit. | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Entry information
| Entry name | O82839_BACSP | ||||||||
| Accession | Primary (citable) accession number: O82839 | ||||||||
| Entry history |
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| Entry status | Unreviewed (UniProtKB/TrEMBL) | ||||||||

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