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Protein
Submitted name:

Amylase

Gene
N/A
Organism
Bacillus sp.
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi137 – 1371Calcium 1Combined sources
Metal bindingi194 – 1941Calcium 2Combined sources
Binding sitei197 – 1971GlucoseCombined sources
Metal bindingi217 – 2171Calcium 2; via carbonyl oxygenCombined sources
Metal bindingi219 – 2191Calcium 2Combined sources
Binding sitei225 – 2251GlucoseCombined sources
Metal bindingi230 – 2301Calcium 1Combined sources
Binding sitei234 – 2341GlucoseCombined sources
Metal bindingi236 – 2361Calcium 1Combined sources
Metal bindingi238 – 2381Calcium 2Combined sources
Metal bindingi240 – 2401Calcium 2Combined sources
Active sitei267 – 2671NucleophileUniRule annotation
Binding sitei270 – 2701GlucoseCombined sources
Metal bindingi271 – 2711Calcium 1; via carbonyl oxygenCombined sources
Active sitei297 – 2971Proton donorUniRule annotation
Metal bindingi336 – 3361Calcium 3; via carbonyl oxygenCombined sources
Metal bindingi336 – 3361Calcium 4; via carbonyl oxygenCombined sources
Metal bindingi338 – 3381Calcium 3; via carbonyl oxygenCombined sources
Metal bindingi439 – 4391Calcium 3; via carbonyl oxygenCombined sources
Metal bindingi439 – 4391Calcium 4; via carbonyl oxygenCombined sources
Metal bindingi440 – 4401Calcium 3Combined sources
Metal bindingi463 – 4631Calcium 3Combined sources
Metal bindingi463 – 4631Calcium 4Combined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

CalciumCombined sources, Metal-bindingCombined sources

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Submitted name:
AmylaseImported
OrganismiBacillus sp.Imported
Taxonomic identifieri1409 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence analysisAdd
BLAST
Chaini32 – 516485Sequence analysisPRO_5004160241Add
BLAST

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1W9XX-ray2.10A36-515[»]
2DIEX-ray2.10A32-516[»]
ProteinModelPortaliO82839.
SMRiO82839. Positions 36-516.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO82839.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 424387AamyInterPro annotationAdd
BLAST

Keywords - Domaini

SignalSequence analysis

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013776. A-amylase_thermo.
IPR015237. Alpha-amylase_C_pro.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09154. DUF1939. 1 hit.
[Graphical view]
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

O82839-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLHNRIISV LLTLLLAVAV LFPYMTEPAQ AHHNGTNGTM MQYFEWHLPN
60 70 80 90 100
DGNHWNRLRD DAANLKSKGI TAVWIPPAWK GTSQNDVGYG AYDLYDLGEF
110 120 130 140 150
NQKGTVRTKY GTRSQLQGAV TSLKNNGIQV YGDVVMNHKG GADGTEMVNA
160 170 180 190 200
VEVNRSNRNQ EISGEYTIEA WTKFDFPGRG NTHSNFKWRW YHFDGTDWDQ
210 220 230 240 250
SRQLQNKIYK FRGTGKAWDW EVDIENGNYD YLMYADIDMD HPEVINELRN
260 270 280 290 300
WGVWYTNTLN LDGFRIDAVK HIKYSYTRDW LTHVRNTTGK PMFAVAEFWK
310 320 330 340 350
NDLAAIENYL NKTSWNHSVF DVPLHYNLYN ASNSGGYFDM RNILNGSVVQ
360 370 380 390 400
KHPIHAVTFV DNHDSQPGEA LESFVQSWFK PLAYALILTR EQGYPSVFYG
410 420 430 440 450
DYYGIPTHGV PSMKSKIDPL LQARQTYAYG TQHDYFDHHD IIGWTREGDS
460 470 480 490 500
SHPNSGLATI MSDGPGGNKW MYVGKHKAGQ VWRDITGNRS GTVTINADGW
510
GNFTVNGGAV SVWVKQ
Length:516
Mass (Da):58,842
Last modified:November 1, 1998 - v1
Checksum:iD90A8C90ECC182F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008763 Genomic DNA. Translation: BAA32431.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008763 Genomic DNA. Translation: BAA32431.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1W9XX-ray2.10A36-515[»]
2DIEX-ray2.10A32-516[»]
ProteinModelPortaliO82839.
SMRiO82839. Positions 36-516.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO82839.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013776. A-amylase_thermo.
IPR015237. Alpha-amylase_C_pro.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09154. DUF1939. 1 hit.
[Graphical view]
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiO82839_BACSP
AccessioniPrimary (citable) accession number: O82839
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.