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Protein

High-affinity nitrate transporter 2.1

Gene

NRT2.1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in nitrate transport, but does not seem to be able to mediate transport by its own. Acts as a dual component transporter with NTR3.1. Acts as a repressor of lateral root initiation under high sucrose/low nitrate conditions.6 Publications

GO - Molecular functioni

  • nitrate transmembrane transporter activity Source: TAIR

GO - Biological processi

  • lateral root development Source: TAIR
  • nitrate assimilation Source: UniProtKB-KW
  • nitrate transport Source: CACAO
  • response to nitrate Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Nitrate assimilation

Protein family/group databases

TCDBi2.A.1.8.12. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity nitrate transporter 2.1
Short name:
AtNRT2:1
Alternative name(s):
Protein ACH1
Protein LATERAL ROOT INITIATION 1
Gene namesi
Name:NRT2.1
Synonyms:ACH1, LIN1, NRT2;1AT
Ordered Locus Names:At1g08090
ORF Names:T6D22.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G08090.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei69 – 8921HelicalSequence analysisAdd
BLAST
Transmembranei105 – 12521HelicalSequence analysisAdd
BLAST
Transmembranei129 – 14921HelicalSequence analysisAdd
BLAST
Transmembranei151 – 17121HelicalSequence analysisAdd
BLAST
Transmembranei193 – 21321HelicalSequence analysisAdd
BLAST
Transmembranei226 – 24621HelicalSequence analysisAdd
BLAST
Transmembranei271 – 29020HelicalSequence analysisAdd
BLAST
Transmembranei310 – 33021HelicalSequence analysisAdd
BLAST
Transmembranei352 – 37221HelicalSequence analysisAdd
BLAST
Transmembranei383 – 40321HelicalSequence analysisAdd
BLAST
Transmembranei410 – 43021HelicalSequence analysisAdd
BLAST
Transmembranei441 – 46121HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Impaired in the high-affinity nitrate transport. Reduced growth on low-nitrate media. Reduced nitrate uptake and increased lateral root initiation.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi119 – 1191G → R in lin1; reduced nitrate uptake and increased lateral root initiation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530High-affinity nitrate transporter 2.1PRO_0000400098Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei521 – 5211PhosphothreonineBy similarity

Post-translational modificationi

Might be subject to partial proteolysis at the C-terminus.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO82811.
PRIDEiO82811.

PTM databases

iPTMnetiO82811.

Expressioni

Tissue specificityi

Root cortical and epidermal cells. Not expressed in new lateral root Primordia. Detected in shoots.4 Publications

Inductioni

Up-regulated at transcript level by light and sucrose, and transiently by nitrate, but no changes at protein level. Down-regulated by ammonium, amino acids and N-metabolites resulting from nitrate reduction. Induced by sudden N starvation and by growth on low nitrate concentration. Circadian-regulation. Expression increases during the light phase and decreases during the dark phase.8 Publications

Gene expression databases

GenevisibleiO82811. AT.

Interactioni

Subunit structurei

Monomer. Heterotetramer composed of two NRT2.1 and two NRT3.1. Interacts with NRT3.1.2 Publications

Protein-protein interaction databases

BioGridi22568. 1 interaction.
IntActiO82811. 1 interaction.
STRINGi3702.AT1G08090.1.

Structurei

3D structure databases

ProteinModelPortaliO82811.
SMRiO82811. Positions 95-289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IE1F. Eukaryota.
COG2223. LUCA.
HOGENOMiHOG000196276.
InParanoidiO82811.
KOiK02575.
OMAiFFSTSHF.
PhylomeDBiO82811.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.

Sequencei

Sequence statusi: Complete.

O82811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDSTGEPGS SMHGVTGREQ SFAFSVQSPI VHTDKTAKFD LPVDTEHKAT
60 70 80 90 100
VFKLFSFAKP HMRTFHLSWI SFSTCFVSTF AAAPLVPIIR ENLNLTKQDI
110 120 130 140 150
GNAGVASVSG SIFSRLVMGA VCDLLGPRYG CAFLVMLSAP TVFSMSFVSD
160 170 180 190 200
AAGFITVRFM IGFCLATFVS CQYWMSTMFN SQIIGLVNGT AAGWGNMGGG
210 220 230 240 250
ITQLLMPIVY EIIRRCGSTA FTAWRIAFFV PGWLHIIMGI LVLNLGQDLP
260 270 280 290 300
DGNRATLEKA GEVAKDKFGK ILWYAVTNYR TWIFVLLYGY SMGVELSTDN
310 320 330 340 350
VIAEYFFDRF HLKLHTAGLI AACFGMANFF ARPAGGYASD FAAKYFGMRG
360 370 380 390 400
RLWTLWIIQT AGGLFCVWLG RANTLVTAVV AMVLFSMGAQ AACGATFAIV
410 420 430 440 450
PFVSRRALGI ISGLTGAGGN FGSGLTQLLF FSTSHFTTEQ GLTWMGVMIV
460 470 480 490 500
ACTLPVTLVH FPQWGSMFLP PSTDPVKGTE AHYYGSEWNE QEKQKNMHQG
510 520 530
SLRFAENAKS EGGRRVRSAA TPPENTPNNV
Length:530
Mass (Da):57,710
Last modified:November 1, 1998 - v1
Checksum:iA40F435039635090
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97058 mRNA. Translation: CAB09794.1.
AF019748 mRNA. Translation: AAC35883.1.
AF093754 mRNA. Translation: AAC64170.1.
AC026875 Genomic DNA. Translation: AAF79826.1.
CP002684 Genomic DNA. Translation: AEE28241.1.
PIRiT51836.
RefSeqiNP_172288.1. NM_100684.2.
UniGeneiAt.5407.

Genome annotation databases

EnsemblPlantsiAT1G08090.1; AT1G08090.1; AT1G08090.
GeneIDi837327.
GrameneiAT1G08090.1; AT1G08090.1; AT1G08090.
KEGGiath:AT1G08090.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97058 mRNA. Translation: CAB09794.1.
AF019748 mRNA. Translation: AAC35883.1.
AF093754 mRNA. Translation: AAC64170.1.
AC026875 Genomic DNA. Translation: AAF79826.1.
CP002684 Genomic DNA. Translation: AEE28241.1.
PIRiT51836.
RefSeqiNP_172288.1. NM_100684.2.
UniGeneiAt.5407.

3D structure databases

ProteinModelPortaliO82811.
SMRiO82811. Positions 95-289.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22568. 1 interaction.
IntActiO82811. 1 interaction.
STRINGi3702.AT1G08090.1.

Protein family/group databases

TCDBi2.A.1.8.12. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiO82811.

Proteomic databases

PaxDbiO82811.
PRIDEiO82811.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G08090.1; AT1G08090.1; AT1G08090.
GeneIDi837327.
GrameneiAT1G08090.1; AT1G08090.1; AT1G08090.
KEGGiath:AT1G08090.

Organism-specific databases

TAIRiAT1G08090.

Phylogenomic databases

eggNOGiENOG410IE1F. Eukaryota.
COG2223. LUCA.
HOGENOMiHOG000196276.
InParanoidiO82811.
KOiK02575.
OMAiFFSTSHF.
PhylomeDBiO82811.

Miscellaneous databases

PROiO82811.

Gene expression databases

GenevisibleiO82811. AT.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterisation of the NRT2 high affinity nitrate transporters from Arabidopsis."
    Zhang H., Forde B.G.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Landsberg erecta.
  2. "Regulation of a putative high-affinity nitrate transporter (Nrt2;1At) in roots of Arabidopsis thaliana."
    Zhuo D., Okamoto M., Vidmar J.J., Glass A.D.
    Plant J. 17:563-568(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY NITRATE AND AMMONIUM, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  3. "Nitrate transport: a key step in nitrate assimilation."
    Daniel-Vedele F., Filleur S., Caboche M.
    Curr. Opin. Plant Biol. 1:235-239(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Landsberg erecta.
  4. "Expression analysis of a high-affinity nitrate transporter isolated from Arabidopsis thaliana by differential display."
    Filleur S., Daniel-Vedele F.
    Planta 207:461-469(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Landsberg erecta.
  5. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  6. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  7. "Molecular and functional regulation of two NO3- uptake systems by N- and C-status of Arabidopsis plants."
    Lejay L., Tillard P., Lepetit M., Olive F., Filleur S., Daniel-Vedele F., Gojon A.
    Plant J. 18:509-519(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY NITRATE AND LIGHT.
  8. "An arabidopsis T-DNA mutant affected in Nrt2 genes is impaired in nitrate uptake."
    Filleur S., Dorbe M.F., Cerezo M., Orsel M., Granier F., Gojon A., Daniel-Vedele F.
    FEBS Lett. 489:220-224(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  9. "Major alterations of the regulation of root NO(3)(-) uptake are associated with the mutation of Nrt2.1 and Nrt2.2 genes in Arabidopsis."
    Cerezo M., Tillard P., Filleur S., Munos S., Daniel-Vedele F., Gojon A.
    Plant Physiol. 127:262-271(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Nitrate transport in plants: which gene and which control?"
    Orsel M., Filleur S., Fraisier V., Daniel-Vedele F.
    J. Exp. Bot. 53:825-833(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  11. "Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana: responses to nitrate provision."
    Okamoto M., Vidmar J.J., Glass A.D.
    Plant Cell Physiol. 44:304-317(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY NITRATE, GENE FAMILY.
  12. "Disruption of the nitrate transporter genes AtNRT2.1 and AtNRT2.2 restricts growth at low external nitrate concentration."
    Orsel M., Eulenburg K., Krapp A., Daniel-Vedele F.
    Planta 219:714-721(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY NITRATE.
  13. "The putative high-affinity nitrate transporter NRT2.1 represses lateral root initiation in response to nutritional cues."
    Little D.Y., Rao H., Oliva S., Daniel-Vedele F., Krapp A., Malamy J.E.
    Proc. Natl. Acad. Sci. U.S.A. 102:13693-13698(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLY-119, DISRUPTION PHENOTYPE.
  14. "A central role for the nitrate transporter NRT2.1 in the integrated morphological and physiological responses of the root system to nitrogen limitation in Arabidopsis."
    Remans T., Nacry P., Pervent M., Girin T., Tillard P., Lepetit M., Gojon A.
    Plant Physiol. 140:909-921(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  15. "High-affinity nitrate transport in roots of Arabidopsis depends on expression of the NAR2-like gene AtNRT3.1."
    Okamoto M., Kumar A., Li W., Wang Y., Siddiqi M.Y., Crawford N.M., Glass A.D.
    Plant Physiol. 140:1036-1046(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY NITRATE.
  16. "Characterization of a two-component high-affinity nitrate uptake system in Arabidopsis. Physiology and protein-protein interaction."
    Orsel M., Chopin F., Leleu O., Smith S.J., Krapp A., Daniel-Vedele F., Miller A.J.
    Plant Physiol. 142:1304-1317(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NRT3.1, INDUCTION.
  17. "Nitrate transporters and peptide transporters."
    Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.
    FEBS Lett. 581:2290-2300(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  18. "Regulation of root nitrate uptake at the NRT2.1 protein level in Arabidopsis thaliana."
    Wirth J., Chopin F., Santoni V., Viennois G., Tillard P., Krapp A., Lejay L., Daniel-Vedele F., Gojon A.
    J. Biol. Chem. 282:23541-23552(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY LIGHT AND SUCROSE.
  19. "The Arabidopsis ATNRT2.7 nitrate transporter controls nitrate content in seeds."
    Chopin F., Orsel M., Dorbe M.F., Chardon F., Truong H.N., Miller A.J., Krapp A., Daniel-Vedele F.
    Plant Cell 19:1590-1602(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  20. "Identification of a 150 bp cis-acting element of the AtNRT2.1 promoter involved in the regulation of gene expression by the N and C status of the plant."
    Girin T., Lejay L., Wirth J., Widiez T., Palenchar P.M., Nazoa P., Touraine B., Gojon A., Lepetit M.
    Plant Cell Environ. 30:1366-1380(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY NITRATE AND SUCROSE.
  21. "Dissection of the AtNRT2.1:AtNRT2.2 inducible high-affinity nitrate transporter gene cluster."
    Li W., Wang Y., Okamoto M., Crawford N.M., Siddiqi M.Y., Glass A.D.
    Plant Physiol. 143:425-433(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  22. "The Arabidopsis nitrate transporter AtNRT2.1 is targeted to the root plasma membrane."
    Chopin F., Wirth J., Dorbe M.F., Lejay L., Krapp A., Gojon A., Daniel-Vedele F.
    Plant Physiol. Biochem. 45:630-635(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  23. "Nitrate signaling and the two component high affinity uptake system in Arabidopsis."
    Orsel M., Chopin F., Leleu O., Smith S.J., Krapp A., Daniel-Vedele F., Miller A.J.
    Plant Signal. Behav. 2:260-262(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  24. "Characterization of an intact two-component high-affinity nitrate transporter from Arabidopsis roots."
    Yong Z., Kotur Z., Glass A.D.
    Plant J. 63:739-748(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH NRT3.1.

Entry informationi

Entry nameiNRT21_ARATH
AccessioniPrimary (citable) accession number: O82811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: November 1, 1998
Last modified: February 17, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.