Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

High-affinity nitrate transporter 2.1

Gene

NRT2.1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in nitrate transport, but does not seem to be able to mediate transport by its own. Acts as a dual component transporter with NTR3.1. Acts as a repressor of lateral root initiation under high sucrose/low nitrate conditions.6 Publications

GO - Molecular functioni

  • nitrate transmembrane transporter activity Source: TAIR

GO - Biological processi

  • cellular response to nitrate Source: GO_Central
  • lateral root development Source: TAIR
  • nitrate assimilation Source: UniProtKB-KW
  • nitrate transport Source: CACAO
  • response to nitrate Source: TAIR

Keywordsi

Biological processNitrate assimilation

Protein family/group databases

TCDBi2.A.1.8.12 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity nitrate transporter 2.1
Short name:
AtNRT2:1
Alternative name(s):
Protein ACH1
Protein LATERAL ROOT INITIATION 1
Gene namesi
Name:NRT2.1
Synonyms:ACH1, LIN1, NRT2;1AT
Ordered Locus Names:At1g08090
ORF Names:T6D22.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G08090
TAIRilocus:2205190 AT1G08090

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei69 – 89HelicalSequence analysisAdd BLAST21
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Transmembranei129 – 149HelicalSequence analysisAdd BLAST21
Transmembranei151 – 171HelicalSequence analysisAdd BLAST21
Transmembranei193 – 213HelicalSequence analysisAdd BLAST21
Transmembranei226 – 246HelicalSequence analysisAdd BLAST21
Transmembranei271 – 290HelicalSequence analysisAdd BLAST20
Transmembranei310 – 330HelicalSequence analysisAdd BLAST21
Transmembranei352 – 372HelicalSequence analysisAdd BLAST21
Transmembranei383 – 403HelicalSequence analysisAdd BLAST21
Transmembranei410 – 430HelicalSequence analysisAdd BLAST21
Transmembranei441 – 461HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Impaired in the high-affinity nitrate transport. Reduced growth on low-nitrate media. Reduced nitrate uptake and increased lateral root initiation.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi119G → R in lin1; reduced nitrate uptake and increased lateral root initiation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004000981 – 530High-affinity nitrate transporter 2.1Add BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei521PhosphothreonineBy similarity1

Post-translational modificationi

Might be subject to partial proteolysis at the C-terminus.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO82811

PTM databases

iPTMnetiO82811

Expressioni

Tissue specificityi

Root cortical and epidermal cells. Not expressed in new lateral root Primordia. Detected in shoots.4 Publications

Inductioni

Up-regulated at transcript level by light and sucrose, and transiently by nitrate, but no changes at protein level. Down-regulated by ammonium, amino acids and N-metabolites resulting from nitrate reduction. Induced by sudden N starvation and by growth on low nitrate concentration. Circadian-regulation. Expression increases during the light phase and decreases during the dark phase.8 Publications

Gene expression databases

ExpressionAtlasiO82811 baseline and differential
GenevisibleiO82811 AT

Interactioni

Subunit structurei

Monomer. Heterotetramer composed of two NRT2.1 and two NRT3.1. Interacts with NRT3.1.2 Publications

Protein-protein interaction databases

BioGridi22568, 1 interactor
IntActiO82811, 1 interactor
STRINGi3702.AT1G08090.1

Structurei

3D structure databases

ProteinModelPortaliO82811
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IE1F Eukaryota
COG2223 LUCA
HOGENOMiHOG000196276
InParanoidiO82811
KOiK02575
OMAiSEWSHEE
OrthoDBiEOG093607XF
PhylomeDBiO82811

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit

Sequencei

Sequence statusi: Complete.

O82811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDSTGEPGS SMHGVTGREQ SFAFSVQSPI VHTDKTAKFD LPVDTEHKAT
60 70 80 90 100
VFKLFSFAKP HMRTFHLSWI SFSTCFVSTF AAAPLVPIIR ENLNLTKQDI
110 120 130 140 150
GNAGVASVSG SIFSRLVMGA VCDLLGPRYG CAFLVMLSAP TVFSMSFVSD
160 170 180 190 200
AAGFITVRFM IGFCLATFVS CQYWMSTMFN SQIIGLVNGT AAGWGNMGGG
210 220 230 240 250
ITQLLMPIVY EIIRRCGSTA FTAWRIAFFV PGWLHIIMGI LVLNLGQDLP
260 270 280 290 300
DGNRATLEKA GEVAKDKFGK ILWYAVTNYR TWIFVLLYGY SMGVELSTDN
310 320 330 340 350
VIAEYFFDRF HLKLHTAGLI AACFGMANFF ARPAGGYASD FAAKYFGMRG
360 370 380 390 400
RLWTLWIIQT AGGLFCVWLG RANTLVTAVV AMVLFSMGAQ AACGATFAIV
410 420 430 440 450
PFVSRRALGI ISGLTGAGGN FGSGLTQLLF FSTSHFTTEQ GLTWMGVMIV
460 470 480 490 500
ACTLPVTLVH FPQWGSMFLP PSTDPVKGTE AHYYGSEWNE QEKQKNMHQG
510 520 530
SLRFAENAKS EGGRRVRSAA TPPENTPNNV
Length:530
Mass (Da):57,710
Last modified:November 1, 1998 - v1
Checksum:iA40F435039635090
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97058 mRNA Translation: CAB09794.1
AF019748 mRNA Translation: AAC35883.1
AF093754 mRNA Translation: AAC64170.1
AC026875 Genomic DNA Translation: AAF79826.1
CP002684 Genomic DNA Translation: AEE28241.1
PIRiT51836
RefSeqiNP_172288.1, NM_100684.3
UniGeneiAt.5407

Genome annotation databases

EnsemblPlantsiAT1G08090.1; AT1G08090.1; AT1G08090
GeneIDi837327
GrameneiAT1G08090.1; AT1G08090.1; AT1G08090
KEGGiath:AT1G08090

Similar proteinsi

Entry informationi

Entry nameiNRT21_ARATH
AccessioniPrimary (citable) accession number: O82811
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health