ID   SRPP_HEVBR              Reviewed;         204 AA.
AC   O82803; Q9S8I3; Q9S8I4; Q9S8I5; Q9S9G7;
DT   20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   27-MAR-2024, entry version 89.
DE   RecName: Full=Small rubber particle protein {ECO:0000303|PubMed:10358068};
DE            Short=HbSRPP {ECO:0000303|PubMed:10358068};
DE   AltName: Full=22 kDa rubber particle protein;
DE            Short=22 kDa RPP;
DE   AltName: Full=27 kDa natural rubber allergen;
DE   AltName: Full=Latex allergen Hev b 3 {ECO:0000303|PubMed:8828541};
DE   AltName: Allergen=Hev b 3 {ECO:0000303|PubMed:8828541};
GN   Name=SRPP {ECO:0000303|PubMed:10358068};
GN   Synonyms=HEVB3 {ECO:0000303|PubMed:8828541};
OS   Hevea brasiliensis (Para rubber tree) (Siphonia brasiliensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Micrandreae;
OC   Hevea.
OX   NCBI_TaxID=3981;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND FUNCTION.
RC   STRAIN=cv. RRIM 600; TISSUE=Latex;
RX   PubMed=10358068; DOI=10.1074/jbc.274.24.17132;
RA   Oh S.K., Kang H., Shin D.H., Yang J., Chow K.-S., Yeang H.Y., Wagner B.,
RA   Breiteneder H., Han K.-H.;
RT   "Isolation, characterization, and functional analysis of a novel cDNA clone
RT   encoding a small rubber particle protein from Hevea brasiliensis.";
RL   J. Biol. Chem. 274:17132-17138(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. RRIM 600; TISSUE=Latex;
RX   PubMed=10550757; DOI=10.1016/s0091-6749(99)70093-x;
RA   Wagner B., Krebitz M., Buck D., Niggemann B., Yeang H.Y., Han K.-H.,
RA   Scheiner O., Breiteneder H.;
RT   "Cloning, expression, and characterization of recombinant Hev b 3, a Hevea
RT   brasiliensis protein associated with latex allergy in patients with spina
RT   bifida.";
RL   J. Allergy Clin. Immunol. 104:1084-1092(1999).
RN   [3]
RP   PROTEIN SEQUENCE OF 12-17; 23-31; 36-50; 54-68; 73-86; 91-104; 106-123;
RP   125-143 AND 175-192.
RC   TISSUE=Latex;
RX   PubMed=7888787; DOI=10.1159/000236851;
RA   Alenius H., Kalkkinen N., Lukka M., Turjanmaa K., Reunala T.,
RA   Maekinen-Kiljunen S., Palosuo T.;
RT   "Purification and partial amino acid sequencing of a 27-kD natural rubber
RT   allergen recognized by latex-allergic children with spina bifida.";
RL   Int. Arch. Allergy Immunol. 106:258-262(1995).
RN   [4]
RP   PROTEIN SEQUENCE OF 8-33; 36-49; 54-68; 73-90 AND 178-194.
RC   TISSUE=Latex;
RX   PubMed=7650400;
RA   Lu L.-J., Kurup V.P., Hoffman D.R., Kelly K.J., Murali P.S., Fink J.N.;
RT   "Characterization of a major latex allergen associated with
RT   hypersensitivity in spina bifida patients.";
RL   J. Immunol. 155:2721-2728(1995).
RN   [5]
RP   PROTEIN SEQUENCE OF 18-35; 54-68; 73-90; 106-122 AND 183-194, AND MASS
RP   SPECTROMETRY.
RC   STRAIN=cv. Costa Rica, and cv. RRIM 600; TISSUE=Latex;
RX   PubMed=9636811; DOI=10.1111/j.1398-9995.1998.tb04089.x;
RA   Yeang H.Y., Ward M.A., Zamri A.S.M., Dennis M.S., Light D.R.;
RT   "Amino acid sequence similarity of Hev b 3 to two previously reported
RT   27- and 23-kDa latex proteins allergenic to spina bifida patients.";
RL   Allergy 53:513-519(1998).
RN   [6]
RP   ALLERGEN.
RX   PubMed=8828541; DOI=10.1016/s0091-6749(96)70097-0;
RA   Yeang H.Y., Cheong K.F., Sunderasan E., Hamzah S., Chew N.P., Hamid S.,
RA   Hamilton R.G., Cardosa M.J.;
RT   "The 14.6 kd rubber elongation factor (Hev b 1) and 24 kd (Hev b 3) rubber
RT   particle proteins are recognized by IgE from patients with spina bifida and
RT   latex allergy.";
RL   J. Allergy Clin. Immunol. 98:628-639(1996).
RN   [7]
RP   EPITOPE MAPPING.
RX   PubMed=11275264; DOI=10.1016/s0161-5890(00)00095-x;
RA   Banerjee B., Kanitpong K., Fink J.N., Zussman M., Sussman G.L., Kelly K.J.,
RA   Kurup V.P.;
RT   "Unique and shared IgE epitopes of Hev b 1 and Hev b 3 in latex allergy.";
RL   Mol. Immunol. 37:789-798(2000).
RN   [8]
RP   INDUCTION.
RX   PubMed=12461132; DOI=10.1093/pcp/pcf161;
RA   Sookmark U., Pujade-Renaud V., Chrestin H., Lacote R., Naiyanetr C.,
RA   Seguin M., Romruensukharom P., Narangajavana J.;
RT   "Characterization of polypeptides accumulated in the latex cytosol of
RT   rubber trees affected by the tapping panel dryness syndrome.";
RL   Plant Cell Physiol. 43:1323-1333(2002).
RN   [9]
RP   TISSUE SPECIFICITY.
RX   PubMed=19415323; DOI=10.1007/s00425-009-0936-0;
RA   Sando T., Hayashi T., Takeda T., Akiyama Y., Nakazawa Y., Fukusaki E.,
RA   Kobayashi A.;
RT   "Histochemical study of detailed laticifer structure and rubber
RT   biosynthesis-related protein localization in Hevea brasiliensis using
RT   spectral confocal laser scanning microscopy.";
RL   Planta 230:215-225(2009).
RN   [10]
RP   INDUCTION BY ETHYLENE.
RX   PubMed=22162870; DOI=10.1093/jxb/err363;
RA   Chow K.S., Mat-Isa M.N., Bahari A., Ghazali A.K., Alias H.,
RA   Mohd-Zainuddin Z., Hoh C.C., Wan K.L.;
RT   "Metabolic routes affecting rubber biosynthesis in Hevea brasiliensis
RT   latex.";
RL   J. Exp. Bot. 63:1863-1871(2012).
RN   [11]
RP   SUBCELLULAR LOCATION.
RX   PubMed=24036080; DOI=10.1016/j.bbamem.2013.08.025;
RA   Berthelot K., Lecomte S., Estevez Y., Zhendre V., Henry S., Thevenot J.,
RA   Dufourc E.J., Alves I.D., Peruch F.;
RT   "Rubber particle proteins, HbREF and HbSRPP, show different interactions
RT   with model membranes.";
RL   Biochim. Biophys. Acta 1838:287-299(2014).
RN   [12]
RP   SUBUNIT, AND LACK OF GLYCOSYLATION.
RX   PubMed=24239687; DOI=10.1016/j.bbapap.2013.10.017;
RA   Berthelot K., Lecomte S., Estevez Y., Coulary-Salin B., Peruch F.;
RT   "Homologous Hevea brasiliensis REF (Hevb1) and SRPP (Hevb3) present
RT   different auto-assembling.";
RL   Biochim. Biophys. Acta 1844:473-485(2014).
RN   [13]
RP   REVIEW.
RX   PubMed=25019490; DOI=10.1016/j.biochi.2014.07.002;
RA   Berthelot K., Lecomte S., Estevez Y., Peruch F.;
RT   "Hevea brasiliensis REF (Hev b 1) and SRPP (Hev b 3): An overview on rubber
RT   particle proteins.";
RL   Biochimie 106:1-9(2014).
CC   -!- FUNCTION: Involved in the biosynthesis of rubber, an isoprenoid polymer
CC       (cis-1,4-polyisoprene). {ECO:0000269|PubMed:10358068}.
CC   -!- SUBUNIT: Auto-assembles in solution into stable nanomultimers of a
CC       globular nature. {ECO:0000269|PubMed:24239687}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24036080}.
CC       Note=Found in latex, the cytoplasm of laticifer cells. Tightly bound to
CC       small rubber particles, where it displays a covering effect on the
CC       lipid headgroups of the membrane without disturbing its integrity.
CC       {ECO:0000269|PubMed:24036080}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in the specialized vessel
CC       laticifers, but localized only in the laticifer layers in the
CC       conducting phloem (PubMed:10358068, PubMed:19415323). Also detected in
CC       leaves (PubMed:10358068). {ECO:0000269|PubMed:10358068,
CC       ECO:0000269|PubMed:19415323}.
CC   -!- INDUCTION: Induced by tapping (PubMed:12461132). Not induced by
CC       wounding, ethephon or abscisic acid treatment (PubMed:12461132).
CC       Induced by ethylene (PubMed:22162870). {ECO:0000269|PubMed:12461132,
CC       ECO:0000269|PubMed:22162870}.
CC   -!- PTM: Not glycosylated. {ECO:0000269|PubMed:24239687}.
CC   -!- PTM: The N-terminus is blocked. {ECO:0000269|PubMed:7888787}.
CC   -!- PTM: Consistent shifts of about 266 Da observed by MS in various forms
CC       of the intact protein suggest the addition of stearolyl groups.
CC       {ECO:0000305|PubMed:9636811}.
CC   -!- MASS SPECTROMETRY: Mass=22258; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:9636811};
CC   -!- MASS SPECTROMETRY: Mass=22524; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:9636811};
CC   -!- MASS SPECTROMETRY: Mass=22791; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:9636811};
CC   -!- MASS SPECTROMETRY: Mass=23058; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:9636811};
CC   -!- ALLERGEN: Causes an allergic reaction in human. Involved in latex
CC       allergic reactions. Major spina bifida (SB) associated latex allergen.
CC       Patients with SB are especially sensitive to specific allergens that do
CC       not usually affect adult patients without SB who are otherwise
CC       sensitive to latex. These patients are multioperated patients, whose
CC       mucosal membranes have been frequently in contact with latex products.
CC       Many patients with SB are sensitized to low molecular weight
CC       polypeptides derived from the fragmentation of Hev b 3.
CC       {ECO:0000269|PubMed:8828541}.
CC   -!- SIMILARITY: Belongs to the REF/SRPP family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=Gloves, condoms and bouncy
CC       balls - Issue 13 of August 2001;
CC       URL="https://web.expasy.org/spotlight/back_issues/013";
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DR   EMBL; AF051317; AAC82355.1; -; mRNA.
DR   EMBL; AJ223388; CAA11303.1; -; mRNA.
DR   EMBL; AJ223389; CAA11304.1; -; mRNA.
DR   EMBL; AJ223390; CAA11305.1; -; mRNA.
DR   PIR; T10766; T10766.
DR   AlphaFoldDB; O82803; -.
DR   Allergome; 3314; Hev b 3.0101.
DR   Allergome; 387; Hev b 3.
DR   OrthoDB; 1222185at2759; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR008802; REF.
DR   PANTHER; PTHR33732; REF/SRPP-LIKE PROTEIN OS05G0151300/LOC_OS05G05940; 1.
DR   PANTHER; PTHR33732:SF9; REF_SRPP-LIKE PROTEIN OS05G0151300_LOC_OS05G05940; 1.
DR   Pfam; PF05755; REF; 1.
PE   1: Evidence at protein level;
KW   Allergen; Cytoplasm; Direct protein sequencing.
FT   CHAIN           1..204
FT                   /note="Small rubber particle protein"
FT                   /id="PRO_0000221061"
FT   CONFLICT        39
FT                   /note="G -> Y (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        44
FT                   /note="G -> P (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        49
FT                   /note="E -> K (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        78
FT                   /note="V -> P (in Ref. 5; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        78
FT                   /note="V -> T (in Ref. 5; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        118..122
FT                   /note="GVQEG -> RKEKK (in Ref. 5; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        192
FT                   /note="T -> G (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   204 AA;  22345 MW;  2A3BAE7D8325D833 CRC64;
     MAEEVEEERL KYLDFVRAAG VYAVDSFSTL YLYAKDISGP LKPGVDTIEN VVKTVVTPVY
     YIPLEAVKFV DKTVDVSVTS LDGVVPPVIK QVSAQTYSVA QDAPRIVLDV ASSVFNTGVQ
     EGAKALYANL EPKAEQYAVI TWRALNKLPL VPQVANVVVP TAVYFSEKYN DVVRGTTEQG
     YRVSSYLPLL PTEKITKVFG DEAS
//