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O82803

- SRPP_HEVBR

UniProt

O82803 - SRPP_HEVBR

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Protein

Small rubber particle protein

Gene

SRPP

Organism
Hevea brasiliensis (Para rubber tree) (Siphonia brasiliensis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of rubber, an isoprenoid polymer (cis-1,4-polyisoprene).

Names & Taxonomyi

Protein namesi
Recommended name:
Small rubber particle protein
Short name:
SRPP
Alternative name(s):
22 kDa rubber particle protein
Short name:
22 kDa RPP
27 kDa natural rubber allergen
Latex allergen Hev b 3
Allergen: Hev b 3
Gene namesi
Name:SRPP
Synonyms:HEVB3
OrganismiHevea brasiliensis (Para rubber tree) (Siphonia brasiliensis)
Taxonomic identifieri3981 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeCrotonoideaeMicrandreaeHevea

Subcellular locationi

Cytoplasm
Note: Found in latex, the cytoplasm of laticifer cells. Tightly bound to small rubber particles.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Involved in latex allergic reactions. Major spina bifida (SB) associated latex allergen. Patients with SB are especially sensitive to specific allergens that do not usually affect adult patients without SB who are otherwise sensitive to latex. These patients are multioperated patients, whose mucosal membranes have been frequently in contact with latex products. Many patients with SB are sensitized to low molecular weight polypeptides derived from the fragmentation of Hev b 3.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3314. Hev b 3.0101.
387. Hev b 3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Small rubber particle proteinPRO_0000221061Add
BLAST

Post-translational modificationi

The N-terminus is blocked.
Consistent shifts of about 266 Da observed by MS in various forms of the intact protein suggest the addition of stearolyl groups.

Proteomic databases

PRIDEiO82803.

Expressioni

Tissue specificityi

Highly expressed in the specialized vessel laticifers. Also detected in leaves.

Inductioni

Not induced by wounding or ethephon treatment.

Family & Domainsi

Sequence similaritiesi

Belongs to the REF/SRPP family.Curated

Family and domain databases

InterProiIPR008802. REF.
[Graphical view]
PfamiPF05755. REF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O82803-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEEVEEERL KYLDFVRAAG VYAVDSFSTL YLYAKDISGP LKPGVDTIEN
60 70 80 90 100
VVKTVVTPVY YIPLEAVKFV DKTVDVSVTS LDGVVPPVIK QVSAQTYSVA
110 120 130 140 150
QDAPRIVLDV ASSVFNTGVQ EGAKALYANL EPKAEQYAVI TWRALNKLPL
160 170 180 190 200
VPQVANVVVP TAVYFSEKYN DVVRGTTEQG YRVSSYLPLL PTEKITKVFG

DEAS
Length:204
Mass (Da):22,345
Last modified:November 1, 1998 - v1
Checksum:i2A3BAE7D8325D833
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti39 – 391G → Y AA sequence (PubMed:7650400)Curated
Sequence conflicti44 – 441G → P AA sequence (PubMed:7650400)Curated
Sequence conflicti49 – 491E → K AA sequence (PubMed:7650400)Curated
Sequence conflicti78 – 781V → P AA sequence (PubMed:9636811)Curated
Sequence conflicti78 – 781V → T AA sequence (PubMed:9636811)Curated
Sequence conflicti118 – 1225GVQEG → RKEKK AA sequence (PubMed:9636811)Curated
Sequence conflicti192 – 1921T → G AA sequence (PubMed:7888787)Curated

Mass spectrometryi

Molecular mass is 22258 Da from positions 1 - 204. Determined by ESI. 1 Publication
Molecular mass is 22524 Da from positions 1 - 204. Determined by ESI. 1 Publication
Molecular mass is 22791 Da from positions 1 - 204. Determined by ESI. 1 Publication
Molecular mass is 23058 Da from positions 1 - 204. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051317 mRNA. Translation: AAC82355.1.
AJ223388 mRNA. Translation: CAA11303.1.
AJ223389 mRNA. Translation: CAA11304.1.
AJ223390 mRNA. Translation: CAA11305.1.
PIRiT10766.

Cross-referencesi

Web resourcesi

Protein Spotlight

Gloves, condoms and bouncy balls - Issue 13 of August 2001

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051317 mRNA. Translation: AAC82355.1 .
AJ223388 mRNA. Translation: CAA11303.1 .
AJ223389 mRNA. Translation: CAA11304.1 .
AJ223390 mRNA. Translation: CAA11305.1 .
PIRi T10766.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 3314. Hev b 3.0101.
387. Hev b 3.

Proteomic databases

PRIDEi O82803.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008802. REF.
[Graphical view ]
Pfami PF05755. REF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation, characterization, and functional analysis of a novel cDNA clone encoding a small rubber particle protein from Hevea brasiliensis."
    Oh S.K., Kang H., Shin D.H., Yang J., Chow K.-S., Yeang H.Y., Wagner B., Breiteneder H., Han K.-H.
    J. Biol. Chem. 274:17132-17138(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. RRIM 600.
    Tissue: Latex.
  2. "Cloning, expression, and characterization of recombinant Hev b 3, a Hevea brasiliensis protein associated with latex allergy in patients with spina bifida."
    Wagner B., Krebitz M., Buck D., Niggemann B., Yeang H.Y., Han K.-H., Scheiner O., Breiteneder H.
    J. Allergy Clin. Immunol. 104:1084-1092(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. RRIM 600.
    Tissue: Latex.
  3. "Purification and partial amino acid sequencing of a 27-kD natural rubber allergen recognized by latex-allergic children with spina bifida."
    Alenius H., Kalkkinen N., Lukka M., Turjanmaa K., Reunala T., Maekinen-Kiljunen S., Palosuo T.
    Int. Arch. Allergy Immunol. 106:258-262(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 12-17; 23-31; 36-50; 54-68; 73-86; 91-104; 106-123; 125-143 AND 175-192.
    Tissue: Latex.
  4. "Characterization of a major latex allergen associated with hypersensitivity in spina bifida patients."
    Lu L.-J., Kurup V.P., Hoffman D.R., Kelly K.J., Murali P.S., Fink J.N.
    J. Immunol. 155:2721-2728(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 8-33; 36-49; 54-68; 73-90 AND 178-194.
    Tissue: Latex.
  5. "Amino acid sequence similarity of Hev b 3 to two previously reported 27- and 23-kDa latex proteins allergenic to spina bifida patients."
    Yeang H.Y., Ward M.A., Zamri A.S.M., Dennis M.S., Light D.R.
    Allergy 53:513-519(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 18-35; 54-68; 73-90; 106-122 AND 183-194, MASS SPECTROMETRY.
    Strain: cv. Costa Rica and cv. RRIM 600.
    Tissue: Latex.
  6. "Unique and shared IgE epitopes of Hev b 1 and Hev b 3 in latex allergy."
    Banerjee B., Kanitpong K., Fink J.N., Zussman M., Sussman G.L., Kelly K.J., Kurup V.P.
    Mol. Immunol. 37:789-798(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: EPITOPE MAPPING.

Entry informationi

Entry nameiSRPP_HEVBR
AccessioniPrimary (citable) accession number: O82803
Secondary accession number(s): Q9S8I3
, Q9S8I4, Q9S8I5, Q9S9G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3