O82803 (SRPP_HEVBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Small rubber particle protein
Short name=SRPP

Alternative name(s):
22 kDa rubber particle protein
Short name=22 kDa RPP
27 kDa natural rubber allergen
Latex allergen Hev b 3
Allergen=Hev b 3

Gene names

Name:	SRPP
Synonyms:	HEVB3

Organism

Hevea brasiliensis (Para rubber tree) (Siphonia brasiliensis)

Taxonomic identifier

3981 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Malpighiales › Euphorbiaceae › Crotonoideae › Micrandreae › Hevea

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the biosynthesis of rubber, an isoprenoid polymer (cis-1,4-polyisoprene).
Subcellular location	Cytoplasm. Note: Found in latex, the cytoplasm of laticifer cells. Tightly bound to small rubber particles.
Tissue specificity	Highly expressed in the specialized vessel laticifers. Also detected in leaves.
Induction	Not induced by wounding or ethephon treatment.
Post-translational modification	The N-terminus is blocked. Consistent shifts of about 266 Da observed by MS in various forms of the intact protein suggest the addition of stearolyl groups.
Allergenic properties	Causes an allergic reaction in human. Involved in latex allergic reactions. Major spina bifida (SB) associated latex allergen. Patients with SB are especially sensitive to specific allergens that do not usually affect adult patients without SB who are otherwise sensitive to latex. These patients are multioperated patients, whose mucosal membranes have been frequently in contact with latex products. Many patients with SB are sensitized to low molecular weight polypeptides derived from the fragmentation of Hev b 3.
Sequence similarities	Belongs to the REF/SRPP family.
Mass spectrometry	Molecular mass is 22258 Da from positions 1 - 204. Determined by ESI. Ref.5 Molecular mass is 22524 Da from positions 1 - 204. Determined by ESI. Ref.5 Molecular mass is 22791 Da from positions 1 - 204. Determined by ESI. Ref.5 Molecular mass is 23058 Da from positions 1 - 204. Determined by ESI. Ref.5

Ontologies

Keywords
Cellular component	Cytoplasm
Disease	Allergen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	Small rubber particle protein		PRO_0000221061
Experimental info
	Sequence conflict	39	1	G → Y AA sequence Ref.4
	Sequence conflict	44	1	G → P AA sequence Ref.4
	Sequence conflict	49	1	E → K AA sequence Ref.4
	Sequence conflict	78	1	V → P AA sequence Ref.5
	Sequence conflict	78	1	V → T AA sequence Ref.5
	Sequence conflict	118 – 122	5	GVQEG → RKEKK AA sequence Ref.5
	Sequence conflict	192	1	T → G AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O82803 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 2A3BAE7D8325D833	FASTA	204	22,345
	10 20 30 40 50 60 MAEEVEEERL KYLDFVRAAG VYAVDSFSTL YLYAKDISGP LKPGVDTIEN VVKTVVTPVY 70 80 90 100 110 120 YIPLEAVKFV DKTVDVSVTS LDGVVPPVIK QVSAQTYSVA QDAPRIVLDV ASSVFNTGVQ 130 140 150 160 170 180 EGAKALYANL EPKAEQYAVI TWRALNKLPL VPQVANVVVP TAVYFSEKYN DVVRGTTEQG 190 200 YRVSSYLPLL PTEKITKVFG DEAS

References

[1]	"Isolation, characterization, and functional analysis of a novel cDNA clone encoding a small rubber particle protein from Hevea brasiliensis." Oh S.K., Kang H., Shin D.H., Yang J., Chow K.-S., Yeang H.Y., Wagner B., Breiteneder H., Han K.-H. J. Biol. Chem. 274:17132-17138(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. RRIM 600. Tissue: Latex.
[2]	"Cloning, expression, and characterization of recombinant Hev b 3, a Hevea brasiliensis protein associated with latex allergy in patients with spina bifida." Wagner B., Krebitz M., Buck D., Niggemann B., Yeang H.Y., Han K.-H., Scheiner O., Breiteneder H. J. Allergy Clin. Immunol. 104:1084-1092(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. RRIM 600. Tissue: Latex.
[3]	"Purification and partial amino acid sequencing of a 27-kD natural rubber allergen recognized by latex-allergic children with spina bifida." Alenius H., Kalkkinen N., Lukka M., Turjanmaa K., Reunala T., Maekinen-Kiljunen S., Palosuo T. Int. Arch. Allergy Immunol. 106:258-262(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 12-17; 23-31; 36-50; 54-68; 73-86; 91-104; 106-123; 125-143 AND 175-192. Tissue: Latex.
[4]	"Characterization of a major latex allergen associated with hypersensitivity in spina bifida patients." Lu L.-J., Kurup V.P., Hoffman D.R., Kelly K.J., Murali P.S., Fink J.N. J. Immunol. 155:2721-2728(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 8-33; 36-49; 54-68; 73-90 AND 178-194. Tissue: Latex.
[5]	"Amino acid sequence similarity of Hev b 3 to two previously reported 27- and 23-kDa latex proteins allergenic to spina bifida patients." Yeang H.Y., Ward M.A., Zamri A.S.M., Dennis M.S., Light D.R. Allergy 53:513-519(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 18-35; 54-68; 73-90; 106-122 AND 183-194, MASS SPECTROMETRY. Strain: cv. Costa Rica and cv. RRIM 600. Tissue: Latex.
[6]	"Unique and shared IgE epitopes of Hev b 1 and Hev b 3 in latex allergy." Banerjee B., Kanitpong K., Fink J.N., Zussman M., Sussman G.L., Kelly K.J., Kurup V.P. Mol. Immunol. 37:789-798(2000) [PubMed] [Europe PMC] [Abstract] Cited for: EPITOPE MAPPING.

Web resources

Protein Spotlight

Gloves, condoms and bouncy balls - Issue 13 of August 2001

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF051317 mRNA. Translation: AAC82355.1. AJ223388 mRNA. Translation: CAA11303.1. AJ223389 mRNA. Translation: CAA11304.1. AJ223390 mRNA. Translation: CAA11305.1.
PIR	T10766.
3D structure databases
ModBase	Search...
Protein family/group databases
Allergome	3314. Hev b 3.0101. 387. Hev b 3.
Proteomic databases
PRIDE	O82803.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008802. REF. [Graphical view]
Pfam	PF05755. REF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SRPP_HEVBR

Accession

Primary (citable) accession number: O82803
Secondary accession number(s): Q9S8I3 , Q9S8I4, Q9S8I5, Q9S9G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program