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O82803 (SRPP_HEVBR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Small rubber particle protein

Short name=SRPP
Alternative name(s):
22 kDa rubber particle protein
Short name=22 kDa RPP
27 kDa natural rubber allergen
Latex allergen Hev b 3
Allergen=Hev b 3
Gene names
Name:SRPP
Synonyms:HEVB3
OrganismHevea brasiliensis (Para rubber tree) (Siphonia brasiliensis)
Taxonomic identifier3981 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeCrotonoideaeMicrandreaeHevea

Protein attributes

Sequence length204 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the biosynthesis of rubber, an isoprenoid polymer (cis-1,4-polyisoprene).

Subcellular location

Cytoplasm. Note: Found in latex, the cytoplasm of laticifer cells. Tightly bound to small rubber particles.

Tissue specificity

Highly expressed in the specialized vessel laticifers. Also detected in leaves.

Induction

Not induced by wounding or ethephon treatment.

Post-translational modification

The N-terminus is blocked.

Consistent shifts of about 266 Da observed by MS in various forms of the intact protein suggest the addition of stearolyl groups.

Allergenic properties

Causes an allergic reaction in human. Involved in latex allergic reactions. Major spina bifida (SB) associated latex allergen. Patients with SB are especially sensitive to specific allergens that do not usually affect adult patients without SB who are otherwise sensitive to latex. These patients are multioperated patients, whose mucosal membranes have been frequently in contact with latex products. Many patients with SB are sensitized to low molecular weight polypeptides derived from the fragmentation of Hev b 3.

Sequence similarities

Belongs to the REF/SRPP family.

Mass spectrometry

Molecular mass is 22258 Da from positions 1 - 204. Determined by ESI. Ref.5

Molecular mass is 22524 Da from positions 1 - 204. Determined by ESI. Ref.5

Molecular mass is 22791 Da from positions 1 - 204. Determined by ESI. Ref.5

Molecular mass is 23058 Da from positions 1 - 204. Determined by ESI. Ref.5

Ontologies

Keywords
   Cellular componentCytoplasm
   DiseaseAllergen
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 204204Small rubber particle protein
PRO_0000221061

Experimental info

Sequence conflict391G → Y AA sequence Ref.4
Sequence conflict441G → P AA sequence Ref.4
Sequence conflict491E → K AA sequence Ref.4
Sequence conflict781V → P AA sequence Ref.5
Sequence conflict781V → T AA sequence Ref.5
Sequence conflict118 – 1225GVQEG → RKEKK AA sequence Ref.5
Sequence conflict1921T → G AA sequence Ref.3

Sequences

Sequence LengthMass (Da)Tools
O82803 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 2A3BAE7D8325D833

FASTA20422,345
        10         20         30         40         50         60 
MAEEVEEERL KYLDFVRAAG VYAVDSFSTL YLYAKDISGP LKPGVDTIEN VVKTVVTPVY 

        70         80         90        100        110        120 
YIPLEAVKFV DKTVDVSVTS LDGVVPPVIK QVSAQTYSVA QDAPRIVLDV ASSVFNTGVQ 

       130        140        150        160        170        180 
EGAKALYANL EPKAEQYAVI TWRALNKLPL VPQVANVVVP TAVYFSEKYN DVVRGTTEQG 

       190        200 
YRVSSYLPLL PTEKITKVFG DEAS 

« Hide

References

[1]"Isolation, characterization, and functional analysis of a novel cDNA clone encoding a small rubber particle protein from Hevea brasiliensis."
Oh S.K., Kang H., Shin D.H., Yang J., Chow K.-S., Yeang H.Y., Wagner B., Breiteneder H., Han K.-H.
J. Biol. Chem. 274:17132-17138(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. RRIM 600.
Tissue: Latex.
[2]"Cloning, expression, and characterization of recombinant Hev b 3, a Hevea brasiliensis protein associated with latex allergy in patients with spina bifida."
Wagner B., Krebitz M., Buck D., Niggemann B., Yeang H.Y., Han K.-H., Scheiner O., Breiteneder H.
J. Allergy Clin. Immunol. 104:1084-1092(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. RRIM 600.
Tissue: Latex.
[3]"Purification and partial amino acid sequencing of a 27-kD natural rubber allergen recognized by latex-allergic children with spina bifida."
Alenius H., Kalkkinen N., Lukka M., Turjanmaa K., Reunala T., Maekinen-Kiljunen S., Palosuo T.
Int. Arch. Allergy Immunol. 106:258-262(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 12-17; 23-31; 36-50; 54-68; 73-86; 91-104; 106-123; 125-143 AND 175-192.
Tissue: Latex.
[4]"Characterization of a major latex allergen associated with hypersensitivity in spina bifida patients."
Lu L.-J., Kurup V.P., Hoffman D.R., Kelly K.J., Murali P.S., Fink J.N.
J. Immunol. 155:2721-2728(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 8-33; 36-49; 54-68; 73-90 AND 178-194.
Tissue: Latex.
[5]"Amino acid sequence similarity of Hev b 3 to two previously reported 27- and 23-kDa latex proteins allergenic to spina bifida patients."
Yeang H.Y., Ward M.A., Zamri A.S.M., Dennis M.S., Light D.R.
Allergy 53:513-519(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 18-35; 54-68; 73-90; 106-122 AND 183-194, MASS SPECTROMETRY.
Strain: cv. Costa Rica and cv. RRIM 600.
Tissue: Latex.
[6]"Unique and shared IgE epitopes of Hev b 1 and Hev b 3 in latex allergy."
Banerjee B., Kanitpong K., Fink J.N., Zussman M., Sussman G.L., Kelly K.J., Kurup V.P.
Mol. Immunol. 37:789-798(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: EPITOPE MAPPING.

Web resources

Protein Spotlight

Gloves, condoms and bouncy balls - Issue 13 of August 2001

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF051317 mRNA. Translation: AAC82355.1.
AJ223388 mRNA. Translation: CAA11303.1.
AJ223389 mRNA. Translation: CAA11304.1.
AJ223390 mRNA. Translation: CAA11305.1.
PIRT10766.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome3314. Hev b 3.0101.
387. Hev b 3.

Proteomic databases

PRIDEO82803.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR008802. REF.
[Graphical view]
PfamPF05755. REF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSRPP_HEVBR
AccessionPrimary (citable) accession number: O82803
Secondary accession number(s): Q9S8I3 expand/collapse secondary AC list , Q9S8I4, Q9S8I5, Q9S9G7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 1, 1998
Last modified: February 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

Allergens

Nomenclature of allergens and list of entries