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Protein

Sulfite reductase 1 [ferredoxin], chloroplastic

Gene

SIR1

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential protein with sulfite reductase activity required in assimilatory sulfate reduction pathway during both primary and secondary metabolism and thus involved in development and growth.By similarity
DNA-binding protein that binds to both double-stranded and single-stranded DNA without significant sequence specificity to reversibly repress the transcriptional activity of chloroplast nucleoids by promoting DNA compaction and possibly regulate DNA replication.By similarity

Catalytic activityi

Hydrogen sulfide + 6 oxidized ferredoxin [iron-sulfur] cluster + 3 H2O = sulfite + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+.By similarity

Cofactori

Protein has several cofactor binding sites:
  • sirohemeBy similarityNote: Binds 1 siroheme per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi502 – 5021Iron-sulfur (4Fe-4S)By similarity
Metal bindingi508 – 5081Iron-sulfur (4Fe-4S)By similarity
Metal bindingi548 – 5481Iron-sulfur (4Fe-4S)By similarity
Metal bindingi552 – 5521Iron (siroheme axial ligand)By similarity
Metal bindingi552 – 5521Iron-sulfur (4Fe-4S)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, DNA-binding, Heme, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase 1 [ferredoxin], chloroplastic (EC:1.8.7.1)
Short name:
NtSiR1
Gene namesi
Name:SIR1
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6262Chloroplast1 PublicationAdd
BLAST
Chaini63 – 693631Sulfite reductase 1 [ferredoxin], chloroplasticPRO_0000416845Add
BLAST

Post-translational modificationi

Phosphorylated; this phosphorylation reduces DNA-binding.By similarity

Keywords - PTMi

Thioether bond

Expressioni

Tissue specificityi

Expressed in leaves, stems, roots and petals.1 Publication

Interactioni

Subunit structurei

Monomer. Interacts with ferredoxin (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO82802.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
InterProiIPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
IPR011787. SiR_ferredoxin-dep.
[Graphical view]
PfamiPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
TIGRFAMsiTIGR02042. sir. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O82802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSFGAAIN IAVADDPNPK LQIHNFSGLK STSNSLLLSR RLHVFQSFSP
60 70 80 90 100
SNPSSIVRAV STPAKPAAVE PKRSKVEIFK EQSNFIRYPL NEEILNDAPN
110 120 130 140 150
INEAATQLIK FHGSYMQYDR DERGGRSYSF MLRTKNPGGE VPNRLYLVMD
160 170 180 190 200
DLADQFGIGT LRLTTRQTFQ LHGVLKKNLK TVMSTIIKNM GSTLGACGDL
210 220 230 240 250
NRNVLAPAAP FAKKDYMFAK QTADNIAALL TPQSGFYYDV WVDGEKVMTA
260 270 280 290 300
EPPEVVKARN DNSHGTNFPD SPEPIYGTQF LPRKFKIAVT VPTDNSVDIF
310 320 330 340 350
TNDIGVVVVS NEDGEPQGFN IYVGGGMGRT HRMETTFPRL AEPLGYVPKE
360 370 380 390 400
DILYAVKAIV VTQRENGRRD DRRYSRLKYL LSSWGIEKFR SVTEQYYGKK
410 420 430 440 450
FQPCRELPEW EFKSYLGWHE AGDGSLFCGL HVDNGRVKGA MKKALREVIE
460 470 480 490 500
KYNLNVRLTP NQNIILCNIR QAWKRPITTV LAQGGLLQPR YVDPLNLTAM
510 520 530 540 550
ACPAFPLCPL AITEAERGIP DILKRVRAIF ERVGLKYSES VVIRITGCPN
560 570 580 590 600
GCARPYMAEL GLVGDGPNSY QIWLGGTPNQ TSLAKTFKDK LKVQDLEKVL
610 620 630 640 650
EPLFFHWRRK RQSKESFGDF TNRMGFEKLG EFVEKWEGIP ESSSRYNLKL
660 670 680 690
FADRETYEAM DALASIQDKN AHQLAIEVVR NYVASQQNGK SMD
Length:693
Mass (Da):77,963
Last modified:November 1, 1998 - v1
Checksum:iF93F365E9AD816C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83583 mRNA. Translation: BAA33531.1.
AB010717 Genomic DNA. Translation: BAA33796.1.
PIRiJE0260.
UniGeneiNta.7003.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83583 mRNA. Translation: BAA33531.1.
AB010717 Genomic DNA. Translation: BAA33796.1.
PIRiJE0260.
UniGeneiNta.7003.

3D structure databases

ProteinModelPortaliO82802.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
InterProiIPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
IPR011787. SiR_ferredoxin-dep.
[Graphical view]
PfamiPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
TIGRFAMsiTIGR02042. sir. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSIR1_TOBAC
AccessioniPrimary (citable) accession number: O82802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: November 1, 1998
Last modified: September 7, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.