O82798 (ARR4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Two-component response regulator ARR4

Alternative name(s):
Response regulator 1

Gene names

Name:	ARR4
Synonyms:	ATRR1, IBC7
Ordered Locus Names:	At1g10470
ORF Names:	T10O24.8

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Type-A response regulators seem to act as negative regulators of the cytokinin signaling. Modulates red light signaling through its interaction with the phytochrome B photoreceptor. Ref.1 Ref.9 Ref.10
Subunit structure	Interacts with the phytochrome B photoreceptor. Binds to AHP1. Ref.7 Ref.9
Subcellular location	Nucleus Probable.
Tissue specificity	Predominantly expressed in roots, and stems of young inflorescences. Ref.1 Ref.2 Ref.3
Induction	By low temperature, dehydration, abscisic acid (ABA), cytokinins (BA and zeatin), nitrate and high salinity. Ref.2 Ref.3 Ref.6 Ref.8
Post-translational modification	Two-component system major event consists of a His-to-Asp phosphorelay between a sensor histidine kinase (HK) and a response regulator (RR). In plants, the His-to-Asp phosphorelay involves an additional intermediate named Histidine-containing phosphotransfer protein (HPt). This multistep phosphorelay consists of a His-Asp-His-Asp sequential transfer of a phosphate group between first an His and an Asp of the HK protein, followed by the transfer to a conserved His of the HPt protein and finally the transfer to an Asp in the receiver domain of the RR protein.
Sequence similarities	Belongs to the ARR family. Type-A subfamily. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Cytokinin signaling pathway Transcription Transcription regulation Two-component regulatory system
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	circadian rhythm Inferred from mutant phenotype. Source: TAIR cytokinin mediated signaling pathway Inferred from mutant phenotype. Source: TAIR embryo development ending in seed dormancy Inferred from mutant phenotype. Source: TAIR protein autophosphorylation Inferred from direct assay. Source: TAIR red or far-red light signaling pathway Inferred from mutant phenotype Ref.9. Source: TAIR regulation of circadian rhythm Inferred from genetic interaction. Source: TAIR regulation of transcription, DNA-dependent Traceable author statement. Source: TAIR response to red light Inferred from mutant phenotype Ref.10. Source: TAIR transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay Ref.9. Source: TAIR nucleus Inferred from direct assay Ref.9. Source: TAIR
Molecular function	protein binding Inferred from physical interaction Ref.7. Source: UniProtKB protein serine/threonine kinase activity Inferred from direct assay. Source: TAIR two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DBP	O80837	3	EBI-625213,EBI-1788073
PHYB	P14713	3	EBI-625213,EBI-300727

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Two-component response regulator ARR4		PRO_0000081425
Regions
	Domain	35 – 162	128	Response regulatory
	Compositional bias	179 – 246	68	Ser-rich
Amino acid modifications
	Modified residue	95	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O82798 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 557A4E2DA51948F5	FASTA	259	28,297
	10 20 30 40 50 60 MARDGGVSCL RRSEMMSVGG IGGIESAPLD LDEVHVLAVD DSLVDRIVIE RLLRITSCKV 70 80 90 100 110 120 TAVDSGWRAL EFLGLDNEKA SAEFDRLKVD LIITDYCMPG MTGYELLKKI KESSNFREVP 130 140 150 160 170 180 VVIMSSENVL TRIDRCLEEG AQDFLLKPVK LADVKRLRSH LTKDVKLSNG NKRKLPEDSS 190 200 210 220 230 240 SVNSSLPPPS PPLTISPESS PPLTVSTESS DSSPPLSPVE IFSTSPLSSP IDDEDDDVLT 250 SSSEESPIRR QKMRSPGLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Response regulators implicated in His-to-Asp phosphotransfer signaling in Arabidopsis." Imamura A., Hanaki N., Umeda H., Nakamura A., Suzuki T., Ueguchi C., Mizuno T. Proc. Natl. Acad. Sci. U.S.A. 95:2691-2696(1998) [PubMed: 9482949] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Stress-responsive expression of genes for two-component response regulator-like proteins in Arabidopsis thaliana." Urao T., Yakubov B., Yamaguchi-Shinozaki K., Shinozaki K. FEBS Lett. 427:175-178(1998) [PubMed: 9607306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Columbia.
[3]	"Two genes with similarity to bacterial response regulators are rapidly and specifically induced by cytokinin in Arabidopsis." Brandstatter I., Kieber J.J. Plant Cell 10:1009-1019(1998) [PubMed: 9634588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Wassilewskija.
[4]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[6]	"Expression of Arabidopsis response regulator homologs is induced by cytokinins and nitrate." Taniguchi M., Kiba T., Sakakibara H., Ueguchi C., Mizuno T., Sugiyama T. FEBS Lett. 429:259-262(1998) [PubMed: 9662428] [Abstract] Cited for: INDUCTION.
[7]	"Possible His to Asp phosphorelay signaling in an Arabidopsis two-component system." Urao T., Miyata S., Yamaguchi-Shinozaki K., Shinozaki K. FEBS Lett. 478:227-232(2000) [PubMed: 10930573] [Abstract] Cited for: INTERACTION WITH AHP1.
[8]	"Characterization of the response of the Arabidopsis response regulator gene family to cytokinin." D'Agostino I.B., Deruere J., Kieber J.J. Plant Physiol. 124:1706-1717(2000) [PubMed: 11115887] [Abstract] Cited for: INDUCTION.
[9]	"Interaction of the response regulator ARR4 with phytochrome B in modulating red light signaling." Sweere U., Eichenberg K., Lohrmann J., Mira-Rodado V., Baeurle I., Kudla J., Nagy F., Schaefer E., Harter K. Science 294:1108-1111(2001) [PubMed: 11691995] [Abstract] Cited for: FUNCTION, INTERACTION WITH PHYTOCHROME B.
[10]	"Type-A Arabidopsis response regulators are partially redundant negative regulators of cytokinin signaling." To J.P.C., Haberer G., Ferreira F.J., Deruere J., Mason M.G., Schaller G.E., Alonso J.M., Ecker J.R., Kieber J.J. Plant Cell 16:658-671(2004) [PubMed: 14973166] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB008487 mRNA. Translation: BAA34726.1. AB010915 mRNA. Translation: BAA31143.1. AF057282 mRNA. Translation: AAC26636.1. AC007067 Genomic DNA. Translation: AAD39568.1. CP002684 Genomic DNA. Translation: AEE28583.1.
IPI	IPI00523637.
PIR	T48851.
RefSeq	NP_172517.1. NM_100921.2.
UniGene	At.22981. At.67259.
3D structure databases
ProteinModelPortal	O82798.
SMR	O82798. Positions 34-161.
ModBase	Search...
Protein-protein interaction databases
IntAct	O82798. 7 interactions.
STRING	O82798.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G10470.1; AT1G10470.1; AT1G10470.
GeneID	837587.
GenomeReviews	Gene locus AT1G10470 in contig CT485782_GR.
KEGG	ath:AT1G10470.
Organism-specific databases
GeneFarm	4121. 418.
TAIR	At1g10470.
Phylogenomic databases
eggNOG	KOG1601.
InParanoid	O82798.
OMA	TISPESS.
PhylomeDB	O82798.
ProtClustDB	CLSN2912722.
Gene expression databases
ArrayExpress	O82798.
Genevestigator	O82798.
GermOnline	AT1G10470. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011006. CheY-like_superfamily. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
KO	K14492.
Pfam	PF00072. Response_reg. 1 hit. [Graphical view]
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52172. CheY_like. 1 hit.
PROSITE	PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARR4_ARATH

Accession

Primary (citable) accession number: O82798

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	November 1, 1998
Last modified:	December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program