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Protein

Phosphoserine phosphatase, chloroplastic

Gene

PSP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the last step in the plastidial phosphorylated pathway of serine biosynthesis (PPSB). The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates. Required for embryo, pollen and root development. May be required preferentially for serine biosynthesis in non-photosynthetic tissues.2 Publications

Catalytic activityi

O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Kineticsi

Approximately 60% inhibition of PSP activity by 10 mM serine was observed.
  1. KM=3.5 mM for 3-phosphoserine (at 30 degrees Celsius and pH 7.5)1 Publication

    Pathwayi: L-serine biosynthesis

    This protein is involved in step 3 of the subpathway that synthesizes L-serine from 3-phospho-D-glycerate.
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. D-3-phosphoglycerate dehydrogenase 2, chloroplastic (PGDH2), D-3-phosphoglycerate dehydrogenase (AXX17_At3g20680), D-3-phosphoglycerate dehydrogenase 1, chloroplastic (PGDH1), D-3-phosphoglycerate dehydrogenase 3, chloroplastic (PGDH3), D-3-phosphoglycerate dehydrogenase (AXX17_At1g18640), D-3-phosphoglycerate dehydrogenase (AXX17_At4g39120)
    2. Phosphoserine aminotransferase 1, chloroplastic (PSAT1), Phosphoserine aminotransferase (AXX17_At2g12910), Phosphoserine aminotransferase 2, chloroplastic (PSAT2), Phosphoserine aminotransferase (AXX17_At4g40680)
    3. Phosphoserine phosphatase, chloroplastic (PSP)
    This subpathway is part of the pathway L-serine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-serine from 3-phospho-D-glycerate, the pathway L-serine biosynthesis and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei89NucleophileBy similarity1
    Metal bindingi89MagnesiumBy similarity1
    Active sitei91Proton donorBy similarity1
    Metal bindingi91Magnesium; via carbonyl oxygenBy similarity1
    Binding sitei98SubstrateBy similarity1
    Binding sitei134SubstrateBy similarity1
    Binding sitei227SubstrateBy similarity1
    Metal bindingi248MagnesiumBy similarity1

    GO - Molecular functioni

    • calcium ion binding Source: UniProtKB
    • magnesium ion binding Source: UniProtKB
    • phosphoserine phosphatase activity Source: TAIR

    GO - Biological processi

    • embryo development ending in seed dormancy Source: TAIR
    • L-serine biosynthetic process Source: TAIR
    • L-serine metabolic process Source: UniProtKB
    • pollen development Source: TAIR
    • root development Source: TAIR

    Keywordsi

    Molecular functionHydrolase
    Biological processAmino-acid biosynthesis, Serine biosynthesis
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G18640-MONOMER
    MetaCyc:AT1G18640-MONOMER
    BRENDAi3.1.3.3 399
    ReactomeiR-ATH-977347 Serine biosynthesis
    UniPathwayiUPA00135; UER00198

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoserine phosphatase, chloroplastic (EC:3.1.3.3)
    Short name:
    PSP
    Short name:
    PSPase
    Alternative name(s):
    O-phosphoserine phosphohydrolase
    Gene namesi
    Name:PSP
    Synonyms:PSP1
    Ordered Locus Names:At1g18640
    ORF Names:F26I16.2
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G18640
    TAIRilocus:2027433 AT1G18640

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Embryo lethal when homozygous.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 62ChloroplastSequence analysisAdd BLAST62
    ChainiPRO_000003237563 – 295Phosphoserine phosphatase, chloroplasticAdd BLAST233

    Proteomic databases

    PaxDbiO82796
    PRIDEiO82796

    Expressioni

    Tissue specificityi

    Ubiquitous. Mainly expressed in shoot and root meristems, vasculature, pollen, anthers, carpels and seeds.1 Publication

    Inductioni

    Up-regulated in aerial parts by 8 hours exposure to darkness, whereas longer exposure down-regulate expression in both roots and aerial parts.1 Publication

    Gene expression databases

    ExpressionAtlasiO82796 differential
    GenevisibleiO82796 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi23684, 1 interactor
    IntActiO82796, 1 interactor
    STRINGi3702.AT1G18640.2

    Structurei

    3D structure databases

    ProteinModelPortaliO82796
    SMRiO82796
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni178 – 179Substrate bindingBy similarity2

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG1615 Eukaryota
    COG0560 LUCA
    HOGENOMiHOG000231116
    InParanoidiO82796
    KOiK01079
    OMAiNGSYAGF
    OrthoDBiEOG09360HM9
    PhylomeDBiO82796

    Family and domain databases

    Gene3Di1.10.150.210, 2 hits
    3.40.50.1000, 3 hits
    InterProiView protein in InterPro
    IPR036412 HAD-like_sf
    IPR023214 HAD_sf
    IPR023190 Pser_Pase_dom_2
    SUPFAMiSSF56784 SSF56784, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O82796-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEALTTSRVV PVQVPCRKLS SLFANFSCLE LRRYPCRGLV SIMNHPKLLR
    60 70 80 90 100
    PVTASVQPHE LSTLGHEGNI VPSKEILDLW RSVEAVCFDV DSTVCVDEGI
    110 120 130 140 150
    DELAEFCGAG KAVAEWTARA MGGSVPFEEA LAARLSLFKP SLSKVEEYLD
    160 170 180 190 200
    KRPPRLSPGI EELVKKLRAN NIDVYLISGG FRQMINPVAS ILGIPRENIF
    210 220 230 240 250
    ANNLLFGNSG EFLGFDENEP TSRSGGKAKA VQQIRKGRLY KTMAMIGDGA
    260 270 280 290
    TDLEARKPGG ADLFICYAGV QLREAVAANA DWLIFKFESL INSLD
    Length:295
    Mass (Da):32,318
    Last modified:April 26, 2005 - v2
    Checksum:iF14C95E636F7745E
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti266C → S in BAA33806 (PubMed:10196182).Curated1
    Sequence conflicti266C → S in BAA33807 (PubMed:10196182).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB018408 mRNA Translation: BAA33806.1
    AB018409 Genomic DNA Translation: BAA33807.1
    AC026238 Genomic DNA Translation: AAF98410.1
    CP002684 Genomic DNA Translation: AEE29738.1
    AY065351 mRNA Translation: AAL38792.1
    AY096687 mRNA Translation: AAM20321.1
    AY087385 mRNA Translation: AAM64935.1
    PIRiB86320
    T51362
    RefSeqiNP_973858.1, NM_202129.3
    UniGeneiAt.356
    At.41785

    Genome annotation databases

    EnsemblPlantsiAT1G18640.2; AT1G18640.2; AT1G18640
    GeneIDi838445
    GrameneiAT1G18640.2; AT1G18640.2; AT1G18640
    KEGGiath:AT1G18640

    Similar proteinsi

    Entry informationi

    Entry nameiSERC_ARATH
    AccessioniPrimary (citable) accession number: O82796
    Secondary accession number(s): Q9FZ85
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: April 26, 2005
    Last modified: May 23, 2018
    This is version 128 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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