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O82784

- MDL4_PRUSE

UniProt

O82784 - MDL4_PRUSE

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Protein

(R)-mandelonitrile lyase 4

Gene
MDL4
Organism
Prunus serotina (Black cherry)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in cyanogenesis, the release of HCN from injured tissues. Catalyzes the stereospecific addition of HCN to a variety of aldehydes in vitro. It is a major seed constituent, and could have the additional role of a storage form for reduced nitrogen By similarity.

Catalytic activityi

(R)-mandelonitrile = cyanide + benzaldehyde.

Cofactori

FAD By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei134 – 1341FAD By similarity
Binding sitei245 – 2451FAD; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei357 – 3571Substrate By similarity
Binding sitei486 – 4861Substrate By similarity
Active sitei488 – 4881Proton donor By similarity
Binding sitei516 – 5161FAD; via amide nitrogen By similarity
Active sitei526 – 5261Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 652FAD By similarity
Nucleotide bindingi83 – 842FAD By similarity
Nucleotide bindingi138 – 1414FAD By similarity
Nucleotide bindingi487 – 4882FAD By similarity
Nucleotide bindingi527 – 5282FAD By similarity

GO - Molecular functioni

  1. choline dehydrogenase activity Source: InterPro
  2. flavin adenine dinucleotide binding Source: InterPro
  3. mandelonitrile lyase activity Source: UniProtKB-EC

GO - Biological processi

  1. alcohol metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
(R)-mandelonitrile lyase 4 (EC:4.1.2.10)
Alternative name(s):
Hydroxynitrile lyase 4
Short name:
(R)-oxynitrilase 4
Gene namesi
Name:MDL4
OrganismiPrunus serotina (Black cherry)
Taxonomic identifieri23207 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeMaloideaeAmygdaleaePrunus

Subcellular locationi

Vacuolealeurone grain By similarity
Note: Primarily found within protein bodies of the cotyledonary parenchyma cells, with lesser amounts within the procambium By similarity.

GO - Cellular componenti

  1. aleurone grain Source: UniProtKB-SubCell
  2. vacuole Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed predictionAdd
BLAST
Chaini28 – 574547(R)-mandelonitrile lyase 4PRO_0000012343Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi30 – 301N-linked (GlcNAc...) Reviewed prediction
Glycosylationi76 – 761N-linked (GlcNAc...) Reviewed prediction
Glycosylationi146 – 1461N-linked (GlcNAc...) Reviewed prediction
Glycosylationi151 – 1511N-linked (GlcNAc...) Reviewed prediction
Glycosylationi179 – 1791N-linked (GlcNAc...) Reviewed prediction
Glycosylationi268 – 2681N-linked (GlcNAc...) Reviewed prediction
Glycosylationi310 – 3101N-linked (GlcNAc...) Reviewed prediction
Glycosylationi381 – 3811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi407 – 4071N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi428 ↔ 479 By similarity
Glycosylationi468 – 4681N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Monomer By similarity.

Structurei

3D structure databases

ProteinModelPortaliO82784.
SMRiO82784. Positions 31-549.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi294 – 2974Poly-Leu

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
[Graphical view]
PfamiPF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000137. Alcohol_oxidase. 1 hit.
PROSITEiPS00624. GMC_OXRED_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O82784-1 [UniParc]FASTAAdd to Basket

« Hide

MEKSTMSAVV LVLNLLVLHL QYSEVHSLAN TSSEHDFGYL KFVYNAVDLE    50
LEGSYDYIIV GGGTSGCPLA ATLSANYSVL VLERGTIATE YPNTLTVDGF 100
AYNLQQQDDG KTPVERFVSE DGIDNVRSRI LGGTTIINAG VYARANESFY 150
NNSGVEWDLD LVNEAYEWVE DAIVYKPSNQ SWQSITGTAF LEAGVHPDNG 200
FGLVHEEGTR LTGSTFDNSG TRHASDELLN KGDPDNLKVA VEAAVQKIIF 250
STESSGLTAV GVVYTDSNGT SHRALVSGKG EVILSAGTLG TPQLLLLSGV 300
GPESYLTSLN ISVVASHPYV GQYVNDNPRN FINILPPNPI EPSTVTVLGI 350
TSDFYQCSLS SLPFDTPPFS LFPTTSYPLP NQTFAHIVSK VPGPLSAGSL 400
TLQSSSNVSV APNVKFNYCS DPVDLTHCVS GMKKIGVFLS TDALKPYKVD 450
DLPGIDGFNI LGTPLPENQT DDAAFEKFCR DTVASYWHYH GGAIVGKVID 500
GNFRVTGINA LRVVDGSTFP ATPASHPQGF YLMLGRYVGT KIVQERSASG 550
EAIHTSTFKP KLMDSLKSAL SFAF 574
Length:574
Mass (Da):61,682
Last modified:November 1, 1998 - v1
Checksum:iBCDFAE1DC5D2C539
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF043186 mRNA. Translation: AAD02265.1.
AF043187 Genomic DNA. Translation: AAD02266.1.
AF053884 mRNA. Translation: AAC61980.1.
AF053885 Genomic DNA. Translation: AAC61981.1.
PIRiT50766.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF043186 mRNA. Translation: AAD02265.1 .
AF043187 Genomic DNA. Translation: AAD02266.1 .
AF053884 mRNA. Translation: AAC61980.1 .
AF053885 Genomic DNA. Translation: AAC61981.1 .
PIRi T50766.

3D structure databases

ProteinModelPortali O82784.
SMRi O82784. Positions 31-549.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
[Graphical view ]
Pfami PF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000137. Alcohol_oxidase. 1 hit.
PROSITEi PS00624. GMC_OXRED_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Hu Z., Poulton J.E.
    Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Entry informationi

Entry nameiMDL4_PRUSE
AccessioniPrimary (citable) accession number: O82784
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1998
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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