O82782 (HIS4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic
EC=5.3.1.16

Alternative name(s):
5-proFAR isomerase
BBM II
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Gene names

Name:	HIS6
Ordered Locus Names:	At2g36230
ORF Names:	F2H17.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxyribulos-1-ylamino)methylideneamino)-1-(5-phosphoribosyl)imidazole-4-carboxamide.
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the HisA/HisF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histidine biosynthetic process Inferred from direct assay Ref.1. Source: TAIR
Cellular_component	chloroplast Inferred from direct assay PubMed 18431481. Source: TAIR
Molecular_function	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Inferred from direct assay Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 304		1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic		PRO_0000013445

Sequences

	Sequence		Length	Mass (Da)
	O82782 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: AB17690E4635721B	FASTA	304	33,365
	10 20 30 40 50 60 MRTLSSQLYS NGGLTWFQKK NQSSLFIKHL RVSKPSRVQL ISAVQFRPCI DIHKGKVKQI 70 80 90 100 110 120 VGSTLRDLKE DGSVLVTNFE SDKSAEEYAK MYKEDGLTGG HVIMLGADPL SQAAAIGALH 130 140 150 160 170 180 AYPGGLQVGG GINSENCMSY IEEGASHVIV TSYVFNNGKI DLERLKDIVS IVGKQRLILD 190 200 210 220 230 240 LSCRKKDGRY AIVTDRWQKF SDVILDEKSL EFLGGFSDEF LVHGVDVEGK KLGIDEELVA 250 260 270 280 290 300 LLGNYSPIPV TYAGGVTVMD DVERIKDAGK GRVDVTVGSA LDIFGGNLPY KDVVAWHHKQ HSLH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of the gene encoding N'-[(5'-phosphoribosyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide (BBM II) isomerase from Arabidopsis thaliana." Fujimori K., Tada S., Kanai S., Ohta D. Mol. Gen. Genet. 259:216-223(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB008929 Genomic DNA. Translation: BAA32457.1. AB006139 mRNA. Translation: BAA32456.1. AC006921 Genomic DNA. Translation: AAD21442.1. CP002685 Genomic DNA. Translation: AEC09219.1. AK118659 mRNA. Translation: BAC43255.1. AY140058 mRNA. Translation: AAM98199.1. BT002552 mRNA. Translation: AAO00912.1.
IPI	IPI00548600.
PIR	T51822.
RefSeq	NP_181165.1. NM_129181.3.
UniGene	At.20166. At.66347.
3D structure databases
ProteinModelPortal	O82782.
SMR	O82782. Positions 45-300.
ModBase	Search...
Protein-protein interaction databases
IntAct	O82782. 1 interaction.
STRING	3702.AT2G36230.1-P.
Proteomic databases
PaxDb	O82782.
PRIDE	O82782.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G36230.1; AT2G36230.1; AT2G36230.
GeneID	818195.
KEGG	ath:AT2G36230.
Organism-specific databases
GeneFarm	4312.
TAIR	At2g36230.
Phylogenomic databases
eggNOG	COG0106.
HOGENOM	HOG000172261.
InParanoid	O82782.
KO	K01814.
OMA	VAMNKWQ.
PhylomeDB	O82782.
ProtClustDB	PLN02446.
Enzyme and pathway databases
BioCyc	MetaCyc:AT2G36230-MONOMER.
UniPathway	UPA00031; UER00009.
Gene expression databases
ArrayExpress	O82782.
Genevestigator	O82782.
GermOnline	AT2G36230. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR006062. His_biosynth. IPR011858. HisA_euk. IPR011060. RibuloseP-bd_barrel. [Graphical view]
PANTHER	PTHR21169. PTHR21169. 1 hit.
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR02129. hisA_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS4_ARATH

Accession

Primary (citable) accession number: O82782

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program