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Protein

1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic

Gene

HISN3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide.

Pathwayi: L-histidine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase 1, chloroplastic (HISN1A), ATP phosphoribosyltransferase 2, chloroplastic (HISN1B)
  2. Histidine biosynthesis bifunctional protein hisIE, chloroplastic (HISN2)
  3. Histidine biosynthesis bifunctional protein hisIE, chloroplastic (HISN2)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic (At2g36230), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic, 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic (HISN3), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic (AXX17_At2g32880)
  5. Imidazole glycerol phosphate synthase hisHF (AXX17_At4g31050), Imidazole glycerol phosphate synthase hisHF, chloroplastic (HISN4)
  6. Imidazoleglycerol-phosphate dehydratase 2, chloroplastic (HISN5B), Imidazoleglycerol-phosphate dehydratase (HISN5B), Imidazoleglycerol-phosphate dehydratase (AXX17_At3g24180), Imidazoleglycerol-phosphate dehydratase (At3g22425), Imidazoleglycerol-phosphate dehydratase 1, chloroplastic (HISN5A), Imidazoleglycerol-phosphate dehydratase (HISN5B), Imidazoleglycerol-phosphate dehydratase (AXX17_At4g17260), Imidazoleglycerol-phosphate dehydratase (AXX17_At4g17260)
  7. Histidinol-phosphate aminotransferase 2, chloroplastic (HISN6B), Histidinol-phosphate aminotransferase 1, chloroplastic (HISN6A)
  8. Bifunctional phosphatase IMPL2, chloroplastic (HISN7)
  9. Histidinol dehydrogenase, chloroplastic (HISN8), Histidinol dehydrogenase, chloroplastic (AXX17_At5g63470)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • histidine biosynthetic process Source: TAIR
  • tryptophan biosynthetic process Source: GO_Central

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

BioCyciARA:AT2G36230-MONOMER
MetaCyc:AT2G36230-MONOMER
BRENDAi5.3.1.16 399
UniPathwayiUPA00031; UER00009

Names & Taxonomyi

Protein namesi
Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic (EC:5.3.1.16)
Alternative name(s):
5-proFAR isomerase
BBM II
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase
Protein ALBINO AND PALE GREEN 10
Protein HISTIDINE BIOSYNTHESIS 3
Gene namesi
Name:HISN3
Synonyms:APG10
Ordered Locus Names:At2g36230
ORF Names:F2H17.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G36230
TAIRilocus:2049470 AT2G36230

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 61ChloroplastSequence analysisAdd BLAST61
ChainiPRO_000001344562 – 3041-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplasticAdd BLAST243

Proteomic databases

PaxDbiO82782

Expressioni

Gene expression databases

ExpressionAtlasiO82782 baseline and differential
GenevisibleiO82782 AT

Interactioni

Protein-protein interaction databases

IntActiO82782, 1 interactor
STRINGi3702.AT2G36230.1

Structurei

3D structure databases

ProteinModelPortaliO82782
SMRiO82782
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HisA/HisF family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3055 Eukaryota
COG0106 LUCA
HOGENOMiHOG000172261
InParanoidiO82782
KOiK01814
OMAiFVAMNKW
OrthoDBiEOG09360GUG
PhylomeDBiO82782

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006062 His_biosynth
IPR011858 HisA_euk
IPR011060 RibuloseP-bd_barrel
PfamiView protein in Pfam
PF00977 His_biosynth, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
TIGRFAMsiTIGR02129 hisA_euk, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O82782-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTLSSQLYS NGGLTWFQKK NQSSLFIKHL RVSKPSRVQL ISAVQFRPCI
60 70 80 90 100
DIHKGKVKQI VGSTLRDLKE DGSVLVTNFE SDKSAEEYAK MYKEDGLTGG
110 120 130 140 150
HVIMLGADPL SQAAAIGALH AYPGGLQVGG GINSENCMSY IEEGASHVIV
160 170 180 190 200
TSYVFNNGKI DLERLKDIVS IVGKQRLILD LSCRKKDGRY AIVTDRWQKF
210 220 230 240 250
SDVILDEKSL EFLGGFSDEF LVHGVDVEGK KLGIDEELVA LLGNYSPIPV
260 270 280 290 300
TYAGGVTVMD DVERIKDAGK GRVDVTVGSA LDIFGGNLPY KDVVAWHHKQ

HSLH
Length:304
Mass (Da):33,365
Last modified:November 1, 1998 - v1
Checksum:iAB17690E4635721B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008929 Genomic DNA Translation: BAA32457.1
AB006139 mRNA Translation: BAA32456.1
AC006921 Genomic DNA Translation: AAD21442.1
CP002685 Genomic DNA Translation: AEC09219.1
AK118659 mRNA Translation: BAC43255.1
AY140058 mRNA Translation: AAM98199.1
BT002552 mRNA Translation: AAO00912.1
PIRiT51822
RefSeqiNP_181165.1, NM_129181.4
UniGeneiAt.20166
At.66347

Genome annotation databases

EnsemblPlantsiAT2G36230.1; AT2G36230.1; AT2G36230
GeneIDi818195
GrameneiAT2G36230.1; AT2G36230.1; AT2G36230
KEGGiath:AT2G36230

Similar proteinsi

Entry informationi

Entry nameiHIS3_ARATH
AccessioniPrimary (citable) accession number: O82782
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health