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Protein

Probable inactive beta-glucosidase 25

Gene

BGLU25

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531SubstrateBy similarity
Binding sitei202 – 2021SubstrateBy similarity
Active sitei203 – 2031Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciARA:AT3G03640-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable inactive beta-glucosidase 25
Short name:
AtBGLU25
Gene namesi
Name:BGLU25
Synonyms:GLUC
Ordered Locus Names:At3g03640
ORF Names:T12J13.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G03640.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 531507Probable inactive beta-glucosidase 25PRO_0000389587Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi222 ↔ 230By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO82772.
PRIDEiO82772.

Expressioni

Gene expression databases

GenevisibleiO82772. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G03640.1.

Structurei

3D structure databases

ProteinModelPortaliO82772.
SMRiO82772. Positions 31-514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni477 – 4782Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiO82772.
KOiK01188.
OMAiINEPYEF.
OrthoDBiEOG0936070K.
PhylomeDBiO82772.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O82772-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKAILFLG LFLVVIVSPI TVYGGAVCPA SSTFGRGSFP DGFLFGATTS
60 70 80 90 100
AFQHEGAAEE GGRGSSIWDS FTLKQHSESN NNLDGRLGVD FYHHYKEDVQ
110 120 130 140 150
LLKKLNMDAF RFSISWSRIF PHGKKDKGVS ETGVKFYNDL INELIANGVT
160 170 180 190 200
PLVTLFQWDV PQALEDEYGG FLSDRILEDF RDFAQFAFNK YGDRVKHWVT
210 220 230 240 250
INEPYEFSRG GYETGEKAPG RCSKYVNEKC VAGKSGHEVY TVSHNLLLAH
260 270 280 290 300
AEAVEEFRKC GKCTGGKIGI VQSPMWFEPY DKKSTSSPSE EIVKRAMDFT
310 320 330 340 350
LGWHMEPITH GDYPQAMKDV VGSRLPSFTP EQKEKLKGSY DFVGINYFTS
360 370 380 390 400
TFVAHTDNVN PEKPSWEADS RLQLHSNNVD GFKIGSQPAT AKYPVCADGL
410 420 430 440 450
RKVLKYIKEN YNDPEIIVTG NGYKETLEEK DVLPDALSDS NRKYYHMRHL
460 470 480 490 500
MALHGAVCED KVNVKGYFVS SLMDGLEWED GYKTRSGLYY VDYGHNMGRH
510 520 530
EKQSAKWLSK LLEKVPDTIQ SKVDSDSRKE L
Length:531
Mass (Da):59,807
Last modified:November 1, 1998 - v1
Checksum:i18EF2E2FDCB7C23B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF082157 mRNA. Translation: AAC31962.1.
AF082158 Genomic DNA. Translation: AAC32194.1.
AC009327 Genomic DNA. Translation: AAF03468.1.
CP002686 Genomic DNA. Translation: AEE73966.1.
PIRiT51956.
RefSeqiNP_187014.1. NM_111235.3.
UniGeneiAt.40944.
At.46185.

Genome annotation databases

EnsemblPlantsiAT3G03640.1; AT3G03640.1; AT3G03640.
GeneIDi821201.
GrameneiAT3G03640.1; AT3G03640.1; AT3G03640.
KEGGiath:AT3G03640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF082157 mRNA. Translation: AAC31962.1.
AF082158 Genomic DNA. Translation: AAC32194.1.
AC009327 Genomic DNA. Translation: AAF03468.1.
CP002686 Genomic DNA. Translation: AEE73966.1.
PIRiT51956.
RefSeqiNP_187014.1. NM_111235.3.
UniGeneiAt.40944.
At.46185.

3D structure databases

ProteinModelPortaliO82772.
SMRiO82772. Positions 31-514.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G03640.1.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbiO82772.
PRIDEiO82772.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G03640.1; AT3G03640.1; AT3G03640.
GeneIDi821201.
GrameneiAT3G03640.1; AT3G03640.1; AT3G03640.
KEGGiath:AT3G03640.

Organism-specific databases

TAIRiAT3G03640.

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiO82772.
KOiK01188.
OMAiINEPYEF.
OrthoDBiEOG0936070K.
PhylomeDBiO82772.

Enzyme and pathway databases

BioCyciARA:AT3G03640-MONOMER.

Miscellaneous databases

PROiO82772.

Gene expression databases

GenevisibleiO82772. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGL25_ARATH
AccessioniPrimary (citable) accession number: O82772
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 1, 1998
Last modified: September 7, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Lacks the conserved Glu residue involved in nucleophilic attack and essential for hydrolase activity. Its enzyme activity is therefore unsure.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.