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O82734 (PP18_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase PP1 isozyme 8

EC=3.1.3.16
Gene names
Name:TOPP8
Ordered Locus Names:At5g27840
ORF Names:T1G16.170
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 manganese ions per subunit By similarity.

Tissue specificity

Expressed in roots, rosettes and flowers.

Sequence similarities

Belongs to the PPP phosphatase family. PP-1 subfamily.

Caution

It is uncertain whether Met-1, Met-2, Met-5 or Met-8 is the initiator.

Sequence caution

The sequence AAM65377.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O82734-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 2 (identifier: O82734-2)

The sequence of this isoform differs from the canonical sequence as follows:
     312-324: DFHNRTLGYNLSA → VPKMGKS

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 324323Serine/threonine-protein phosphatase PP1 isozyme 8
PRO_0000058804

Sites

Active site1271Proton donor By similarity
Metal binding661Manganese 1 By similarity
Metal binding681Manganese 1 By similarity
Metal binding941Manganese 1 By similarity
Metal binding941Manganese 2 By similarity
Metal binding1261Manganese 2 By similarity
Metal binding1751Manganese 2 By similarity
Metal binding2501Manganese 2 By similarity

Natural variations

Alternative sequence312 – 32413DFHNR…YNLSA → VPKMGKS in isoform 2.
VSP_009007

Experimental info

Sequence conflict2561G → E in AAC39461. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 21, 2003. Version 3.
Checksum: AD6ECB37F8FBBFE0

FASTA32436,806
        10         20         30         40         50         60 
MMTSMEGMVE KGVLDDIIRR LLEGKGGKQV QLSESEIRQL CFNARQIFLS QPNLLDLHAP 

        70         80         90        100        110        120 
IRICGDIHGQ YQDLLRLFEY GGYPPSANYL FLGDYVDRGK QSLETICLLL AYKIRYPSKI 

       130        140        150        160        170        180 
YLLRGNHEDA KINRIYGFYD ECKRRFNVRL WKVFTDCFNC LPVAALIDEK ILCMHGGLSP 

       190        200        210        220        230        240 
DLDNLNQIRE IQRPIEIPDS GLLCDLLWSD PDQKIEGWAD SDRGISCTFG ADKVAEFLDK 

       250        260        270        280        290        300 
NDLDLICRGH QVVEDGYEFF AKRRLVTIFS APNYGGEFDN AGALLSVDES LVCSFEIMKP 

       310        320 
APASSSHPLK KDFHNRTLGY NLSA 

« Hide

Isoform 2 [UniParc].

Checksum: 5D621EECB642105A
Show »

FASTA31836,044

References

« Hide 'large scale' references
[1]"Molecular cloning and chromosomal mapping of type one serine/threonine protein phosphatases in Arabidopsis thaliana."
Lin Q., Li J., Smith R.D., Walker J.C.
Plant Mol. Biol. 37:471-481(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]"Arabidopsis PPP family of serine/threonine phosphatases."
Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U80922 Genomic DNA. Translation: AAC39461.1.
AC069556 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93734.1.
CP002688 Genomic DNA. Translation: AED93735.1.
AY081492 mRNA. Translation: AAM10054.1.
AY042854 mRNA. Translation: AAK68794.1.
AY087823 mRNA. Translation: AAM65377.1. Different initiation.
RefSeqNP_568501.3. NM_122666.4. [O82734-1]
NP_851085.1. NM_180754.3. [O82734-2]
UniGeneAt.20531.

3D structure databases

ProteinModelPortalO82734.
SMRO82734. Positions 14-301.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid18120. 4 interactions.
IntActO82734. 2 interactions.
MINTMINT-8390409.

Proteomic databases

PaxDbO82734.
PRIDEO82734.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G27840.2; AT5G27840.2; AT5G27840. [O82734-1]
GeneID832846.
KEGGath:AT5G27840.

Organism-specific databases

TAIRAT5G27840.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000172697.
InParanoidO82734.
KOK06269.
OMATEIPDSG.
PhylomeDBO82734.

Enzyme and pathway databases

BioCycARA:AT5G27840-MONOMER.
ARA:GQT-481-MONOMER.

Gene expression databases

GenevestigatorO82734.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePP18_ARATH
AccessionPrimary (citable) accession number: O82734
Secondary accession number(s): Q8LAG7, Q94B49
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 21, 2003
Last modified: June 11, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names