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O82734

- PP18_ARATH

UniProt

O82734 - PP18_ARATH

Protein

Serine/threonine-protein phosphatase PP1 isozyme 8

Gene

TOPP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 3 (21 Nov 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi66 – 661Manganese 1By similarity
    Metal bindingi68 – 681Manganese 1By similarity
    Metal bindingi94 – 941Manganese 1By similarity
    Metal bindingi94 – 941Manganese 2By similarity
    Metal bindingi126 – 1261Manganese 2By similarity
    Active sitei127 – 1271Proton donorBy similarity
    Metal bindingi175 – 1751Manganese 2By similarity
    Metal bindingi250 – 2501Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein serine/threonine phosphatase activity Source: TAIR

    GO - Biological processi

    1. dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G27840-MONOMER.
    ARA:GQT-481-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PP1 isozyme 8 (EC:3.1.3.16)
    Gene namesi
    Name:TOPP8
    Ordered Locus Names:At5g27840
    ORF Names:T1G16.170
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G27840.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 324323Serine/threonine-protein phosphatase PP1 isozyme 8PRO_0000058804Add
    BLAST

    Proteomic databases

    PaxDbiO82734.
    PRIDEiO82734.

    Expressioni

    Tissue specificityi

    Expressed in roots, rosettes and flowers.

    Gene expression databases

    GenevestigatoriO82734.

    Interactioni

    Protein-protein interaction databases

    BioGridi18120. 4 interactions.
    IntActiO82734. 2 interactions.
    MINTiMINT-8390409.

    Structurei

    3D structure databases

    ProteinModelPortaliO82734.
    SMRiO82734. Positions 14-301.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000172697.
    InParanoidiO82734.
    KOiK06269.
    OMAiTEIPDSG.
    PhylomeDBiO82734.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O82734-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMTSMEGMVE KGVLDDIIRR LLEGKGGKQV QLSESEIRQL CFNARQIFLS    50
    QPNLLDLHAP IRICGDIHGQ YQDLLRLFEY GGYPPSANYL FLGDYVDRGK 100
    QSLETICLLL AYKIRYPSKI YLLRGNHEDA KINRIYGFYD ECKRRFNVRL 150
    WKVFTDCFNC LPVAALIDEK ILCMHGGLSP DLDNLNQIRE IQRPIEIPDS 200
    GLLCDLLWSD PDQKIEGWAD SDRGISCTFG ADKVAEFLDK NDLDLICRGH 250
    QVVEDGYEFF AKRRLVTIFS APNYGGEFDN AGALLSVDES LVCSFEIMKP 300
    APASSSHPLK KDFHNRTLGY NLSA 324

    Note: No experimental confirmation available.

    Length:324
    Mass (Da):36,806
    Last modified:November 21, 2003 - v3
    Checksum:iAD6ECB37F8FBBFE0
    GO
    Isoform 2 (identifier: O82734-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         312-324: DFHNRTLGYNLSA → VPKMGKS

    Show »
    Length:318
    Mass (Da):36,044
    Checksum:i5D621EECB642105A
    GO

    Sequence cautioni

    The sequence AAM65377.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti256 – 2561G → E in AAC39461. (PubMed:9617814)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei312 – 32413DFHNR…YNLSA → VPKMGKS in isoform 2. 1 PublicationVSP_009007Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U80922 Genomic DNA. Translation: AAC39461.1.
    AC069556 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED93734.1.
    CP002688 Genomic DNA. Translation: AED93735.1.
    AY081492 mRNA. Translation: AAM10054.1.
    AY042854 mRNA. Translation: AAK68794.1.
    AY087823 mRNA. Translation: AAM65377.1. Different initiation.
    RefSeqiNP_568501.3. NM_122666.4. [O82734-1]
    NP_851085.1. NM_180754.3. [O82734-2]
    UniGeneiAt.20531.

    Genome annotation databases

    EnsemblPlantsiAT5G27840.2; AT5G27840.2; AT5G27840. [O82734-1]
    GeneIDi832846.
    KEGGiath:AT5G27840.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U80922 Genomic DNA. Translation: AAC39461.1 .
    AC069556 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED93734.1 .
    CP002688 Genomic DNA. Translation: AED93735.1 .
    AY081492 mRNA. Translation: AAM10054.1 .
    AY042854 mRNA. Translation: AAK68794.1 .
    AY087823 mRNA. Translation: AAM65377.1 . Different initiation.
    RefSeqi NP_568501.3. NM_122666.4. [O82734-1 ]
    NP_851085.1. NM_180754.3. [O82734-2 ]
    UniGenei At.20531.

    3D structure databases

    ProteinModelPortali O82734.
    SMRi O82734. Positions 14-301.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 18120. 4 interactions.
    IntActi O82734. 2 interactions.
    MINTi MINT-8390409.

    Proteomic databases

    PaxDbi O82734.
    PRIDEi O82734.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G27840.2 ; AT5G27840.2 ; AT5G27840 . [O82734-1 ]
    GeneIDi 832846.
    KEGGi ath:AT5G27840.

    Organism-specific databases

    TAIRi AT5G27840.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000172697.
    InParanoidi O82734.
    KOi K06269.
    OMAi TEIPDSG.
    PhylomeDBi O82734.

    Enzyme and pathway databases

    BioCyci ARA:AT5G27840-MONOMER.
    ARA:GQT-481-MONOMER.

    Gene expression databases

    Genevestigatori O82734.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and chromosomal mapping of type one serine/threonine protein phosphatases in Arabidopsis thaliana."
      Lin Q., Li J., Smith R.D., Walker J.C.
      Plant Mol. Biol. 37:471-481(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    6. "Arabidopsis PPP family of serine/threonine phosphatases."
      Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
      Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPP18_ARATH
    AccessioniPrimary (citable) accession number: O82734
    Secondary accession number(s): Q8LAG7, Q94B49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 21, 2003
    Last modified: October 1, 2014
    This is version 118 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3