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Protein

Serine/threonine-protein phosphatase PP1 isozyme 8

Gene

TOPP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi66Manganese 1By similarity1
Metal bindingi68Manganese 1By similarity1
Metal bindingi94Manganese 1By similarity1
Metal bindingi94Manganese 2By similarity1
Metal bindingi126Manganese 2By similarity1
Active sitei127Proton donorBy similarity1
Metal bindingi175Manganese 2By similarity1
Metal bindingi250Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 8 (EC:3.1.3.16)
Gene namesi
Name:TOPP8
Ordered Locus Names:At5g27840
ORF Names:T1G16.170
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G27840.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000588042 – 324Serine/threonine-protein phosphatase PP1 isozyme 8Add BLAST323

Proteomic databases

PaxDbiO82734.
PRIDEiO82734.

Expressioni

Tissue specificityi

Expressed in roots, rosettes and flowers.

Gene expression databases

GenevisibleiO82734. AT.

Interactioni

Protein-protein interaction databases

BioGridi18120. 6 interactors.
IntActiO82734. 2 interactors.
MINTiMINT-8390409.
STRINGi3702.AT5G27840.2.

Structurei

3D structure databases

ProteinModelPortaliO82734.
SMRiO82734.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IN81. Eukaryota.
ENOG410YCEC. LUCA.
HOGENOMiHOG000172697.
InParanoidiO82734.
KOiK06269.
OMAiTSMEGMV.
OrthoDBiEOG09360G9F.
PhylomeDBiO82734.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O82734-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMTSMEGMVE KGVLDDIIRR LLEGKGGKQV QLSESEIRQL CFNARQIFLS
60 70 80 90 100
QPNLLDLHAP IRICGDIHGQ YQDLLRLFEY GGYPPSANYL FLGDYVDRGK
110 120 130 140 150
QSLETICLLL AYKIRYPSKI YLLRGNHEDA KINRIYGFYD ECKRRFNVRL
160 170 180 190 200
WKVFTDCFNC LPVAALIDEK ILCMHGGLSP DLDNLNQIRE IQRPIEIPDS
210 220 230 240 250
GLLCDLLWSD PDQKIEGWAD SDRGISCTFG ADKVAEFLDK NDLDLICRGH
260 270 280 290 300
QVVEDGYEFF AKRRLVTIFS APNYGGEFDN AGALLSVDES LVCSFEIMKP
310 320
APASSSHPLK KDFHNRTLGY NLSA
Note: No experimental confirmation available.
Length:324
Mass (Da):36,806
Last modified:November 21, 2003 - v3
Checksum:iAD6ECB37F8FBBFE0
GO
Isoform 2 (identifier: O82734-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     312-324: DFHNRTLGYNLSA → VPKMGKS

Show »
Length:318
Mass (Da):36,044
Checksum:i5D621EECB642105A
GO

Sequence cautioni

The sequence AAM65377 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti256G → E in AAC39461 (PubMed:9617814).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009007312 – 324DFHNR…YNLSA → VPKMGKS in isoform 2. 1 PublicationAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80922 Genomic DNA. Translation: AAC39461.1.
AC069556 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93734.1.
CP002688 Genomic DNA. Translation: AED93735.1.
AY081492 mRNA. Translation: AAM10054.1.
AY042854 mRNA. Translation: AAK68794.1.
AY087823 mRNA. Translation: AAM65377.1. Different initiation.
RefSeqiNP_568501.3. NM_122666.4. [O82734-1]
NP_851085.1. NM_180754.4. [O82734-2]
UniGeneiAt.20531.

Genome annotation databases

EnsemblPlantsiAT5G27840.2; AT5G27840.2; AT5G27840. [O82734-1]
GeneIDi832846.
GrameneiAT5G27840.2; AT5G27840.2; AT5G27840.
KEGGiath:AT5G27840.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80922 Genomic DNA. Translation: AAC39461.1.
AC069556 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93734.1.
CP002688 Genomic DNA. Translation: AED93735.1.
AY081492 mRNA. Translation: AAM10054.1.
AY042854 mRNA. Translation: AAK68794.1.
AY087823 mRNA. Translation: AAM65377.1. Different initiation.
RefSeqiNP_568501.3. NM_122666.4. [O82734-1]
NP_851085.1. NM_180754.4. [O82734-2]
UniGeneiAt.20531.

3D structure databases

ProteinModelPortaliO82734.
SMRiO82734.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi18120. 6 interactors.
IntActiO82734. 2 interactors.
MINTiMINT-8390409.
STRINGi3702.AT5G27840.2.

Proteomic databases

PaxDbiO82734.
PRIDEiO82734.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G27840.2; AT5G27840.2; AT5G27840. [O82734-1]
GeneIDi832846.
GrameneiAT5G27840.2; AT5G27840.2; AT5G27840.
KEGGiath:AT5G27840.

Organism-specific databases

TAIRiAT5G27840.

Phylogenomic databases

eggNOGiENOG410IN81. Eukaryota.
ENOG410YCEC. LUCA.
HOGENOMiHOG000172697.
InParanoidiO82734.
KOiK06269.
OMAiTSMEGMV.
OrthoDBiEOG09360G9F.
PhylomeDBiO82734.

Miscellaneous databases

PROiO82734.

Gene expression databases

GenevisibleiO82734. AT.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPP18_ARATH
AccessioniPrimary (citable) accession number: O82734
Secondary accession number(s): Q8LAG7, Q94B49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 21, 2003
Last modified: November 30, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1, Met-2, Met-5 or Met-8 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.