O82734 (PP18_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 8
EC=3.1.3.16

Gene names

Name:	TOPP8
Ordered Locus Names:	At5g27840
ORF Names:	T1G16.170

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Tissue specificity	Expressed in roots, rosettes and flowers.
Sequence similarities	Belongs to the PPP phosphatase family. PP-1 subfamily.
Caution	It is uncertain whether Met-1, Met-2, Met-5 or Met-8 is the initiator.
Sequence caution	The sequence AAM65377.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from direct assay PubMed 21222654. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O82734-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: O82734-2)

The sequence of this isoform differs from the canonical sequence as follows:
     312-324: DFHNRTLGYNLSA → VPKMGKS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Serine/threonine-protein phosphatase PP1 isozyme 8		PRO_0000058804
Sites
	Active site	127	1	Proton donor By similarity
	Metal binding	66	1	Iron By similarity
	Metal binding	68	1	Iron By similarity
	Metal binding	94	1	Iron By similarity
	Metal binding	94	1	Manganese By similarity
	Metal binding	126	1	Manganese By similarity
	Metal binding	175	1	Manganese By similarity
	Metal binding	250	1	Manganese By similarity
Natural variations
	Alternative sequence	312 – 324	13	DFHNR…YNLSA → VPKMGKS in isoform 2.		VSP_009007
Experimental info
	Sequence conflict	256	1	G → E in AAC39461. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 21, 2003. Version 3. Checksum: AD6ECB37F8FBBFE0	FASTA	324	36,806
	10 20 30 40 50 60 MMTSMEGMVE KGVLDDIIRR LLEGKGGKQV QLSESEIRQL CFNARQIFLS QPNLLDLHAP 70 80 90 100 110 120 IRICGDIHGQ YQDLLRLFEY GGYPPSANYL FLGDYVDRGK QSLETICLLL AYKIRYPSKI 130 140 150 160 170 180 YLLRGNHEDA KINRIYGFYD ECKRRFNVRL WKVFTDCFNC LPVAALIDEK ILCMHGGLSP 190 200 210 220 230 240 DLDNLNQIRE IQRPIEIPDS GLLCDLLWSD PDQKIEGWAD SDRGISCTFG ADKVAEFLDK 250 260 270 280 290 300 NDLDLICRGH QVVEDGYEFF AKRRLVTIFS APNYGGEFDN AGALLSVDES LVCSFEIMKP 310 320 APASSSHPLK KDFHNRTLGY NLSA
	Isoform 2 [UniParc]. Checksum: 5D621EECB642105A	FASTA	318	36,044
	10 20 30 40 50 60 MMTSMEGMVE KGVLDDIIRR LLEGKGGKQV QLSESEIRQL CFNARQIFLS QPNLLDLHAP 70 80 90 100 110 120 IRICGDIHGQ YQDLLRLFEY GGYPPSANYL FLGDYVDRGK QSLETICLLL AYKIRYPSKI 130 140 150 160 170 180 YLLRGNHEDA KINRIYGFYD ECKRRFNVRL WKVFTDCFNC LPVAALIDEK ILCMHGGLSP 190 200 210 220 230 240 DLDNLNQIRE IQRPIEIPDS GLLCDLLWSD PDQKIEGWAD SDRGISCTFG ADKVAEFLDK 250 260 270 280 290 300 NDLDLICRGH QVVEDGYEFF AKRRLVTIFS APNYGGEFDN AGALLSVDES LVCSFEIMKP 310 APASSSHPLK KVPKMGKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and chromosomal mapping of type one serine/threonine protein phosphatases in Arabidopsis thaliana." Lin Q., Li J., Smith R.D., Walker J.C. Plant Mol. Biol. 37:471-481(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"Arabidopsis PPP family of serine/threonine phosphatases." Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C. Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U80922 Genomic DNA. Translation: AAC39461.1. AC069556 Genomic DNA. No translation available. CP002688 Genomic DNA. Translation: AED93734.1. CP002688 Genomic DNA. Translation: AED93735.1. AY081492 mRNA. Translation: AAM10054.1. AY042854 mRNA. Translation: AAK68794.1. AY087823 mRNA. Translation: AAM65377.1. Different initiation.
IPI	IPI00535872. IPI00540674.
RefSeq	NP_568501.3. NM_122666.4. NP_851085.1. NM_180754.3.
UniGene	At.20531.
3D structure databases
ProteinModelPortal	O82734.
SMR	O82734. Positions 14-301.
ModBase	Search...
Protein-protein interaction databases
IntAct	O82734. 1 interaction.
MINT	MINT-8390409.
Proteomic databases
PaxDb	O82734.
PRIDE	O82734.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G27840.2; AT5G27840.2; AT5G27840.
GeneID	832846.
KEGG	ath:AT5G27840.
Organism-specific databases
TAIR	At5g27840.
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HOG000172697.
InParanoid	O82734.
KO	K06269.
OMA	CSFEIMK.
PhylomeDB	O82734.
ProtClustDB	CLSN2684490.
Gene expression databases
Genevestigator	O82734.
GermOnline	AT5G27840. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP18_ARATH

Accession

Primary (citable) accession number: O82734
Secondary accession number(s): Q8LAG7, Q94B49

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 21, 2003
Last modified:	May 29, 2013
This is version 106 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program