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O82734

- PP18_ARATH

UniProt

O82734 - PP18_ARATH

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Protein

Serine/threonine-protein phosphatase PP1 isozyme 8

Gene
TOPP8, At5g27840, T1G16.170
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi66 – 661Manganese 1 By similarity
Metal bindingi68 – 681Manganese 1 By similarity
Metal bindingi94 – 941Manganese 1 By similarity
Metal bindingi94 – 941Manganese 2 By similarity
Metal bindingi126 – 1261Manganese 2 By similarity
Active sitei127 – 1271Proton donor By similarity
Metal bindingi175 – 1751Manganese 2 By similarity
Metal bindingi250 – 2501Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G27840-MONOMER.
ARA:GQT-481-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 8 (EC:3.1.3.16)
Gene namesi
Name:TOPP8
Ordered Locus Names:At5g27840
ORF Names:T1G16.170
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G27840.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 324323Serine/threonine-protein phosphatase PP1 isozyme 8PRO_0000058804Add
BLAST

Proteomic databases

PaxDbiO82734.
PRIDEiO82734.

Expressioni

Tissue specificityi

Expressed in roots, rosettes and flowers.

Gene expression databases

GenevestigatoriO82734.

Interactioni

Protein-protein interaction databases

BioGridi18120. 4 interactions.
IntActiO82734. 2 interactions.
MINTiMINT-8390409.

Structurei

3D structure databases

ProteinModelPortaliO82734.
SMRiO82734. Positions 14-301.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172697.
InParanoidiO82734.
KOiK06269.
OMAiTEIPDSG.
PhylomeDBiO82734.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O82734-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MMTSMEGMVE KGVLDDIIRR LLEGKGGKQV QLSESEIRQL CFNARQIFLS    50
QPNLLDLHAP IRICGDIHGQ YQDLLRLFEY GGYPPSANYL FLGDYVDRGK 100
QSLETICLLL AYKIRYPSKI YLLRGNHEDA KINRIYGFYD ECKRRFNVRL 150
WKVFTDCFNC LPVAALIDEK ILCMHGGLSP DLDNLNQIRE IQRPIEIPDS 200
GLLCDLLWSD PDQKIEGWAD SDRGISCTFG ADKVAEFLDK NDLDLICRGH 250
QVVEDGYEFF AKRRLVTIFS APNYGGEFDN AGALLSVDES LVCSFEIMKP 300
APASSSHPLK KDFHNRTLGY NLSA 324

Note: No experimental confirmation available.

Length:324
Mass (Da):36,806
Last modified:November 21, 2003 - v3
Checksum:iAD6ECB37F8FBBFE0
GO
Isoform 2 (identifier: O82734-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     312-324: DFHNRTLGYNLSA → VPKMGKS

Show »
Length:318
Mass (Da):36,044
Checksum:i5D621EECB642105A
GO

Sequence cautioni

The sequence AAM65377.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei312 – 32413DFHNR…YNLSA → VPKMGKS in isoform 2. VSP_009007Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti256 – 2561G → E in AAC39461. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U80922 Genomic DNA. Translation: AAC39461.1.
AC069556 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93734.1.
CP002688 Genomic DNA. Translation: AED93735.1.
AY081492 mRNA. Translation: AAM10054.1.
AY042854 mRNA. Translation: AAK68794.1.
AY087823 mRNA. Translation: AAM65377.1. Different initiation.
RefSeqiNP_568501.3. NM_122666.4. [O82734-1]
NP_851085.1. NM_180754.3. [O82734-2]
UniGeneiAt.20531.

Genome annotation databases

EnsemblPlantsiAT5G27840.2; AT5G27840.2; AT5G27840. [O82734-1]
GeneIDi832846.
KEGGiath:AT5G27840.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U80922 Genomic DNA. Translation: AAC39461.1 .
AC069556 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93734.1 .
CP002688 Genomic DNA. Translation: AED93735.1 .
AY081492 mRNA. Translation: AAM10054.1 .
AY042854 mRNA. Translation: AAK68794.1 .
AY087823 mRNA. Translation: AAM65377.1 . Different initiation.
RefSeqi NP_568501.3. NM_122666.4. [O82734-1 ]
NP_851085.1. NM_180754.3. [O82734-2 ]
UniGenei At.20531.

3D structure databases

ProteinModelPortali O82734.
SMRi O82734. Positions 14-301.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 18120. 4 interactions.
IntActi O82734. 2 interactions.
MINTi MINT-8390409.

Proteomic databases

PaxDbi O82734.
PRIDEi O82734.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G27840.2 ; AT5G27840.2 ; AT5G27840 . [O82734-1 ]
GeneIDi 832846.
KEGGi ath:AT5G27840.

Organism-specific databases

TAIRi AT5G27840.

Phylogenomic databases

eggNOGi COG0639.
HOGENOMi HOG000172697.
InParanoidi O82734.
KOi K06269.
OMAi TEIPDSG.
PhylomeDBi O82734.

Enzyme and pathway databases

BioCyci ARA:AT5G27840-MONOMER.
ARA:GQT-481-MONOMER.

Gene expression databases

Genevestigatori O82734.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and chromosomal mapping of type one serine/threonine protein phosphatases in Arabidopsis thaliana."
    Lin Q., Li J., Smith R.D., Walker J.C.
    Plant Mol. Biol. 37:471-481(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "Arabidopsis PPP family of serine/threonine phosphatases."
    Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
    Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPP18_ARATH
AccessioniPrimary (citable) accession number: O82734
Secondary accession number(s): Q8LAG7, Q94B49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 21, 2003
Last modified: June 11, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi