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Protein

Serine/threonine-protein phosphatase PP1 isozyme 8

Gene

TOPP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein phosphatase that possesses phosphatase activity toward para-nitrophenyl phosphate (pNPP) in vitro.1 Publication

Caution

It is uncertain whether Met-1, Met-2, Met-5 or Met-8 is the initiator.Curated

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Phosphatase activity is strongly reduced by the protein phosphatase inhibitor 2 (I-2).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi66Manganese 1By similarity1
Metal bindingi68Manganese 1By similarity1
Metal bindingi94Manganese 1By similarity1
Metal bindingi94Manganese 2By similarity1
Metal bindingi126Manganese 2By similarity1
Active sitei127Proton donorBy similarity1
Metal bindingi175Manganese 2By similarity1
Metal bindingi250Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 8Curated (EC:3.1.3.161 Publication)
Alternative name(s):
Type one protein phosphatase 81 Publication
Gene namesi
Name:TOPP81 Publication
Ordered Locus Names:At5g27840Imported
ORF Names:T1G16.170
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G27840
TAIRilocus:2180330 AT5G27840

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000588041 – 324Serine/threonine-protein phosphatase PP1 isozyme 8Add BLAST324

Proteomic databases

PaxDbiO82734
PRIDEiO82734

Expressioni

Tissue specificityi

Expressed in roots, rosettes and flowers.

Gene expression databases

ExpressionAtlasiO82734 baseline and differential
GenevisibleiO82734 AT

Interactioni

Protein-protein interaction databases

BioGridi18120, 6 interactors
IntActiO82734, 2 interactors
MINTiO82734
STRINGi3702.AT5G27840.2

Structurei

3D structure databases

ProteinModelPortaliO82734
SMRiO82734
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IN81 Eukaryota
ENOG410YCEC LUCA
HOGENOMiHOG000172697
InParanoidiO82734
KOiK06269
OMAiTSMEGMV
OrthoDBiEOG09360G9F
PhylomeDBiO82734

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR006186 Ser/Thr-sp_prot-phosphatase
IPR031675 STPPase_N
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF16891 STPPase_N, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O82734-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMTSMEGMVE KGVLDDIIRR LLEGKGGKQV QLSESEIRQL CFNARQIFLS
60 70 80 90 100
QPNLLDLHAP IRICGDIHGQ YQDLLRLFEY GGYPPSANYL FLGDYVDRGK
110 120 130 140 150
QSLETICLLL AYKIRYPSKI YLLRGNHEDA KINRIYGFYD ECKRRFNVRL
160 170 180 190 200
WKVFTDCFNC LPVAALIDEK ILCMHGGLSP DLDNLNQIRE IQRPIEIPDS
210 220 230 240 250
GLLCDLLWSD PDQKIEGWAD SDRGISCTFG ADKVAEFLDK NDLDLICRGH
260 270 280 290 300
QVVEDGYEFF AKRRLVTIFS APNYGGEFDN AGALLSVDES LVCSFEIMKP
310 320
APASSSHPLK KDFHNRTLGY NLSA
Note: No experimental confirmation available.
Length:324
Mass (Da):36,806
Last modified:November 21, 2003 - v3
Checksum:iAD6ECB37F8FBBFE0
GO
Isoform 2 (identifier: O82734-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     312-324: DFHNRTLGYNLSA → VPKMGKS

Show »
Length:318
Mass (Da):36,044
Checksum:i5D621EECB642105A
GO

Sequence cautioni

The sequence AAM65377 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti256G → E in AAC39461 (PubMed:9617814).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009007312 – 324DFHNR…YNLSA → VPKMGKS in isoform 2. 1 PublicationAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80922 Genomic DNA Translation: AAC39461.1
AC069556 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED93734.1
CP002688 Genomic DNA Translation: AED93735.1
CP002688 Genomic DNA Translation: ANM69190.1
AY081492 mRNA Translation: AAM10054.1
AY042854 mRNA Translation: AAK68794.1
AY087823 mRNA Translation: AAM65377.1 Different initiation.
RefSeqiNP_001330890.1, NM_001344042.1 [O82734-1]
NP_568501.3, NM_122666.4 [O82734-1]
NP_851085.1, NM_180754.4 [O82734-2]
UniGeneiAt.20531

Genome annotation databases

EnsemblPlantsiAT5G27840.1; AT5G27840.1; AT5G27840 [O82734-2]
AT5G27840.2; AT5G27840.2; AT5G27840 [O82734-1]
AT5G27840.3; AT5G27840.3; AT5G27840 [O82734-1]
GeneIDi832846
GrameneiAT5G27840.1; AT5G27840.1; AT5G27840 [O82734-2]
AT5G27840.2; AT5G27840.2; AT5G27840 [O82734-1]
AT5G27840.3; AT5G27840.3; AT5G27840 [O82734-1]
KEGGiath:AT5G27840

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPP18_ARATH
AccessioniPrimary (citable) accession number: O82734
Secondary accession number(s): Q8LAG7, Q94B49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 21, 2003
Last modified: April 25, 2018
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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