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O82733 (PP17_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase PP1 isozyme 7

EC=3.1.3.16
Gene names
Name:TOPP7
Ordered Locus Names:At5g43380
ORF Names:MWF20.7
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 manganese ions per subunit By similarity.

Tissue specificity

Expressed in roots, rosettes and flowers.

Sequence similarities

Belongs to the PPP phosphatase family. PP-1 subfamily.

Sequence caution

The sequence AAC39459.1 differs from that shown. Reason: Frameshift at position 314.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   LigandManganese
Metal-binding
   Molecular functionHydrolase
Protein phosphatase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoprotein phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O82733-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O82733-2)

The sequence of this isoform differs from the canonical sequence as follows:
     320-331: SILSSQNSKEYN → VCINHITF
Isoform 3 (identifier: O82733-3)

The sequence of this isoform differs from the canonical sequence as follows:
     320-331: SILSSQNSKEYN → DCNW
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331Serine/threonine-protein phosphatase PP1 isozyme 7
PRO_0000058803

Sites

Active site1211Proton donor By similarity
Metal binding601Manganese 1 By similarity
Metal binding621Manganese 1 By similarity
Metal binding881Manganese 1 By similarity
Metal binding881Manganese 2 By similarity
Metal binding1201Manganese 2 By similarity
Metal binding1691Manganese 2 By similarity
Metal binding2441Manganese 2 By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Natural variations

Alternative sequence320 – 33112SILSS…SKEYN → VCINHITF in isoform 2.
VSP_009006
Alternative sequence320 – 33112SILSS…SKEYN → DCNW in isoform 3.
VSP_028726

Experimental info

Sequence conflict71N → I in AAC39459. Ref.2
Sequence conflict761G → E in AAC39459. Ref.2
Sequence conflict841Missing in AAC39459. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 21, 2003. Version 3.
Checksum: 2D351A1C368B0CA2

FASTA33137,705
        10         20         30         40         50         60 
MDPGTLNSVI NRLLEAREKP GKIVQLSETE IKQLCFVSRD IFLRQPNLLE LEAPVKICGD 

        70         80         90        100        110        120 
IHGQYPDLLR LFEHGGYPPN SNYLFLGDYV DRGKQSLETI CLLLAYKIKF PENFFLLRGN 

       130        140        150        160        170        180 
HESASINRIY GFYDECKRRF SVKIWRIFTD CFNCLPVAAL IDERIFCMHG GLSPELLSLR 

       190        200        210        220        230        240 
QIRDIRRPTD IPDRGLLCDL LWSDPDKDVR GWGPNDRGVS YTFGSDIVSG FLKRLDLDLI 

       250        260        270        280        290        300 
CRAHQVVEDG FEFFANKQLV TIFSAPNYCG EFDNAGAMMS VSEDLTCSFQ ILKSNDKKSK 

       310        320        330 
FSFGSRGGAK TSFPYPKVKS ILSSQNSKEY N 

« Hide

Isoform 2 [UniParc].

Checksum: CBEEFA363F8C635A
Show »

FASTA32737,282
Isoform 3 [UniParc].

Checksum: F8B87342B8CC118B
Show »

FASTA32336,872

References

« Hide 'large scale' references
[1]"Isolation of a gene that encode a protein phosphatase 1 catalytic subunit in Arabidopsis thaliana."
Mitsukawa N., Okumura S., Shibata D.
Soil Sci. Plant Nutr. 43:971-974(1997)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
Strain: cv. Columbia.
[2]"Molecular cloning and chromosomal mapping of type one serine/threonine protein phosphatases in Arabidopsis thaliana."
Lin Q., Li J., Smith R.D., Walker J.C.
Plant Mol. Biol. 37:471-481(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[6]"Arabidopsis PPP family of serine/threonine phosphatases."
Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB000094 Genomic DNA. Translation: BAA24283.1.
U80920 mRNA. Translation: AAC39459.1. Frameshift.
AB025638 Genomic DNA. Translation: BAA97417.1.
CP002688 Genomic DNA. Translation: AED94952.1.
CP002688 Genomic DNA. Translation: AED94953.1.
CP002688 Genomic DNA. Translation: AED94954.1.
AY136464 mRNA. Translation: AAM97129.1.
BT002143 mRNA. Translation: AAN72154.1.
RefSeqNP_001032000.1. NM_001036923.1. [O82733-3]
NP_568625.1. NM_123704.2. [O82733-2]
NP_851123.1. NM_180792.2. [O82733-1]
UniGeneAt.1535.

3D structure databases

ProteinModelPortalO82733.
SMRO82733. Positions 6-293.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid19606. 3 interactions.
IntActO82733. 3 interactions.

Proteomic databases

PaxDbO82733.
PRIDEO82733.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G43380.1; AT5G43380.1; AT5G43380. [O82733-1]
GeneID834356.
KEGGath:AT5G43380.

Organism-specific databases

TAIRAT5G43380.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000172697.
InParanoidO82733.
KOK06269.
OMAGFEFFAN.
PhylomeDBO82733.

Enzyme and pathway databases

BioCycARA:AT5G43380-MONOMER.
ARA:GQT-2659-MONOMER.
ARA:GQT-2660-MONOMER.

Gene expression databases

GenevestigatorO82733.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePP17_ARATH
AccessionPrimary (citable) accession number: O82733
Secondary accession number(s): O48641, Q2V319, Q8L761
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 21, 2003
Last modified: June 11, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names