Serine/threonine-protein phosphatase PP1 isozyme 7 - Arabidopsis thaliana (Mouse-ear cress)

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O82733 (PP17_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 7
EC=3.1.3.16

Gene names

Name:	TOPP7
Ordered Locus Names:	At5g43380
ORF Names:	MWF20.7

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H₂O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Tissue specificity	Expressed in roots, rosettes and flowers.
Sequence similarities	Belongs to the PPP phosphatase family. PP-1 subfamily.
Sequence caution	The sequence AAC39459.1 differs from that shown. Reason: Frameshift at position 314.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O82733-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O82733-2) The sequence of this isoform differs from the canonical sequence as follows: 320-331: SILSSQNSKEYN → VCINHITF

Isoform 3 (identifier: O82733-3) The sequence of this isoform differs from the canonical sequence as follows: 320-331: SILSSQNSKEYN → DCNW
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 331

331

Serine/threonine-protein phosphatase PP1 isozyme 7

PRO_0000058803

Sites

Active site

121

Proton donor By similarity

Metal binding

Iron By similarity

Metal binding

Iron By similarity

Metal binding

Iron By similarity

Metal binding

Manganese By similarity

Metal binding

120

Manganese By similarity

Metal binding

169

Manganese By similarity

Metal binding

244

Manganese By similarity

Natural variations

Alternative sequence

320 – 331

SILSS…SKEYN → VCINHITF in isoform 2.

VSP_009006

Alternative sequence

320 – 331

SILSS…SKEYN → DCNW in isoform 3.

VSP_028726

Experimental info

Sequence conflict

N → I in AAC39459. Ref.2

Sequence conflict

G → E in AAC39459. Ref.2

Sequence conflict

Missing in AAC39459. Ref.2

Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified November 21, 2003. Version 3. Checksum: 2D351A1C368B0CA2 Show »	FASTA	331	37,705
10 20 30 40 50 60 MDPGTLNSVI NRLLEAREKP GKIVQLSETE IKQLCFVSRD IFLRQPNLLE LEAPVKICGD 70 80 90 100 110 120 IHGQYPDLLR LFEHGGYPPN SNYLFLGDYV DRGKQSLETI CLLLAYKIKF PENFFLLRGN 130 140 150 160 170 180 HESASINRIY GFYDECKRRF SVKIWRIFTD CFNCLPVAAL IDERIFCMHG GLSPELLSLR 190 200 210 220 230 240 QIRDIRRPTD IPDRGLLCDL LWSDPDKDVR GWGPNDRGVS YTFGSDIVSG FLKRLDLDLI 250 260 270 280 290 300 CRAHQVVEDG FEFFANKQLV TIFSAPNYCG EFDNAGAMMS VSEDLTCSFQ ILKSNDKKSK 310 320 330 FSFGSRGGAK TSFPYPKVKS ILSSQNSKEY N « Hide
Isoform 2 [UniParc]. Checksum: CBEEFA363F8C635A Show »	FASTA	327	37,282
10 20 30 40 50 60 MDPGTLNSVI NRLLEAREKP GKIVQLSETE IKQLCFVSRD IFLRQPNLLE LEAPVKICGD 70 80 90 100 110 120 IHGQYPDLLR LFEHGGYPPN SNYLFLGDYV DRGKQSLETI CLLLAYKIKF PENFFLLRGN 130 140 150 160 170 180 HESASINRIY GFYDECKRRF SVKIWRIFTD CFNCLPVAAL IDERIFCMHG GLSPELLSLR 190 200 210 220 230 240 QIRDIRRPTD IPDRGLLCDL LWSDPDKDVR GWGPNDRGVS YTFGSDIVSG FLKRLDLDLI 250 260 270 280 290 300 CRAHQVVEDG FEFFANKQLV TIFSAPNYCG EFDNAGAMMS VSEDLTCSFQ ILKSNDKKSK 310 320 FSFGSRGGAK TSFPYPKVKV CINHITF « Hide
Isoform 3 [UniParc]. Checksum: F8B87342B8CC118B Show »	FASTA	323	36,872
10 20 30 40 50 60 MDPGTLNSVI NRLLEAREKP GKIVQLSETE IKQLCFVSRD IFLRQPNLLE LEAPVKICGD 70 80 90 100 110 120 IHGQYPDLLR LFEHGGYPPN SNYLFLGDYV DRGKQSLETI CLLLAYKIKF PENFFLLRGN 130 140 150 160 170 180 HESASINRIY GFYDECKRRF SVKIWRIFTD CFNCLPVAAL IDERIFCMHG GLSPELLSLR 190 200 210 220 230 240 QIRDIRRPTD IPDRGLLCDL LWSDPDKDVR GWGPNDRGVS YTFGSDIVSG FLKRLDLDLI 250 260 270 280 290 300 CRAHQVVEDG FEFFANKQLV TIFSAPNYCG EFDNAGAMMS VSEDLTCSFQ ILKSNDKKSK 310 320 FSFGSRGGAK TSFPYPKVKD CNW « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a gene that encode a protein phosphatase 1 catalytic subunit in Arabidopsis thaliana." Mitsukawa N., Okumura S., Shibata D. Soil Sci. Plant Nutr. 43:971-974(1997) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2). Strain: cv. Columbia.
[2]	"Molecular cloning and chromosomal mapping of type one serine/threonine protein phosphatases in Arabidopsis thaliana." Lin Q., Li J., Smith R.D., Walker J.C. Plant Mol. Biol. 37:471-481(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[6]	"Arabidopsis PPP family of serine/threonine phosphatases." Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C. Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB000094 Genomic DNA. Translation: BAA24283.1. U80920 mRNA. Translation: AAC39459.1. Frameshift. AB025638 Genomic DNA. Translation: BAA97417.1. CP002688 Genomic DNA. Translation: AED94952.1. CP002688 Genomic DNA. Translation: AED94953.1. CP002688 Genomic DNA. Translation: AED94954.1. AY136464 mRNA. Translation: AAM97129.1. BT002143 mRNA. Translation: AAN72154.1.
IPI	IPI00518899. IPI00526896. IPI00656553.
RefSeq	NP_001032000.1. NM_001036923.1. NP_568625.1. NM_123704.2. NP_851123.1. NM_180792.2.
UniGene	At.1535.
3D structure databases
ProteinModelPortal	O82733.
SMR	O82733. Positions 6-293.
ModBase	Search...
Protein-protein interaction databases
IntAct	O82733. 3 interactions.
Proteomic databases
PaxDb	O82733.
PRIDE	O82733.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G43380.1; AT5G43380.1; AT5G43380.
GeneID	834356.
KEGG	ath:AT5G43380.
Organism-specific databases
TAIR	At5g43380.
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HOG000172697.
InParanoid	O82733.
KO	K06269.
OMA	GFEFFAN.
PhylomeDB	O82733.
ProtClustDB	CLSN2689922.
Gene expression databases
Genevestigator	O82733.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP17_ARATH

Accession

Primary (citable) accession number: O82733
Secondary accession number(s): O48641, Q2V319, Q8L761

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 21, 2003
Last modified:	May 1, 2013
This is version 107 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Sites

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents