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O82733

- PP17_ARATH

UniProt

O82733 - PP17_ARATH

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Protein
Serine/threonine-protein phosphatase PP1 isozyme 7
Gene
TOPP7, At5g43380, MWF20.7
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Manganese 1 By similarity
Metal bindingi62 – 621Manganese 1 By similarity
Metal bindingi88 – 881Manganese 1 By similarity
Metal bindingi88 – 881Manganese 2 By similarity
Metal bindingi120 – 1201Manganese 2 By similarity
Active sitei121 – 1211Proton donor By similarity
Metal bindingi169 – 1691Manganese 2 By similarity
Metal bindingi244 – 2441Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G43380-MONOMER.
ARA:GQT-2659-MONOMER.
ARA:GQT-2660-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 7 (EC:3.1.3.16)
Gene namesi
Name:TOPP7
Ordered Locus Names:At5g43380
ORF Names:MWF20.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G43380.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331Serine/threonine-protein phosphatase PP1 isozyme 7
PRO_0000058803Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO82733.
PRIDEiO82733.

Expressioni

Tissue specificityi

Expressed in roots, rosettes and flowers.

Gene expression databases

GenevestigatoriO82733.

Interactioni

Protein-protein interaction databases

BioGridi19606. 3 interactions.
IntActiO82733. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliO82733.
SMRiO82733. Positions 6-293.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172697.
InParanoidiO82733.
KOiK06269.
OMAiGFEFFAN.
PhylomeDBiO82733.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O82733-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDPGTLNSVI NRLLEAREKP GKIVQLSETE IKQLCFVSRD IFLRQPNLLE    50
LEAPVKICGD IHGQYPDLLR LFEHGGYPPN SNYLFLGDYV DRGKQSLETI 100
CLLLAYKIKF PENFFLLRGN HESASINRIY GFYDECKRRF SVKIWRIFTD 150
CFNCLPVAAL IDERIFCMHG GLSPELLSLR QIRDIRRPTD IPDRGLLCDL 200
LWSDPDKDVR GWGPNDRGVS YTFGSDIVSG FLKRLDLDLI CRAHQVVEDG 250
FEFFANKQLV TIFSAPNYCG EFDNAGAMMS VSEDLTCSFQ ILKSNDKKSK 300
FSFGSRGGAK TSFPYPKVKS ILSSQNSKEY N 331
Length:331
Mass (Da):37,705
Last modified:November 21, 2003 - v3
Checksum:i2D351A1C368B0CA2
GO
Isoform 2 (identifier: O82733-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     320-331: SILSSQNSKEYN → VCINHITF

Show »
Length:327
Mass (Da):37,282
Checksum:iCBEEFA363F8C635A
GO
Isoform 3 (identifier: O82733-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     320-331: SILSSQNSKEYN → DCNW

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:323
Mass (Da):36,872
Checksum:iF8B87342B8CC118B
GO

Sequence cautioni

The sequence AAC39459.1 differs from that shown. Reason: Frameshift at position 314.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei320 – 33112SILSS…SKEYN → VCINHITF in isoform 2.
VSP_009006Add
BLAST
Alternative sequencei320 – 33112SILSS…SKEYN → DCNW in isoform 3.
VSP_028726Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71N → I in AAC39459. 1 Publication
Sequence conflicti76 – 761G → E in AAC39459. 1 Publication
Sequence conflicti84 – 841Missing in AAC39459. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB000094 Genomic DNA. Translation: BAA24283.1.
U80920 mRNA. Translation: AAC39459.1. Frameshift.
AB025638 Genomic DNA. Translation: BAA97417.1.
CP002688 Genomic DNA. Translation: AED94952.1.
CP002688 Genomic DNA. Translation: AED94953.1.
CP002688 Genomic DNA. Translation: AED94954.1.
AY136464 mRNA. Translation: AAM97129.1.
BT002143 mRNA. Translation: AAN72154.1.
RefSeqiNP_001032000.1. NM_001036923.1. [O82733-3]
NP_568625.1. NM_123704.2. [O82733-2]
NP_851123.1. NM_180792.2. [O82733-1]
UniGeneiAt.1535.

Genome annotation databases

EnsemblPlantsiAT5G43380.1; AT5G43380.1; AT5G43380. [O82733-1]
GeneIDi834356.
KEGGiath:AT5G43380.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB000094 Genomic DNA. Translation: BAA24283.1 .
U80920 mRNA. Translation: AAC39459.1 . Frameshift.
AB025638 Genomic DNA. Translation: BAA97417.1 .
CP002688 Genomic DNA. Translation: AED94952.1 .
CP002688 Genomic DNA. Translation: AED94953.1 .
CP002688 Genomic DNA. Translation: AED94954.1 .
AY136464 mRNA. Translation: AAM97129.1 .
BT002143 mRNA. Translation: AAN72154.1 .
RefSeqi NP_001032000.1. NM_001036923.1. [O82733-3 ]
NP_568625.1. NM_123704.2. [O82733-2 ]
NP_851123.1. NM_180792.2. [O82733-1 ]
UniGenei At.1535.

3D structure databases

ProteinModelPortali O82733.
SMRi O82733. Positions 6-293.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 19606. 3 interactions.
IntActi O82733. 3 interactions.

Proteomic databases

PaxDbi O82733.
PRIDEi O82733.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G43380.1 ; AT5G43380.1 ; AT5G43380 . [O82733-1 ]
GeneIDi 834356.
KEGGi ath:AT5G43380.

Organism-specific databases

TAIRi AT5G43380.

Phylogenomic databases

eggNOGi COG0639.
HOGENOMi HOG000172697.
InParanoidi O82733.
KOi K06269.
OMAi GFEFFAN.
PhylomeDBi O82733.

Enzyme and pathway databases

BioCyci ARA:AT5G43380-MONOMER.
ARA:GQT-2659-MONOMER.
ARA:GQT-2660-MONOMER.

Gene expression databases

Genevestigatori O82733.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a gene that encode a protein phosphatase 1 catalytic subunit in Arabidopsis thaliana."
    Mitsukawa N., Okumura S., Shibata D.
    Soil Sci. Plant Nutr. 43:971-974(1997)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
    Strain: cv. Columbia.
  2. "Molecular cloning and chromosomal mapping of type one serine/threonine protein phosphatases in Arabidopsis thaliana."
    Lin Q., Li J., Smith R.D., Walker J.C.
    Plant Mol. Biol. 37:471-481(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "Arabidopsis PPP family of serine/threonine phosphatases."
    Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
    Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPP17_ARATH
AccessioniPrimary (citable) accession number: O82733
Secondary accession number(s): O48641, Q2V319, Q8L761
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 21, 2003
Last modified: June 11, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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