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Protein

Serine/threonine-protein phosphatase PP1 isozyme 7

Gene

TOPP7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60Manganese 1By similarity1
Metal bindingi62Manganese 1By similarity1
Metal bindingi88Manganese 1By similarity1
Metal bindingi88Manganese 2By similarity1
Metal bindingi120Manganese 2By similarity1
Active sitei121Proton donorBy similarity1
Metal bindingi169Manganese 2By similarity1
Metal bindingi244Manganese 2By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 7 (EC:3.1.3.16)
Gene namesi
Name:TOPP7
Ordered Locus Names:At5g43380
ORF Names:MWF20.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G43380.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000588031 – 331Serine/threonine-protein phosphatase PP1 isozyme 7Add BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO82733.

Expressioni

Tissue specificityi

Expressed in roots, rosettes and flowers.

Gene expression databases

GenevisibleiO82733. AT.

Interactioni

Protein-protein interaction databases

BioGridi19606. 4 interactors.
IntActiO82733. 3 interactors.
STRINGi3702.AT5G43380.1.

Structurei

3D structure databases

ProteinModelPortaliO82733.
SMRiO82733.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0374. Eukaryota.
COG0639. LUCA.
HOGENOMiHOG000172697.
InParanoidiO82733.
KOiK06269.
OMAiGFEFFAN.
OrthoDBiEOG09360FO9.
PhylomeDBiO82733.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O82733-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPGTLNSVI NRLLEAREKP GKIVQLSETE IKQLCFVSRD IFLRQPNLLE
60 70 80 90 100
LEAPVKICGD IHGQYPDLLR LFEHGGYPPN SNYLFLGDYV DRGKQSLETI
110 120 130 140 150
CLLLAYKIKF PENFFLLRGN HESASINRIY GFYDECKRRF SVKIWRIFTD
160 170 180 190 200
CFNCLPVAAL IDERIFCMHG GLSPELLSLR QIRDIRRPTD IPDRGLLCDL
210 220 230 240 250
LWSDPDKDVR GWGPNDRGVS YTFGSDIVSG FLKRLDLDLI CRAHQVVEDG
260 270 280 290 300
FEFFANKQLV TIFSAPNYCG EFDNAGAMMS VSEDLTCSFQ ILKSNDKKSK
310 320 330
FSFGSRGGAK TSFPYPKVKS ILSSQNSKEY N
Length:331
Mass (Da):37,705
Last modified:November 21, 2003 - v3
Checksum:i2D351A1C368B0CA2
GO
Isoform 2 (identifier: O82733-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     320-331: SILSSQNSKEYN → VCINHITF

Show »
Length:327
Mass (Da):37,282
Checksum:iCBEEFA363F8C635A
GO
Isoform 3 (identifier: O82733-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     320-331: SILSSQNSKEYN → DCNW

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:323
Mass (Da):36,872
Checksum:iF8B87342B8CC118B
GO

Sequence cautioni

The sequence AAC39459 differs from that shown. Reason: Frameshift at position 314.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7N → I in AAC39459 (PubMed:9617814).Curated1
Sequence conflicti76G → E in AAC39459 (PubMed:9617814).Curated1
Sequence conflicti84Missing in AAC39459 (PubMed:9617814).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009006320 – 331SILSS…SKEYN → VCINHITF in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_028726320 – 331SILSS…SKEYN → DCNW in isoform 3. CuratedAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000094 Genomic DNA. Translation: BAA24283.1.
U80920 mRNA. Translation: AAC39459.1. Frameshift.
AB025638 Genomic DNA. Translation: BAA97417.1.
CP002688 Genomic DNA. Translation: AED94952.1.
CP002688 Genomic DNA. Translation: AED94953.1.
CP002688 Genomic DNA. Translation: AED94954.1.
AY136464 mRNA. Translation: AAM97129.1.
BT002143 mRNA. Translation: AAN72154.1.
RefSeqiNP_001032000.1. NM_001036923.1. [O82733-3]
NP_568625.1. NM_123704.3. [O82733-2]
NP_851123.1. NM_180792.3. [O82733-1]
UniGeneiAt.1535.

Genome annotation databases

EnsemblPlantsiAT5G43380.1; AT5G43380.1; AT5G43380. [O82733-1]
GeneIDi834356.
GrameneiAT5G43380.1; AT5G43380.1; AT5G43380.
KEGGiath:AT5G43380.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000094 Genomic DNA. Translation: BAA24283.1.
U80920 mRNA. Translation: AAC39459.1. Frameshift.
AB025638 Genomic DNA. Translation: BAA97417.1.
CP002688 Genomic DNA. Translation: AED94952.1.
CP002688 Genomic DNA. Translation: AED94953.1.
CP002688 Genomic DNA. Translation: AED94954.1.
AY136464 mRNA. Translation: AAM97129.1.
BT002143 mRNA. Translation: AAN72154.1.
RefSeqiNP_001032000.1. NM_001036923.1. [O82733-3]
NP_568625.1. NM_123704.3. [O82733-2]
NP_851123.1. NM_180792.3. [O82733-1]
UniGeneiAt.1535.

3D structure databases

ProteinModelPortaliO82733.
SMRiO82733.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19606. 4 interactors.
IntActiO82733. 3 interactors.
STRINGi3702.AT5G43380.1.

Proteomic databases

PaxDbiO82733.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G43380.1; AT5G43380.1; AT5G43380. [O82733-1]
GeneIDi834356.
GrameneiAT5G43380.1; AT5G43380.1; AT5G43380.
KEGGiath:AT5G43380.

Organism-specific databases

TAIRiAT5G43380.

Phylogenomic databases

eggNOGiKOG0374. Eukaryota.
COG0639. LUCA.
HOGENOMiHOG000172697.
InParanoidiO82733.
KOiK06269.
OMAiGFEFFAN.
OrthoDBiEOG09360FO9.
PhylomeDBiO82733.

Miscellaneous databases

PROiO82733.

Gene expression databases

GenevisibleiO82733. AT.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPP17_ARATH
AccessioniPrimary (citable) accession number: O82733
Secondary accession number(s): O48641, Q2V319, Q8L761
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 21, 2003
Last modified: November 30, 2016
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.