Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O82733

- PP17_ARATH

UniProt

O82733 - PP17_ARATH

Protein

Serine/threonine-protein phosphatase PP1 isozyme 7

Gene

TOPP7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 3 (21 Nov 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi60 – 601Manganese 1By similarity
    Metal bindingi62 – 621Manganese 1By similarity
    Metal bindingi88 – 881Manganese 1By similarity
    Metal bindingi88 – 881Manganese 2By similarity
    Metal bindingi120 – 1201Manganese 2By similarity
    Active sitei121 – 1211Proton donorBy similarity
    Metal bindingi169 – 1691Manganese 2By similarity
    Metal bindingi244 – 2441Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphoprotein phosphatase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G43380-MONOMER.
    ARA:GQT-2659-MONOMER.
    ARA:GQT-2660-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PP1 isozyme 7 (EC:3.1.3.16)
    Gene namesi
    Name:TOPP7
    Ordered Locus Names:At5g43380
    ORF Names:MWF20.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G43380.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 331331Serine/threonine-protein phosphatase PP1 isozyme 7PRO_0000058803Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiO82733.
    PRIDEiO82733.

    Expressioni

    Tissue specificityi

    Expressed in roots, rosettes and flowers.

    Gene expression databases

    GenevestigatoriO82733.

    Interactioni

    Protein-protein interaction databases

    BioGridi19606. 3 interactions.
    IntActiO82733. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliO82733.
    SMRiO82733. Positions 6-293.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000172697.
    InParanoidiO82733.
    KOiK06269.
    OMAiGFEFFAN.
    PhylomeDBiO82733.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O82733-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDPGTLNSVI NRLLEAREKP GKIVQLSETE IKQLCFVSRD IFLRQPNLLE    50
    LEAPVKICGD IHGQYPDLLR LFEHGGYPPN SNYLFLGDYV DRGKQSLETI 100
    CLLLAYKIKF PENFFLLRGN HESASINRIY GFYDECKRRF SVKIWRIFTD 150
    CFNCLPVAAL IDERIFCMHG GLSPELLSLR QIRDIRRPTD IPDRGLLCDL 200
    LWSDPDKDVR GWGPNDRGVS YTFGSDIVSG FLKRLDLDLI CRAHQVVEDG 250
    FEFFANKQLV TIFSAPNYCG EFDNAGAMMS VSEDLTCSFQ ILKSNDKKSK 300
    FSFGSRGGAK TSFPYPKVKS ILSSQNSKEY N 331
    Length:331
    Mass (Da):37,705
    Last modified:November 21, 2003 - v3
    Checksum:i2D351A1C368B0CA2
    GO
    Isoform 2 (identifier: O82733-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         320-331: SILSSQNSKEYN → VCINHITF

    Show »
    Length:327
    Mass (Da):37,282
    Checksum:iCBEEFA363F8C635A
    GO
    Isoform 3 (identifier: O82733-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         320-331: SILSSQNSKEYN → DCNW

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:323
    Mass (Da):36,872
    Checksum:iF8B87342B8CC118B
    GO

    Sequence cautioni

    The sequence AAC39459.1 differs from that shown. Reason: Frameshift at position 314.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti7 – 71N → I in AAC39459. (PubMed:9617814)Curated
    Sequence conflicti76 – 761G → E in AAC39459. (PubMed:9617814)Curated
    Sequence conflicti84 – 841Missing in AAC39459. (PubMed:9617814)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei320 – 33112SILSS…SKEYN → VCINHITF in isoform 2. 1 PublicationVSP_009006Add
    BLAST
    Alternative sequencei320 – 33112SILSS…SKEYN → DCNW in isoform 3. CuratedVSP_028726Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB000094 Genomic DNA. Translation: BAA24283.1.
    U80920 mRNA. Translation: AAC39459.1. Frameshift.
    AB025638 Genomic DNA. Translation: BAA97417.1.
    CP002688 Genomic DNA. Translation: AED94952.1.
    CP002688 Genomic DNA. Translation: AED94953.1.
    CP002688 Genomic DNA. Translation: AED94954.1.
    AY136464 mRNA. Translation: AAM97129.1.
    BT002143 mRNA. Translation: AAN72154.1.
    RefSeqiNP_001032000.1. NM_001036923.1. [O82733-3]
    NP_568625.1. NM_123704.2. [O82733-2]
    NP_851123.1. NM_180792.2. [O82733-1]
    UniGeneiAt.1535.

    Genome annotation databases

    EnsemblPlantsiAT5G43380.1; AT5G43380.1; AT5G43380. [O82733-1]
    GeneIDi834356.
    KEGGiath:AT5G43380.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB000094 Genomic DNA. Translation: BAA24283.1 .
    U80920 mRNA. Translation: AAC39459.1 . Frameshift.
    AB025638 Genomic DNA. Translation: BAA97417.1 .
    CP002688 Genomic DNA. Translation: AED94952.1 .
    CP002688 Genomic DNA. Translation: AED94953.1 .
    CP002688 Genomic DNA. Translation: AED94954.1 .
    AY136464 mRNA. Translation: AAM97129.1 .
    BT002143 mRNA. Translation: AAN72154.1 .
    RefSeqi NP_001032000.1. NM_001036923.1. [O82733-3 ]
    NP_568625.1. NM_123704.2. [O82733-2 ]
    NP_851123.1. NM_180792.2. [O82733-1 ]
    UniGenei At.1535.

    3D structure databases

    ProteinModelPortali O82733.
    SMRi O82733. Positions 6-293.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 19606. 3 interactions.
    IntActi O82733. 3 interactions.

    Proteomic databases

    PaxDbi O82733.
    PRIDEi O82733.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G43380.1 ; AT5G43380.1 ; AT5G43380 . [O82733-1 ]
    GeneIDi 834356.
    KEGGi ath:AT5G43380.

    Organism-specific databases

    TAIRi AT5G43380.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000172697.
    InParanoidi O82733.
    KOi K06269.
    OMAi GFEFFAN.
    PhylomeDBi O82733.

    Enzyme and pathway databases

    BioCyci ARA:AT5G43380-MONOMER.
    ARA:GQT-2659-MONOMER.
    ARA:GQT-2660-MONOMER.

    Gene expression databases

    Genevestigatori O82733.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a gene that encode a protein phosphatase 1 catalytic subunit in Arabidopsis thaliana."
      Mitsukawa N., Okumura S., Shibata D.
      Soil Sci. Plant Nutr. 43:971-974(1997)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
      Strain: cv. Columbia.
    2. "Molecular cloning and chromosomal mapping of type one serine/threonine protein phosphatases in Arabidopsis thaliana."
      Lin Q., Li J., Smith R.D., Walker J.C.
      Plant Mol. Biol. 37:471-481(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
      DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    6. "Arabidopsis PPP family of serine/threonine phosphatases."
      Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
      Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPP17_ARATH
    AccessioniPrimary (citable) accession number: O82733
    Secondary accession number(s): O48641, Q2V319, Q8L761
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 21, 2003
    Last modified: October 1, 2014
    This is version 118 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3