O82730 (MGDG2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Monogalactosyldiacylglycerol synthase 2, chloroplastic
Short name=AtMGD2
EC=2.4.1.46

Alternative name(s):
MGDG synthase type B

Gene names

Name:	MGD2
Synonyms:	MGDB
Ordered Locus Names:	At5g20410
ORF Names:	F5O24.300, F7C8

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the synthesis of the major structural component of photosynthetic membranes and in the chloroplast envelope biogenesis. Can use both prokaryotic (18:1/16:0) or eukaryotic (18:2/18:2) 1,2-diacylglycerol species, but operates with some preference for the eukaryotic one. Ref.1
Catalytic activity	UDP-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.
Subcellular location	Plastid › chloroplast outer membrane Probable Ref.1.
Tissue specificity	Expressed mainly in floral buds. Detected in roots, leaves, stems, siliques and pollen tubes. Ref.1 Ref.5
Developmental stage	Low and continuous expression throughout whole developmental stages. Ref.1
Induction	Induced by phosphate deprivation. Ref.1 Ref.5 Ref.6
Miscellaneous	Auxin activates expression during Pi starvation, whereas cytokinin represses it.
Sequence similarities	Belongs to the glycosyltransferase 28 family.
Biophysicochemical properties	pH dependence: Optimum pH is 8.5.
Sequence caution	The sequence AAK50066.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Chloroplast Membrane Plastid Plastid outer membrane
Domain	Transit peptide
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to phosphate starvation Inferred from genetic interaction. Source: TAIR fatty acid metabolic process Inferred from genetic interaction. Source: TAIR galactolipid metabolic process Inferred from genetic interaction. Source: TAIR glycolipid biosynthetic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast outer membrane Traceable author statement. Source: TAIR
Molecular function	1,2-diacylglycerol 3-beta-galactosyltransferase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 468		Monogalactosyldiacylglycerol synthase 2, chloroplastic		PRO_0000349422

Sequences

	Sequence		Length	Mass (Da)
	O82730 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 58F56C3C1C383C24	FASTA	468	52,727
	10 20 30 40 50 60 MATTVMALAE KVLERVYGTS KSAVSVTSGD GEKTHRHTHH HIHRIKSYDD IDEDESSLEL 70 80 90 100 110 120 IQIGAERTKN VLILMSDTGG GHRASAEAIR DAFKIEFGDK YRVIVKDVWK EYTGWPLNDM 130 140 150 160 170 180 ERSYKFMVKH VQLWKVAFHS TSPKWIHSCY LAAIAAYYAK EVEAGLMEYK PEIIISVHPL 190 200 210 220 230 240 MQHIPLWVLK WQELQKRVLF VTVITDLNTC HPTWFHPGVN RCYCPSQEVA KRALFDGLDE 250 260 270 280 290 300 SQVRVFGLPV RPSFARAVLV KDDLRKELEM DQDLRAVLLM GGGEGMGPVK ETAKALEEFL 310 320 330 340 350 360 YDKENRKPIG QMVVICGRNK KLASALEAID WKIPVKVRGF ETQMEKWMGA CDCIITKAGP 370 380 390 400 410 420 GTIAESLIRS LPIILNDYIP GQEKGNVPYV VENGAGVFTR SPKETARIVG EWFSTKTDEL 430 440 450 460 EQTSDNARKL AQPEAVFDIV KDIDELSEQR GPLASVSYNL TSSFASLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana." Awai K., Marechal E., Block M.A., Brun D., Masuda T., Shimada H., Takamiya K., Ohta H., Joyard J. Proc. Natl. Acad. Sci. U.S.A. 98:10960-10965(2001) [PubMed: 11553816] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 10-468. Strain: cv. Columbia.
[5]	"Arabidopsis type B monogalactosyldiacylglycerol synthase genes are expressed during pollen tube growth and induced by phosphate starvation." Kobayashi K., Awai K., Takamiya K., Ohta H. Plant Physiol. 134:640-648(2004) [PubMed: 14730084] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[6]	"Membrane lipid alteration during phosphate starvation is regulated by phosphate signaling and auxin/cytokinin cross-talk." Kobayashi K., Masuda T., Takamiya K., Ohta H. Plant J. 47:238-248(2006) [PubMed: 16762032] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ000331 mRNA. Translation: CAA04005.1. AF296825 Genomic DNA. No translation available. AF296833 Genomic DNA. No translation available. CP002688 Genomic DNA. Translation: AED92840.1. AF372926 mRNA. Translation: AAK50066.1. Different initiation. AY078038 mRNA. Translation: AAL77739.1.
IPI	IPI00546173.
PIR	T52269.
RefSeq	NP_568394.2. NM_122048.3.
UniGene	At.5393.
3D structure databases
ProteinModelPortal	O82730.
ModBase	Search...
Protein-protein interaction databases
STRING	O82730.
Protein family/group databases
CAZy	GT28. Glycosyltransferase Family 28.
Proteomic databases
PRIDE	O82730.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G20410.1; AT5G20410.1; AT5G20410.
GeneID	832163.
GenomeReviews	Gene locus AT5G20410 in contig BA000015_GR.
KEGG	ath:AT5G20410.
Organism-specific databases
TAIR	At5g20410.
Phylogenomic databases
eggNOG	COG0707.
GeneTree	EPGT00050000017026.
HOGENOM	HBG601852.
InParanoid	O82730.
OMA	GVNRCYC.
PhylomeDB	O82730.
ProtClustDB	PLN02605.
Enzyme and pathway databases
BRENDA	2.4.1.46. 399.
Gene expression databases
Genevestigator	O82730.
Family and domain databases
InterPro	IPR009695. Diacylglyc_glucosyltr_N. IPR007235. Glyco_trans_28_C. [Graphical view]
KO	K03715.
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF06925. MGDG_synth. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGDG2_ARATH

Accession

Primary (citable) accession number: O82730
Secondary accession number(s): Q94JU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	November 1, 1998
Last modified:	December 14, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program