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O82730

- MGDG2_ARATH

UniProt

O82730 - MGDG2_ARATH

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Protein
Monogalactosyldiacylglycerol synthase 2, chloroplastic
Gene
MGD2, MGDB, At5g20410, F5O24.300, F7C8
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the major structural component of photosynthetic membranes and in the chloroplast envelope biogenesis. Can use both prokaryotic (18:1/16:0) or eukaryotic (18:2/18:2) 1,2-diacylglycerol species, but operates with some preference for the eukaryotic one.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.

pH dependencei

Optimum pH is 8.5.

GO - Molecular functioni

  1. 1,2-diacylglycerol 3-beta-galactosyltransferase activity Source: UniProtKB-EC
  2. UDP-galactosyltransferase activity Source: TAIR
  3. carbohydrate binding Source: InterPro

GO - Biological processi

  1. cellular response to phosphate starvation Source: TAIR
  2. fatty acid metabolic process Source: TAIR
  3. galactolipid metabolic process Source: TAIR
  4. glycolipid biosynthetic process Source: InterPro
  5. lipid glycosylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:AT5G20410-MONOMER.
BRENDAi2.4.1.46. 399.

Protein family/group databases

CAZyiGT28. Glycosyltransferase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Monogalactosyldiacylglycerol synthase 2, chloroplastic (EC:2.4.1.46)
Short name:
AtMGD2
Alternative name(s):
MGDG synthase type B
Gene namesi
Name:MGD2
Synonyms:MGDB
Ordered Locus Names:At5g20410
ORF Names:F5O24.300, F7C8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G20410.

Subcellular locationi

Plastidchloroplast outer membrane Inferred 1 Publication

GO - Cellular componenti

  1. chloroplast outer membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 468Monogalactosyldiacylglycerol synthase 2, chloroplasticPRO_0000349422
Transit peptidei1 – ?Chloroplast Reviewed prediction

Proteomic databases

PRIDEiO82730.

Expressioni

Tissue specificityi

Expressed mainly in floral buds. Detected in roots, leaves, stems, siliques and pollen tubes.2 Publications

Developmental stagei

Low and continuous expression throughout whole developmental stages.1 Publication

Inductioni

Induced by phosphate deprivation.3 Publications

Gene expression databases

GenevestigatoriO82730.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G20410.1-P.

Structurei

3D structure databases

ProteinModelPortaliO82730.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0707.
HOGENOMiHOG000239591.
InParanoidiO82730.
KOiK03715.
OMAiKRMSENA.
PhylomeDBiO82730.

Family and domain databases

InterProiIPR009695. Diacylglyc_glucosyltr_N.
IPR007235. Glyco_trans_28_C.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O82730-1 [UniParc]FASTAAdd to Basket

« Hide

MATTVMALAE KVLERVYGTS KSAVSVTSGD GEKTHRHTHH HIHRIKSYDD    50
IDEDESSLEL IQIGAERTKN VLILMSDTGG GHRASAEAIR DAFKIEFGDK 100
YRVIVKDVWK EYTGWPLNDM ERSYKFMVKH VQLWKVAFHS TSPKWIHSCY 150
LAAIAAYYAK EVEAGLMEYK PEIIISVHPL MQHIPLWVLK WQELQKRVLF 200
VTVITDLNTC HPTWFHPGVN RCYCPSQEVA KRALFDGLDE SQVRVFGLPV 250
RPSFARAVLV KDDLRKELEM DQDLRAVLLM GGGEGMGPVK ETAKALEEFL 300
YDKENRKPIG QMVVICGRNK KLASALEAID WKIPVKVRGF ETQMEKWMGA 350
CDCIITKAGP GTIAESLIRS LPIILNDYIP GQEKGNVPYV VENGAGVFTR 400
SPKETARIVG EWFSTKTDEL EQTSDNARKL AQPEAVFDIV KDIDELSEQR 450
GPLASVSYNL TSSFASLV 468
Length:468
Mass (Da):52,727
Last modified:November 1, 1998 - v1
Checksum:i58F56C3C1C383C24
GO

Sequence cautioni

The sequence AAK50066.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ000331 mRNA. Translation: CAA04005.1.
AF296825 Genomic DNA. No translation available.
AF296833 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92840.1.
AF372926 mRNA. Translation: AAK50066.1. Different initiation.
AY078038 mRNA. Translation: AAL77739.1.
PIRiT52269.
RefSeqiNP_568394.2. NM_122048.3.
UniGeneiAt.5393.

Genome annotation databases

EnsemblPlantsiAT5G20410.1; AT5G20410.1; AT5G20410.
GeneIDi832163.
KEGGiath:AT5G20410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ000331 mRNA. Translation: CAA04005.1 .
AF296825 Genomic DNA. No translation available.
AF296833 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92840.1 .
AF372926 mRNA. Translation: AAK50066.1 . Different initiation.
AY078038 mRNA. Translation: AAL77739.1 .
PIRi T52269.
RefSeqi NP_568394.2. NM_122048.3.
UniGenei At.5393.

3D structure databases

ProteinModelPortali O82730.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G20410.1-P.

Protein family/group databases

CAZyi GT28. Glycosyltransferase Family 28.

Proteomic databases

PRIDEi O82730.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G20410.1 ; AT5G20410.1 ; AT5G20410 .
GeneIDi 832163.
KEGGi ath:AT5G20410.

Organism-specific databases

TAIRi AT5G20410.

Phylogenomic databases

eggNOGi COG0707.
HOGENOMi HOG000239591.
InParanoidi O82730.
KOi K03715.
OMAi KRMSENA.
PhylomeDBi O82730.

Enzyme and pathway databases

BioCyci MetaCyc:AT5G20410-MONOMER.
BRENDAi 2.4.1.46. 399.

Gene expression databases

Genevestigatori O82730.

Family and domain databases

InterProi IPR009695. Diacylglyc_glucosyltr_N.
IPR007235. Glyco_trans_28_C.
[Graphical view ]
Pfami PF04101. Glyco_tran_28_C. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana."
    Awai K., Marechal E., Block M.A., Brun D., Masuda T., Shimada H., Takamiya K., Ohta H., Joyard J.
    Proc. Natl. Acad. Sci. U.S.A. 98:10960-10965(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 10-468.
    Strain: cv. Columbia.
  5. "Arabidopsis type B monogalactosyldiacylglycerol synthase genes are expressed during pollen tube growth and induced by phosphate starvation."
    Kobayashi K., Awai K., Takamiya K., Ohta H.
    Plant Physiol. 134:640-648(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  6. "Membrane lipid alteration during phosphate starvation is regulated by phosphate signaling and auxin/cytokinin cross-talk."
    Kobayashi K., Masuda T., Takamiya K., Ohta H.
    Plant J. 47:238-248(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiMGDG2_ARATH
AccessioniPrimary (citable) accession number: O82730
Secondary accession number(s): Q94JU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: November 1, 1998
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Auxin activates expression during Pi starvation, whereas cytokinin represses it.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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