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O82730 (MGDG2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Monogalactosyldiacylglycerol synthase 2, chloroplastic

Short name=AtMGD2
EC=2.4.1.46
Alternative name(s):
MGDG synthase type B
Gene names
Name:MGD2
Synonyms:MGDB
Ordered Locus Names:At5g20410
ORF Names:F5O24.300, F7C8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the synthesis of the major structural component of photosynthetic membranes and in the chloroplast envelope biogenesis. Can use both prokaryotic (18:1/16:0) or eukaryotic (18:2/18:2) 1,2-diacylglycerol species, but operates with some preference for the eukaryotic one. Ref.1

Catalytic activity

UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.

Subcellular location

Plastidchloroplast outer membrane Probable Ref.1.

Tissue specificity

Expressed mainly in floral buds. Detected in roots, leaves, stems, siliques and pollen tubes. Ref.1 Ref.5

Developmental stage

Low and continuous expression throughout whole developmental stages. Ref.1

Induction

Induced by phosphate deprivation. Ref.1 Ref.5 Ref.6

Miscellaneous

Auxin activates expression during Pi starvation, whereas cytokinin represses it.

Sequence similarities

Belongs to the glycosyltransferase 28 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 8.5.

Sequence caution

The sequence AAK50066.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 468Monogalactosyldiacylglycerol synthase 2, chloroplasticPRO_0000349422

Sequences

Sequence LengthMass (Da)Tools
O82730 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 58F56C3C1C383C24

FASTA46852,727
        10         20         30         40         50         60 
MATTVMALAE KVLERVYGTS KSAVSVTSGD GEKTHRHTHH HIHRIKSYDD IDEDESSLEL 

        70         80         90        100        110        120 
IQIGAERTKN VLILMSDTGG GHRASAEAIR DAFKIEFGDK YRVIVKDVWK EYTGWPLNDM 

       130        140        150        160        170        180 
ERSYKFMVKH VQLWKVAFHS TSPKWIHSCY LAAIAAYYAK EVEAGLMEYK PEIIISVHPL 

       190        200        210        220        230        240 
MQHIPLWVLK WQELQKRVLF VTVITDLNTC HPTWFHPGVN RCYCPSQEVA KRALFDGLDE 

       250        260        270        280        290        300 
SQVRVFGLPV RPSFARAVLV KDDLRKELEM DQDLRAVLLM GGGEGMGPVK ETAKALEEFL 

       310        320        330        340        350        360 
YDKENRKPIG QMVVICGRNK KLASALEAID WKIPVKVRGF ETQMEKWMGA CDCIITKAGP 

       370        380        390        400        410        420 
GTIAESLIRS LPIILNDYIP GQEKGNVPYV VENGAGVFTR SPKETARIVG EWFSTKTDEL 

       430        440        450        460 
EQTSDNARKL AQPEAVFDIV KDIDELSEQR GPLASVSYNL TSSFASLV 

« Hide

References

« Hide 'large scale' references
[1]"Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana."
Awai K., Marechal E., Block M.A., Brun D., Masuda T., Shimada H., Takamiya K., Ohta H., Joyard J.
Proc. Natl. Acad. Sci. U.S.A. 98:10960-10965(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 10-468.
Strain: cv. Columbia.
[5]"Arabidopsis type B monogalactosyldiacylglycerol synthase genes are expressed during pollen tube growth and induced by phosphate starvation."
Kobayashi K., Awai K., Takamiya K., Ohta H.
Plant Physiol. 134:640-648(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
[6]"Membrane lipid alteration during phosphate starvation is regulated by phosphate signaling and auxin/cytokinin cross-talk."
Kobayashi K., Masuda T., Takamiya K., Ohta H.
Plant J. 47:238-248(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ000331 mRNA. Translation: CAA04005.1.
AF296825 Genomic DNA. No translation available.
AF296833 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92840.1.
AF372926 mRNA. Translation: AAK50066.1. Different initiation.
AY078038 mRNA. Translation: AAL77739.1.
PIRT52269.
RefSeqNP_568394.2. NM_122048.3.
UniGeneAt.5393.

3D structure databases

ProteinModelPortalO82730.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G20410.1-P.

Protein family/group databases

CAZyGT28. Glycosyltransferase Family 28.

Proteomic databases

PRIDEO82730.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G20410.1; AT5G20410.1; AT5G20410.
GeneID832163.
KEGGath:AT5G20410.

Organism-specific databases

TAIRAT5G20410.

Phylogenomic databases

eggNOGCOG0707.
HOGENOMHOG000239591.
InParanoidO82730.
KOK03715.
OMASYKFMVK.
PhylomeDBO82730.
ProtClustDBPLN02605.

Enzyme and pathway databases

BioCycMetaCyc:AT5G20410-MONOMER.
BRENDA2.4.1.46. 399.

Gene expression databases

GenevestigatorO82730.

Family and domain databases

InterProIPR009695. Diacylglyc_glucosyltr_N.
IPR007235. Glyco_trans_28_C.
[Graphical view]
PfamPF04101. Glyco_tran_28_C. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMGDG2_ARATH
AccessionPrimary (citable) accession number: O82730
Secondary accession number(s): Q94JU8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: November 1, 1998
Last modified: February 19, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names