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Protein

Monogalactosyldiacylglycerol synthase 2, chloroplastic

Gene

MGD2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the major structural component of photosynthetic membranes and in the chloroplast envelope biogenesis. Can use both prokaryotic (18:1/16:0) or eukaryotic (18:2/18:2) 1,2-diacylglycerol species, but operates with some preference for the eukaryotic one.1 Publication

Miscellaneous

Auxin activates expression during Pi starvation, whereas cytokinin represses it.

Catalytic activityi

UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.

pH dependencei

Optimum pH is 8.5.

GO - Molecular functioni

  • 1,2-diacylglycerol 3-beta-galactosyltransferase activity Source: UniProtKB-EC
  • UDP-galactosyltransferase activity Source: TAIR

GO - Biological processi

  • cellular response to phosphate starvation Source: TAIR
  • fatty acid metabolic process Source: TAIR
  • galactolipid metabolic process Source: TAIR
  • glycolipid biosynthetic process Source: InterPro

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:AT5G20410-MONOMER
BRENDAi2.4.1.46 399

Protein family/group databases

CAZyiGT28 Glycosyltransferase Family 28

Chemistry databases

SwissLipidsiSLP:000001445

Names & Taxonomyi

Protein namesi
Recommended name:
Monogalactosyldiacylglycerol synthase 2, chloroplastic (EC:2.4.1.46)
Short name:
AtMGD2
Alternative name(s):
MGDG synthase type B
Gene namesi
Name:MGD2
Synonyms:MGDB
Ordered Locus Names:At5g20410
ORF Names:F5O24.300, F7C8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G20410
TAIRilocus:2149274 AT5G20410

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000349422? – 468Monogalactosyldiacylglycerol synthase 2, chloroplastic
Transit peptidei1 – ?ChloroplastSequence analysis

Proteomic databases

PaxDbiO82730

PTM databases

iPTMnetiO82730

Expressioni

Tissue specificityi

Expressed mainly in floral buds. Detected in roots, leaves, stems, siliques and pollen tubes.2 Publications

Developmental stagei

Low and continuous expression throughout whole developmental stages.1 Publication

Inductioni

Induced by phosphate deprivation.3 Publications

Gene expression databases

ExpressionAtlasiO82730 baseline and differential
GenevisibleiO82730 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G20410.1

Structurei

3D structure databases

ProteinModelPortaliO82730
SMRiO82730
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 28 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IKN8 Eukaryota
COG0707 LUCA
HOGENOMiHOG000239591
InParanoidiO82730
KOiK03715
OMAiSPKWIHS
OrthoDBiEOG09360AEE
PhylomeDBiO82730

Family and domain databases

InterProiView protein in InterPro
IPR009695 Diacylglyc_glucosyltr_N
IPR007235 Glyco_trans_28_C
PfamiView protein in Pfam
PF04101 Glyco_tran_28_C, 1 hit
PF06925 MGDG_synth, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O82730-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTVMALAE KVLERVYGTS KSAVSVTSGD GEKTHRHTHH HIHRIKSYDD
60 70 80 90 100
IDEDESSLEL IQIGAERTKN VLILMSDTGG GHRASAEAIR DAFKIEFGDK
110 120 130 140 150
YRVIVKDVWK EYTGWPLNDM ERSYKFMVKH VQLWKVAFHS TSPKWIHSCY
160 170 180 190 200
LAAIAAYYAK EVEAGLMEYK PEIIISVHPL MQHIPLWVLK WQELQKRVLF
210 220 230 240 250
VTVITDLNTC HPTWFHPGVN RCYCPSQEVA KRALFDGLDE SQVRVFGLPV
260 270 280 290 300
RPSFARAVLV KDDLRKELEM DQDLRAVLLM GGGEGMGPVK ETAKALEEFL
310 320 330 340 350
YDKENRKPIG QMVVICGRNK KLASALEAID WKIPVKVRGF ETQMEKWMGA
360 370 380 390 400
CDCIITKAGP GTIAESLIRS LPIILNDYIP GQEKGNVPYV VENGAGVFTR
410 420 430 440 450
SPKETARIVG EWFSTKTDEL EQTSDNARKL AQPEAVFDIV KDIDELSEQR
460
GPLASVSYNL TSSFASLV
Length:468
Mass (Da):52,727
Last modified:November 1, 1998 - v1
Checksum:i58F56C3C1C383C24
GO

Sequence cautioni

The sequence AAK50066 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000331 mRNA Translation: CAA04005.1
KJ138675 mRNA Translation: AHL38615.1
AF296825 Genomic DNA No translation available.
AF296833 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED92840.1
AF372926 mRNA Translation: AAK50066.1 Different initiation.
AY078038 mRNA Translation: AAL77739.1
PIRiT52269
RefSeqiNP_568394.2, NM_122048.4
UniGeneiAt.5393

Genome annotation databases

EnsemblPlantsiAT5G20410.1; AT5G20410.1; AT5G20410
GeneIDi832163
GrameneiAT5G20410.1; AT5G20410.1; AT5G20410
KEGGiath:AT5G20410

Similar proteinsi

Entry informationi

Entry nameiMGDG2_ARATH
AccessioniPrimary (citable) accession number: O82730
Secondary accession number(s): Q94JU8, W8Q2S4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health