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O82730

- MGDG2_ARATH

UniProt

O82730 - MGDG2_ARATH

Protein

Monogalactosyldiacylglycerol synthase 2, chloroplastic

Gene

MGD2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Involved in the synthesis of the major structural component of photosynthetic membranes and in the chloroplast envelope biogenesis. Can use both prokaryotic (18:1/16:0) or eukaryotic (18:2/18:2) 1,2-diacylglycerol species, but operates with some preference for the eukaryotic one.1 Publication

    Catalytic activityi

    UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.

    pH dependencei

    Optimum pH is 8.5.

    GO - Molecular functioni

    1. 1,2-diacylglycerol 3-beta-galactosyltransferase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. UDP-galactosyltransferase activity Source: TAIR

    GO - Biological processi

    1. cellular response to phosphate starvation Source: TAIR
    2. fatty acid metabolic process Source: TAIR
    3. galactolipid metabolic process Source: TAIR
    4. glycolipid biosynthetic process Source: InterPro
    5. lipid glycosylation Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:AT5G20410-MONOMER.
    BRENDAi2.4.1.46. 399.

    Protein family/group databases

    CAZyiGT28. Glycosyltransferase Family 28.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Monogalactosyldiacylglycerol synthase 2, chloroplastic (EC:2.4.1.46)
    Short name:
    AtMGD2
    Alternative name(s):
    MGDG synthase type B
    Gene namesi
    Name:MGD2
    Synonyms:MGDB
    Ordered Locus Names:At5g20410
    ORF Names:F5O24.300, F7C8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G20410.

    Subcellular locationi

    Plastidchloroplast outer membrane 1 Publication

    GO - Cellular componenti

    1. chloroplast outer membrane Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Plastid outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 468Monogalactosyldiacylglycerol synthase 2, chloroplasticPRO_0000349422
    Transit peptidei1 – ?ChloroplastSequence Analysis

    Proteomic databases

    PRIDEiO82730.

    Expressioni

    Tissue specificityi

    Expressed mainly in floral buds. Detected in roots, leaves, stems, siliques and pollen tubes.2 Publications

    Developmental stagei

    Low and continuous expression throughout whole developmental stages.1 Publication

    Inductioni

    Induced by phosphate deprivation.3 Publications

    Gene expression databases

    GenevestigatoriO82730.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G20410.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO82730.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 28 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0707.
    HOGENOMiHOG000239591.
    InParanoidiO82730.
    KOiK03715.
    OMAiKRMSENA.
    PhylomeDBiO82730.

    Family and domain databases

    InterProiIPR009695. Diacylglyc_glucosyltr_N.
    IPR007235. Glyco_trans_28_C.
    [Graphical view]
    PfamiPF04101. Glyco_tran_28_C. 1 hit.
    PF06925. MGDG_synth. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O82730-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATTVMALAE KVLERVYGTS KSAVSVTSGD GEKTHRHTHH HIHRIKSYDD    50
    IDEDESSLEL IQIGAERTKN VLILMSDTGG GHRASAEAIR DAFKIEFGDK 100
    YRVIVKDVWK EYTGWPLNDM ERSYKFMVKH VQLWKVAFHS TSPKWIHSCY 150
    LAAIAAYYAK EVEAGLMEYK PEIIISVHPL MQHIPLWVLK WQELQKRVLF 200
    VTVITDLNTC HPTWFHPGVN RCYCPSQEVA KRALFDGLDE SQVRVFGLPV 250
    RPSFARAVLV KDDLRKELEM DQDLRAVLLM GGGEGMGPVK ETAKALEEFL 300
    YDKENRKPIG QMVVICGRNK KLASALEAID WKIPVKVRGF ETQMEKWMGA 350
    CDCIITKAGP GTIAESLIRS LPIILNDYIP GQEKGNVPYV VENGAGVFTR 400
    SPKETARIVG EWFSTKTDEL EQTSDNARKL AQPEAVFDIV KDIDELSEQR 450
    GPLASVSYNL TSSFASLV 468
    Length:468
    Mass (Da):52,727
    Last modified:November 1, 1998 - v1
    Checksum:i58F56C3C1C383C24
    GO

    Sequence cautioni

    The sequence AAK50066.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ000331 mRNA. Translation: CAA04005.1.
    AF296825 Genomic DNA. No translation available.
    AF296833 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED92840.1.
    AF372926 mRNA. Translation: AAK50066.1. Different initiation.
    AY078038 mRNA. Translation: AAL77739.1.
    PIRiT52269.
    RefSeqiNP_568394.2. NM_122048.3.
    UniGeneiAt.5393.

    Genome annotation databases

    EnsemblPlantsiAT5G20410.1; AT5G20410.1; AT5G20410.
    GeneIDi832163.
    KEGGiath:AT5G20410.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ000331 mRNA. Translation: CAA04005.1 .
    AF296825 Genomic DNA. No translation available.
    AF296833 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED92840.1 .
    AF372926 mRNA. Translation: AAK50066.1 . Different initiation.
    AY078038 mRNA. Translation: AAL77739.1 .
    PIRi T52269.
    RefSeqi NP_568394.2. NM_122048.3.
    UniGenei At.5393.

    3D structure databases

    ProteinModelPortali O82730.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G20410.1-P.

    Protein family/group databases

    CAZyi GT28. Glycosyltransferase Family 28.

    Proteomic databases

    PRIDEi O82730.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G20410.1 ; AT5G20410.1 ; AT5G20410 .
    GeneIDi 832163.
    KEGGi ath:AT5G20410.

    Organism-specific databases

    TAIRi AT5G20410.

    Phylogenomic databases

    eggNOGi COG0707.
    HOGENOMi HOG000239591.
    InParanoidi O82730.
    KOi K03715.
    OMAi KRMSENA.
    PhylomeDBi O82730.

    Enzyme and pathway databases

    BioCyci MetaCyc:AT5G20410-MONOMER.
    BRENDAi 2.4.1.46. 399.

    Gene expression databases

    Genevestigatori O82730.

    Family and domain databases

    InterProi IPR009695. Diacylglyc_glucosyltr_N.
    IPR007235. Glyco_trans_28_C.
    [Graphical view ]
    Pfami PF04101. Glyco_tran_28_C. 1 hit.
    PF06925. MGDG_synth. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana."
      Awai K., Marechal E., Block M.A., Brun D., Masuda T., Shimada H., Takamiya K., Ohta H., Joyard J.
      Proc. Natl. Acad. Sci. U.S.A. 98:10960-10965(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 10-468.
      Strain: cv. Columbia.
    5. "Arabidopsis type B monogalactosyldiacylglycerol synthase genes are expressed during pollen tube growth and induced by phosphate starvation."
      Kobayashi K., Awai K., Takamiya K., Ohta H.
      Plant Physiol. 134:640-648(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.
    6. "Membrane lipid alteration during phosphate starvation is regulated by phosphate signaling and auxin/cytokinin cross-talk."
      Kobayashi K., Masuda T., Takamiya K., Ohta H.
      Plant J. 47:238-248(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiMGDG2_ARATH
    AccessioniPrimary (citable) accession number: O82730
    Secondary accession number(s): Q94JU8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Auxin activates expression during Pi starvation, whereas cytokinin represses it.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3